Paper · 2024 · Frontiers in Microbiology

Antagonistic activity of two Bacillus strains against Fusarium oxysporum f. sp. capsici (FOC-1) causing Fusarium wilt and growth promotion activity of chili plant

Owais Iqbal; Rehana Naz Syed; Nasir Ahmed Rajput; et al.

11
claims
16
verbatim quotes
12
citations
2
research areas
✓
Open access · cc-by

Abstract

Fusarium oxysporum f. sp. capsici (Foc) poses a significant position in agriculture that has a negative impact on chili plant in terms of growth, fruit quality, and yield. Biological control is one of the promising strategies to control this pathogen in crops. Chili is considered as one of the most important crops in the Hyderabad region that is affected by Fusarium wilt disease. The pathogen was isolated from the infected samples in the region and was confirmed by morphological characteristics and PCR with a band of 488 bp. The bacterial strains were isolated from the rhizosphere soil of healthy plant and also confirmed by PCR with a band of 1,542 bp.The molecular characterization of the fungal and bacterial strain has shown 99.9% homology with the retrieved sequences of Fusarium oxysporum f. sp. capsici and Bacillus subtilis from NCBI. The 1-month-old Ghotki chili plants were inoculated with 1×10 5 cfu spore/ml −1 suspension and confirmed that the FOC -1 is responsible for chili Fusarium wilt disease. Subsequently, among the 33 screened Bacillus strains, only 11 showed antagonistic activity against F. oxysporum . Out of these, only two strains (AM13 and AM21) have shown maximum antagonistic activity against the pathogen by reducing the infection and promoting growth parameters of chili plants under both in vitro and greenhouse conditions. The study suggested that biological control is the most promising control strategy for the management of Fusarium wilt of chili in the field.

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Claims 11

Observationobservedoriginal resultmeasured

Claim · model-written summary

Fusarium pathogen identity in infected chili samples was confirmed by morphology and PCR.

Taxon
chilit0
Interactor
Fusarium oxysporum f. sp. capsici (FOC-1)Fusarium oxysporum f. sp. capsicit0pathogene0
Outcome
pathogen was isolatede0
Value
488 bpe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fusarium oxysporum f. sp. capsici
Fusarium oxysporum f. sp. capsici · Interactor · picture of its genus (no picture of exactly this name)Public domain · Wikimedia Commons ↗

Verbatim

e0

“The pathogen was isolated from the infected samples in the region and was confirmed by morphological characteristics and PCR with a band of 488 bp.”

✓ located verbatim in the source · abstract, characters 383–530 · show in text ↑
t0

“Fusarium oxysporum f. sp. capsici (FOC-1) causing Fusarium wilt and growth promotion activity of chili plant”

✓ located verbatim in the source · title, characters 54–162 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Rhizosphere bacterial strains were isolated from healthy plants and confirmed by PCR.

Taxon
chilit0
Outcome
bacterial strains were isolatede0
Value
1,542 bpe0

Verbatim

e0

“The bacterial strains were isolated from the rhizosphere soil of healthy plant and also confirmed by PCR with a band of 1,542 bp.”

✓ located verbatim in the source · abstract, characters 531–660 · show in text ↑
t0

“chili plant”

✓ located verbatim in the source · title, characters 151–162 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Molecular characterization showed 99.9% homology to Fusarium oxysporum f. sp. capsici reference sequences.

Taxon
chilit0
Interactor
Fusarium oxysporum f. sp. capsiciFusarium oxysporum f. sp. capsicie0pathogen
Outcome
homologye0
Value
99.9%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fusarium oxysporum f. sp. capsici
Fusarium oxysporum f. sp. capsici · Interactor · picture of its genus (no picture of exactly this name)Public domain · Wikimedia Commons ↗

Verbatim

e0

“The molecular characterization of the fungal and bacterial strain has shown 99.9% homology with the retrieved sequences of Fusarium oxysporum f. sp. capsici and Bacillus subtilis from NCBI.”

✓ located verbatim in the source · abstract, characters 660–849 · show in text ↑
t0

“chili plant”

✓ located verbatim in the source · title, characters 151–162 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Molecular characterization showed 99.9% homology to Bacillus subtilis reference sequences.

Taxon
chilit0
Interactor
Bacillus subtilisBacillus subtilise0biocontrol_agent
Outcome
homologye0
Value
99.9%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Bacillus subtilis
Bacillus subtilis · InteractorY tambe (original uploader) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The molecular characterization of the fungal and bacterial strain has shown 99.9% homology with the retrieved sequences of Fusarium oxysporum f. sp. capsici and Bacillus subtilis from NCBI.”

✓ located verbatim in the source · abstract, characters 660–849 · show in text ↑
t0

“chili plant”

✓ located verbatim in the source · title, characters 151–162 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Inoculation confirmed that FOC -1 causes Fusarium wilt in Ghotki chili.

Taxon
chilie0
Material
Ghotkie0
Treatment
inoculated with 1×10 5 cfu spore/ml −1 suspensione0
Interactor
FOC -1e0pathogen
Outcome
chili Fusarium wilt diseasee0

Not reportedComparatorDirection

Verbatim

e0

“The 1-month-old Ghotki chili plants were inoculated with 1×10 5 cfu spore/ml −1 suspension and confirmed that the FOC -1 is responsible for chili Fusarium wilt disease.”

✓ located verbatim in the source · abstract, characters 850–1018 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Eleven of 33 Bacillus strains showed antagonism against F. oxysporum.

Taxon
chilit0
Interactor
F. oxysporumFusarium oxysporume0pathogen
Second interactor
Bacilluse0biocontrol_agent
Outcome
antagonistic activity against F. oxysporume0
Value
11e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Subsequently, among the 33 screened Bacillus strains, only 11 showed antagonistic activity against F. oxysporum .”

✓ located verbatim in the source · abstract, characters 1019–1132 · show in text ↑
t0

“chili plant”

✓ located verbatim in the source · title, characters 151–162 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

AM13 reduced chili infection under in vitro and greenhouse conditions.

Taxon
chilie0
Treatment
AM13e0
Interactor
pathogene0pathogen
Second interactor
AM13e0biocontrol_agent
Outcome
infectione0
Direction
reducinge0
Setting
greenhouse conditionse0

Not reportedMaterialComparator

Verbatim

e0

“Out of these, only two strains (AM13 and AM21) have shown maximum antagonistic activity against the pathogen by reducing the infection and promoting growth parameters of chili plants under both in vitro and greenhouse conditions.”

✓ located verbatim in the source · abstract, characters 1133–1362 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

AM13 promoted chili growth parameters under in vitro and greenhouse conditions.

Taxon
chilie0
Treatment
AM13e0
Interactor
AM13e0biocontrol_agent
Outcome
growth parameters of chili plantse0
Direction
promotinge0
Setting
greenhouse conditionse0

Not reportedComparator

Verbatim

e0

“Out of these, only two strains (AM13 and AM21) have shown maximum antagonistic activity against the pathogen by reducing the infection and promoting growth parameters of chili plants under both in vitro and greenhouse conditions.”

✓ located verbatim in the source · abstract, characters 1133–1362 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

AM21 reduced chili infection under in vitro and greenhouse conditions.

Taxon
chilie0
Treatment
AM21e0
Interactor
pathogene0pathogen
Second interactor
AM21e0biocontrol_agent
Outcome
infectione0
Direction
reducinge0
Setting
greenhouse conditionse0

Not reportedMaterialComparator

Verbatim

e0

“Out of these, only two strains (AM13 and AM21) have shown maximum antagonistic activity against the pathogen by reducing the infection and promoting growth parameters of chili plants under both in vitro and greenhouse conditions.”

✓ located verbatim in the source · abstract, characters 1133–1362 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

AM21 promoted chili growth parameters under in vitro and greenhouse conditions.

Taxon
chilie0
Treatment
AM21e0
Interactor
AM21e0biocontrol_agent
Outcome
growth parameters of chili plantse0
Direction
promotinge0
Setting
greenhouse conditionse0

Not reportedComparator

Verbatim

e0

“Out of these, only two strains (AM13 and AM21) have shown maximum antagonistic activity against the pathogen by reducing the infection and promoting growth parameters of chili plants under both in vitro and greenhouse conditions.”

✓ located verbatim in the source · abstract, characters 1133–1362 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors recommend biological control for field management of chili Fusarium wilt.

Taxon
chilie0
Treatment
biological controle0
Outcome
management of Fusarium wilt of chilie0
Setting
fielde0

Verbatim

e0

“The study suggested that biological control is the most promising control strategy for the management of Fusarium wilt of chili in the field.”

✓ located verbatim in the source · abstract, characters 1363–1504 · show in text ↑

inoculation · disease · Capsicum (peppers)Positive and negative results

30 papers: 12 positive, 18 negative or no effect

17 papers in the same direction as this paper · 12 in the opposite direction

  1. positive
  2. positive
  3. positive

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.