Abstract
Plant-beneficial Pseudomonas bacteria hold the potential to be used as inoculants in agriculture to promote plant growth and health through various mechanisms. The discovery of new strains tailored to specific agricultural needs remains an open area of research. In this study, we report the isolation and characterization of four novel Pseudomonas species associated with the wheat rhizosphere. Comparative genomic analysis with all available Pseudomonas type strains revealed species-level differences, substantiated by both digital DNA-DNA hybridization and average nucleotide identity, underscoring their status as novel species. This was further validated by the phenotypic differences observed when compared to their closest relatives. Three of the novel species belong to the P. fluorescens species complex, with two representing a novel lineage in the Pseudomonas phylogeny. Functional genome annotation revealed the presence of specific features contributing to rhizosphere colonization, including flagella and components for biofilm formation. The novel species have the genetic potential to solubilize nutrients by acidifying the environment, releasing alkaline phosphatases and their metabolism of nitrogen species, indicating potential as biofertilizers. Additionally, the novel species possess traits that may facilitate direct promotion of plant growth through the modulation of the plant hormone balance, including the ACC deaminase enzyme and auxin metabolism. The presence of biosynthetic clusters for toxins such as hydrogen cyanide and non-ribosomal peptides suggests their ability to compete with other microorganisms, including plant pathogens. Direct inoculation of wheat roots significantly enhanced plant growth, with two strains doubling shoot biomass. Three of the strains effectively antagonized fungal phytopathogens ( Thielaviopsis basicola , Fusarium oxysporum , and Botrytis cinerea ), demonstrating their potential as biocontrol agents. Based on the observed genetic and phenotypic differences from closely related species, we propose the following names for the four novel species: Pseudomonas grandcourensis sp. nov., type strain DGS24 T ( = DSM 117501 T = CECT 31011 T ), Pseudomonas purpurea sp. nov., type strain DGS26 T ( = DSM 117502 T = CECT 31012 T ), Pseudomonas helvetica sp. nov., type strain DGS28 T ( = DSM 117503 T = CECT 31013 T ) and Pseudomonas aestiva sp. nov., type strain DGS32 T ( = DSM 117504 T = CECT 31014 T ).
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Claims 14
Claim · model-written summary
Four novel Pseudomonas species were isolated from the wheat rhizosphere.
- Taxon
- wheat
Triticum aestivume0 - Interactor
- Pseudomonas
Pseudomonase0symbiont - Outcome
- four novel Pseudomonas species associated with the wheat rhizospheree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four novel Pseudomonas species associated with the wheat rhizosphere.”
Claim · model-written summary
Comparative genomic analysis supported species-level differences from Pseudomonas type strains.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- PseudomonasPseudomonase0symbiont
- Outcome
- species-level differencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparative genomic analysis with all available Pseudomonas type strains revealed species-level differences, substantiated by both digital DNA-DNA hybridization and average nucleotide identity”
t0“wheat”
Claim · model-written summary
Phenotypic differences from closest relatives further supported the novel species.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- phenotypic differencese0
- Comparator
- closest relativese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This was further validated by the phenotypic differences observed when compared to their closest relatives.”
t0“wheat”
Claim · model-written summary
Three novel species belong to the P. fluorescens complex, including two in a new phylogenetic lineage.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- P. fluorescens
.JPG?width=60)
Pseudomonas fluorescense0symbiont - Outcome
- P. fluorescens species complexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.JPG?width=500)
Verbatim
e0“Three of the novel species belong to the P. fluorescens species complex, with two representing a novel lineage in the Pseudomonas phylogeny.”
t0“wheat”
Claim · model-written summary
Genome annotation identified flagella and biofilm components associated with rhizosphere colonization.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- features contributing to rhizosphere colonization, including flagella and components for biofilm formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional genome annotation revealed the presence of specific features contributing to rhizosphere colonization, including flagella and components for biofilm formation.”
t0“wheat”
Claim · model-written summary
Genomes suggested potential nutrient solubilization by acidification, alkaline phosphatases and nitrogen metabolism.
- Taxon
- wheatTriticum aestivumt0
- Gene
- alkaline phosphatasese0
- Outcome
- genetic potential to solubilize nutrientse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The novel species have the genetic potential to solubilize nutrients by acidifying the environment, releasing alkaline phosphatases and their metabolism of nitrogen species”
t0“wheat”
Claim · model-written summary
ACC deaminase and auxin metabolism traits suggested possible direct plant growth promotion.
- Taxon
- wheatTriticum aestivumt0
- Gene
- ACC deaminasee0
- Outcome
- direct promotion of plant growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“traits that may facilitate direct promotion of plant growth through the modulation of the plant hormone balance, including the ACC deaminase enzyme and auxin metabolism.”
t0“wheat”
Claim · model-written summary
Toxin biosynthetic clusters suggested ability to compete with other microorganisms, including plant pathogens.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- ability to compete with other microorganisms, including plant pathogense0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of biosynthetic clusters for toxins such as hydrogen cyanide and non-ribosomal peptides suggests their ability to compete with other microorganisms, including plant pathogens.”
t0“wheat”
Claim · model-written summary
Direct Pseudomonas inoculation significantly enhanced wheat growth.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Direct inoculatione0
- Interactor
- wheat rootsTriticum aestivume0symbiont
- Outcome
- plant growthe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Direct inoculation of wheat roots significantly enhanced plant growth”
Claim · model-written summary
Two inoculated strains doubled wheat shoot biomass.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- two strainse0
- Outcome
- shoot biomasse0
- Direction
- doublinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with two strains doubling shoot biomass.”
t0“wheat”
Claim · model-written summary
Three strains antagonized Thielaviopsis basicola.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- Thielaviopsis basicola
Berkeleyomyces basicolae0pathogen - Outcome
- antagonized fungal phytopathogense0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three of the strains effectively antagonized fungal phytopathogens ( Thielaviopsis basicola , Fusarium oxysporum , and Botrytis cinerea )”
t0“wheat”
Claim · model-written summary
Three strains antagonized Fusarium oxysporum.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- Fusarium oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume0pathogen - Outcome
- antagonized fungal phytopathogense0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e0“Three of the strains effectively antagonized fungal phytopathogens ( Thielaviopsis basicola , Fusarium oxysporum , and Botrytis cinerea )”
t0“wheat”
Claim · model-written summary
Three strains antagonized Botrytis cinerea.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Outcome
- antagonized fungal phytopathogense0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three of the strains effectively antagonized fungal phytopathogens ( Thielaviopsis basicola , Fusarium oxysporum , and Botrytis cinerea )”
t0“wheat”
Claim · model-written summary
Authors proposed four names for the novel Pseudomonas species.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- four novel speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we propose the following names for the four novel species: Pseudomonas grandcourensis sp. nov., type strain DGS24 T ( = DSM 117501 T = CECT 31011 T ), Pseudomonas purpurea sp. nov., type strain DGS26 T ( = DSM 117502 T = CECT 31012 T ), Pseudomonas helvetica sp. nov., type strain DGS28 T ( = DSM 117503 T = CECT 31013 T ) and Pseudomonas aestiva sp. nov., type strain DGS32 T ( = DSM 117504 T = CECT 31014 T ).”
t0“wheat”
The same questions in other papers
inoculation · growth · Triticum aestivum (wheat)Positive and negative results
475 papers in the same direction as this paper · 55 in the opposite direction · 1 reporting no difference from an alternative
- positive
- no effect
- no effect
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