Abstract
L.) is not fully understood. Accumulation of ureide in soybean plant tissues may regulate the nitrogen fixation capacity through a feedback pathway. In this study, unilaterally nodulated dual-root soybeans prepared by grafting were grown in sand culture. They were subjected to the removal of the nodulated side roots, and were given either nitrate supply or no supply to the non-nodulated side roots for 3 days (experiment I). Additionally, they received nitrate supply to the non-nodulated side roots for 1-14 days (experiment II). The results showed that nitrate supply increased the levels of asparagine and ureide in soybean shoots (Experiment I). In Experiment II, nodule dry weight, nodule number, nodule nitrogenase activity, and nodule urate oxidase activity decreased significantly after 3, 7, and 14 days of nitrate supply. Ureide content in the shoots and nodules increased after 1, 3, and 7 days of nitrate supply, but decreased after 14 days of nitrate supply. There was a significant positive correlation between urate oxidase activity and nitrogenase activity. Hence, we deduced that nitrate supply increased the asparagine content in soybean shoots, likely inhibiting ureide degradation, which induced the accumulation of ureide in soybean shoots and nodules, and, in turn, feedback inhibited the nodule nitrogen fixation. In addition, urate oxidase activity can be used to assess the nitrogen fixation capacity of nodules.
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Claims 8
Claim · model-written summary
Nitrate supply increased asparagine and ureide in soybean shoots.
- Taxon
- soybean
Glycine maxe0 - Treatment
- nitrate supplye0
- Outcome
- levels of asparagine and ureide in soybean shootse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that nitrate supply increased the levels of asparagine and ureide in soybean shoots (Experiment I).”
Claim · model-written summary
Nitrate supply significantly decreased nodule dry weight and number after 3, 7, and 14 days.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- nitrate supplye0
- Outcome
- nodule dry weight, nodule numbere0
- Direction
- decreasede0
- Qualifier
- after 3, 7, and 14 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Experiment II, nodule dry weight, nodule number, nodule nitrogenase activity, and nodule urate oxidase activity decreased significantly after 3, 7, and 14 days of nitrate supply.”
t0“Soybean Plants”
Claim · model-written summary
Nitrate supply significantly decreased nodule nitrogenase and urate oxidase activities after 3, 7, and 14 days.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- nitrate supplye0
- Outcome
- nodule nitrogenase activity, and nodule urate oxidase activitye0
- Direction
- decreasede0
- Qualifier
- after 3, 7, and 14 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Experiment II, nodule dry weight, nodule number, nodule nitrogenase activity, and nodule urate oxidase activity decreased significantly after 3, 7, and 14 days of nitrate supply.”
t0“Soybean Plants”
Claim · model-written summary
Shoot and nodule ureide increased after 1, 3, and 7 days of nitrate supply.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- nitrate supplye0
- Outcome
- Ureide content in the shoots and nodulese0
- Direction
- increasede0
- Qualifier
- after 1, 3, and 7 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ureide content in the shoots and nodules increased after 1, 3, and 7 days of nitrate supply, but decreased after 14 days of nitrate supply.”
t0“Soybean Plants”
Claim · model-written summary
Shoot and nodule ureide decreased after 14 days of nitrate supply.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- nitrate supplye0
- Outcome
- Ureide content in the shoots and nodulese0
- Direction
- decreasede0
- Qualifier
- after 14 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ureide content in the shoots and nodules increased after 1, 3, and 7 days of nitrate supply, but decreased after 14 days of nitrate supply.”
t0“Soybean Plants”
Claim · model-written summary
Urate oxidase and nitrogenase activities were positively correlated.
- Taxon
- SoybeanGlycine maxt0
- Gene
- urate oxidasee0
- Outcome
- nitrogenase activitye0
- Direction
- positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was a significant positive correlation between urate oxidase activity and nitrogenase activity.”
t0“Soybean Plants”
Claim · model-written summary
The authors infer that nitrate-induced asparagine inhibits ureide degradation, leading to ureide accumulation and feedback inhibition of fixation.
- Taxon
- soybeanGlycine maxe0
- Treatment
- nitrate supplye0
- Compound
- asparaginee0
- Outcome
- feedback inhibited the nodule nitrogen fixatione0
- Qualifier
- likely inhibiting ureide degradatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hence, we deduced that nitrate supply increased the asparagine content in soybean shoots, likely inhibiting ureide degradation, which induced the accumulation of ureide in soybean shoots and nodules, and, in turn, feedback inhibited the nodule nitrogen fixation.”
Claim · model-written summary
Urate oxidase activity can assess nodule nitrogen fixation capacity.
- Taxon
- SoybeanGlycine maxt0
- Gene
- urate oxidasee0
- Outcome
- assess the nitrogen fixation capacity of nodulese0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition, urate oxidase activity can be used to assess the nitrogen fixation capacity of nodules.”
t0“Soybean Plants”
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