Paper · 2024 · Agrica

Hormonal Manipulation for Rapid Micropropagation of a New Sugarcane Variety CoS 17231

Yukti Gupta; Archana; S. Malhotra; et al.

3
claims
6
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

Effect of growth regulators on the responses of shoot tip explants of new sugarcane variety CoS 17231 was studied during in vitro micropropagation. Shoot cultures were raised from approximately 1.0 cm long shoot tip explants comprising apical meristem and 1-2 leaf primordia on agar (8.0 g/l) solidified Murashige and Skoog’s medium. The established shoot cultures were multiplied on MS medium containing various combinations of BAP, kinetin, NAA and GA3. The results showed that MS medium containing BAP, Kinetin and NAA (0.5 mg/l each) was most suitable for producing optimum number of shoots in this new variety. Maximum 92% shoot cultures developed vigorous root system on ½ strength MS liquid medium containing 50 g/l sucrose and 5.0 mg/l NAA. The rooted shoots were washed, hardened for 45 days in the green house and transplanted to the field. Over 96 % plant survival was recorded 15 days after transplantation.

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Claims 3

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

BAP, kinetin and NAA at 0.5 mg/l each produced the optimum number of shoots.

Taxon
Sugarcanet0
Material
CoS 17231t0
Treatment
MS medium containing BAP, Kinetin and NAA (0.5 mg/l each)e0
Outcome
number of shootse0
Direction
optimume0

Not reportedComparator

Verbatim

e0

“MS medium containing BAP, Kinetin and NAA (0.5 mg/l each) was most suitable for producing optimum number of shoots”

✓ located verbatim in the source · abstract, characters 480–594 · show in text ↑
t0

“Sugarcane Variety CoS 17231”

✓ located verbatim in the source · title, characters 58–85 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Rooting medium yielded vigorous roots in 92% of shoot cultures.

Taxon
Sugarcanet0
Treatment
½ strength MS liquid medium containing 50 g/l sucrose and 5.0 mg/l NAAe0
Outcome
shoot cultures developed vigorous root systeme0
Direction
Maximume0
Value
92%e0

Not reportedComparator

Verbatim

e0

“Maximum 92% shoot cultures developed vigorous root system on ½ strength MS liquid medium containing 50 g/l sucrose and 5.0 mg/l NAA”

✓ located verbatim in the source · abstract, characters 616–747 · show in text ↑
t0

“Sugarcane Variety CoS 17231”

✓ located verbatim in the source · title, characters 58–85 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

More than 96% of transplanted plants survived after 15 days.

Taxon
Sugarcanet0
Outcome
plant survivale0
Value
96 %e0
Qualifier
15 days after transplantatione0

Verbatim

e0

“Over 96 % plant survival was recorded 15 days after transplantation”

✓ located verbatim in the source · abstract, characters 851–918 · show in text ↑
t0

“Sugarcane Variety CoS 17231”

✓ located verbatim in the source · title, characters 58–85 · show in text ↑

1-naphthaleneacetic acid · growth · Saccharum officinarum (sugarcane)Results differ

45 papers: 44 positive, 1 negative or no effect

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sucrose · growth · Saccharum officinarum (sugarcane)Positive and negative results

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38 papers: 36 positive, 2 negative or no effect

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  1. negative
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kinetin · growth · Saccharum officinarum (sugarcane)Results agree

12 papers: 12 positive, 0 negative or no effect

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  1. positive
  2. positive
  3. positive

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in vitro · growth · Saccharum officinarum (sugarcane)Positive and negative results

67 papers: 59 positive, 8 negative or no effect

58 papers in the same direction as this paper · 8 in the opposite direction

  1. positive
  2. negative
  3. negative

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.