Abstract
Phytophthora root and stem rot of soybean (Glycine max), caused by the oomycete Phytophthora sojae, is an extremely destructive disease worldwide. In this study, we identified GmEIL1, which encodes an ethylene-insensitive3 (EIN3) transcription factor. GmEIL1 was significantly induced following P. sojae infection of soybean plants. Compared to wild-type soybean plants, transgenic soybean plants overexpressing GmEIL1 showed enhanced resistance to P. sojae and GmEIL1-silenced RNA-interference lines showed more severe symptoms when infected with P. sojae. We screened for target genes of GmEIL1 and confirmed that GmEIL1 bound directly to the GmERF113 promoter and regulated GmERF113 expression. Moreover, GmEIL1 positively regulated the expression of the pathogenesis-related gene GmPR1. The GmEIL1-regulated defence response to P. sojae involved both ethylene biosynthesis and the ethylene signalling pathway. These findings suggest that the GmEIL1-GmERF113 module plays an important role in P. sojae resistance via the ethylene signalling pathway.
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Claims 8
Claim · model-written summary
GmEIL1 encodes an EIN3 transcription factor.
- Taxon
- soybean
Glycine maxt0 - Gene
- GmEIL1e0
- Outcome
- encodes an ethylene-insensitive3 (EIN3) transcription factore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmEIL1, which encodes an ethylene-insensitive3 (EIN3) transcription factor”
t0“soybean”
Claim · model-written summary
P. sojae infection significantly induced GmEIL1.
- Taxon
- soybeanGlycine maxe0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmEIL1e0
- Outcome
- GmEIL1e0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmEIL1 was significantly induced following P. sojae infection of soybean plants”
Claim · model-written summary
GmEIL1 overexpression enhanced soybean resistance to P. sojae relative to wild type.
- Taxon
- soybeanGlycine maxe0
- Treatment
- overexpressing GmEIL1e0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmEIL1e0
- Outcome
- resistance to P. sojaee0
- Direction
- enhancede0
- Comparator
- wild-type soybean plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to wild-type soybean plants, transgenic soybean plants overexpressing GmEIL1 showed enhanced resistance to P. sojae”
Claim · model-written summary
GmEIL1 silencing worsened soybean symptoms under P. sojae infection.
- Taxon
- soybeanGlycine maxt0
- Treatment
- GmEIL1-silenced RNA-interferencee0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmEIL1e0
- Outcome
- symptomse0
- Direction
- more severee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmEIL1-silenced RNA-interference lines showed more severe symptoms when infected with P. sojae”
t0“soybean”
Claim · model-written summary
GmEIL1 directly bound the GmERF113 promoter.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmEIL1e0
- Outcome
- bound directly to the GmERF113 promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmEIL1 bound directly to the GmERF113 promoter”
t0“soybean”
Claim · model-written summary
GmEIL1 regulated GmERF113 expression.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmEIL1e0
- Outcome
- GmERF113 expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmEIL1 bound directly to the GmERF113 promoter and regulated GmERF113 expression”
t0“soybean”
Claim · model-written summary
GmEIL1 positively regulated GmPR1 expression.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmEIL1e0
- Outcome
- expression of the pathogenesis-related gene GmPR1e0
- Direction
- positively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmEIL1 positively regulated the expression of the pathogenesis-related gene GmPR1”
t0“soybean”
Claim · model-written summary
GmEIL1-dependent defence involved ethylene biosynthesis and signalling.
- Taxon
- soybeanGlycine maxt0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmEIL1e0
- Outcome
- defence response to P. sojaee0
- Qualifier
- ethylene biosynthesis and the ethylene signalling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GmEIL1-regulated defence response to P. sojae involved both ethylene biosynthesis and the ethylene signalling pathway”
t0“soybean”
The same questions in other papers
loss of function · Phytophthora sojae · Glycine max (soybean)Positive and negative results
14 papers in the same direction as this paper · 9 in the opposite direction
- positive
- negative
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See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.