Abstract
Negative environmental impacts of chemical pesticides used for pest and disease management are an increasing issue. So, safe alternatives are being developed. One of the most effective methods is to use microbial biocontrol agents as antifungal biopesticides. The aim of this study was to explore the mode of action and evaluate the potentiality of the novel bio-fungicide T34 Biocontrol ® (Trichoderma asperellum strain T34) against strawberry fruit-rot-causing fungal pathogens. Different fungal plant pathogens were isolated from diseased fruits of strawberry showing rots symptoms, collected from a field (Royal Fruits Farm, fresh planting system) at Abo-Ghaleb Al-Mahatta, Abo-Ghaleb city region, Giza Gov., Egypt. Ten isolates were isolated and identified successfully. Acremonium butyri, Alternaria tenuissima, Fusarium oxysporum, F. sporotrichioides, F. subglutinans, Mucor hiemalis, Rhizoctonia fragaria, R. solani and Trichothecium roseum were associated with (as causal complex of) strawberry fruit rot diseases. The commercial formulation of T34 Biocontrol ® (Trichoderma asperellum strain, T34) was evaluated under lab conditions (in vitro) against these isolates throw two different techniques, growing on Trichoderma asperellum-mixed PDA medium plates and dual-culture. Trichoderma asperellum strain, T34 showed good antagonistic activities and significantly inhibited the fungal growth of all fungal pathogens tested. Also, the separation of chemical substances of partially purified culture filtrate of T. asperellum by GC-MS clarified that different chemical compounds with generally good antimicrobial, antibacterial, bactericidal, antifungal, antioxidant, anti-inflammatory, local anesthetic, antinociceptive, cicatrizing, antiseptic or anticancer properties can be produced by the tested biocontrol agent T34 and plays as antibiotics inhibiting the growth of plant pathogens. Separation of chemical substances of partially purified culture filtrate of T. asperellum by GCMS, showed that T. asperellum produces compounds (e.g., 9- Octadecenoic acid, Anthrone, Thymol, Glycerol, 1-Hexadecanol,2- methyl-, D-Mannitol, Palmitic Acid, Oleic Acid, Stearic acid, 1- Monopalmitin, Acetic acid, hydrazide, Citronellene, Carveol 1, Linalool, Stanozolol and Arachidonic acid) with antimicrobial activities according to available literature. In vivo assay of the biocontrol agent T34 Biocontrol ® under open field conditions against strawberry fruit rot diseases showed that the field application (spraying) of T34 significantly reduced the disease (fruit rots) incidence preharvest (on the field) and disease incidence and severity postharvest (during cooled storage) in both frequently tested winter seasons 2020-2021 and 2021-2022. T34 achieved highly efficient protective performance and led to a disease reduction percentage exceeding 90 % in some trials. Our results were nearly comparable with disease control achieved by comparative chemical fungicides of Fabolous 75%WP (Tebuconazole 12.5%+Chlorothalonil 62.5%(w/w)), Fabric 30%SC (Tebuconazole 15%+Kresoxim-Methyl 15%(w/v)), Fango 50%WG (Pyraclostrobin 50%(w/w)), Klop 50%WP (Difenoconazole 6%+Thiophanate methyl 44% (w/w)), Maven 18.7%WG (Pyraclostrobin 6.7%+Dimethomorph 12%(w/w)) and Mystic 20%WP (Pyrimethanil 20%(w/w)).
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Claims 8
Claim · model-written summary
Ten fungal isolates were identified from diseased strawberry fruits.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Outcome
- isolatese0
- Value
- Ten isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Ten isolates were isolated and identified successfully”
t0“Strawberry”
Claim · model-written summary
Nine named fungi were associated with strawberry fruit rot.
- Taxon
- strawberryFragaria ×ananassae0
- Interactor
- Acremonium butyriCosmospora butyrie0pathogen
- Outcome
- strawberry fruit rot diseasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acremonium butyri, Alternaria tenuissima, Fusarium oxysporum, F. sporotrichioides, F. subglutinans, Mucor hiemalis, Rhizoctonia fragaria, R. solani and Trichothecium roseum were associated with (as causal complex of) strawberry fruit rot diseases”
Claim · model-written summary
T34 significantly inhibited growth of tested strawberry fruit-rot fungi in vitro.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- Trichoderma asperellum strain, T34e0
- Interactor
- fungal pathogense0pathogen
- Second interactor
- Trichoderma asperellum strain, T34e0biocontrol_agent
- Outcome
- fungal growth of all fungal pathogens testede0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Trichoderma asperellum strain, T34 showed good antagonistic activities and significantly inhibited the fungal growth of all fungal pathogens tested”
t0“Strawberry”
Claim · model-written summary
T34 culture filtrate contained multiple compounds with reported antimicrobial activities.
- Taxon
- StrawberryFragaria ×ananassat0
- Interactor
- T. asperellum
Trichoderma asperellume0biocontrol_agent - Outcome
- compoundse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“T. asperellum produces compounds (e.g., 9- Octadecenoic acid, Anthrone, Thymol, Glycerol, 1-Hexadecanol,2- methyl-, D-Mannitol, Palmitic Acid, Oleic Acid, Stearic acid, 1- Monopalmitin, Acetic acid, hydrazide, Citronellene, Carveol 1, Linalool, Stanozolol and Arachidonic acid) with antimicrobial activities according to available literature”
t0“Strawberry”
Claim · model-written summary
Field spraying with T34 significantly reduced preharvest strawberry fruit-rot incidence.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- field application (spraying) of T34e0
- Interactor
- T34e0biocontrol_agent
- Outcome
- disease (fruit rots) incidence preharveste0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“field application (spraying) of T34 significantly reduced the disease (fruit rots) incidence preharvest (on the field)”
t0“Strawberry”
Claim · model-written summary
Field spraying with T34 significantly reduced postharvest fruit-rot incidence and severity.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- field application (spraying) of T34e0
- Interactor
- T34e0biocontrol_agent
- Outcome
- disease incidence and severity postharveste0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“field application (spraying) of T34 significantly reduced the disease (fruit rots) incidence preharvest (on the field) and disease incidence and severity postharvest (during cooled storage)”
t0“Strawberry”
Claim · model-written summary
T34 reduced disease by over 90% in some trials.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- T34e0
- Interactor
- T34e0biocontrol_agent
- Outcome
- disease reduction percentagee0
- Direction
- reductione0
- Value
- 90 %e0
- Qualifier
- in some trialse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“T34 achieved highly efficient protective performance and led to a disease reduction percentage exceeding 90 % in some trials”
t0“Strawberry”
Claim · model-written summary
T34 disease control was nearly comparable with chemical fungicides.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- T34t0
- Interactor
- T34t0biocontrol_agent
- Outcome
- disease controle0
- Direction
- nearly comparablee0
- Comparator
- chemical fungicidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results were nearly comparable with disease control achieved by comparative chemical fungicides of Fabolous 75%WP (Tebuconazole 12.5%+Chlorothalonil 62.5%(w/w)), Fabric 30%SC (Tebuconazole 15%+Kresoxim-Methyl 15%(w/v)), Fango 50%WG (Pyraclostrobin 50%(w/w)), Klop 50%WP (Difenoconazole 6%+Thiophanate methyl 44% (w/w)), Maven 18.7%WG (Pyraclostrobin 6.7%+Dimethomorph 12%(w/w)) and Mystic 20%WP (Pyrimethanil 20%(w/w))”
t0“T34 Biocontrol ® (Trichoderma asperellum strain, T34), a Biocontrol Agent Reducing Strawberry Fruit Rots”
The same questions in other papers
Trichoderma asperellum · disease · Fragaria x ananassa (strawberry)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
application · disease · Fragaria x ananassa (strawberry)Positive and negative results
34 papers in the same direction as this paper · 4 in the opposite direction · 1 reporting no difference from an alternative
- positive
- positive
- negative
36 papers more in this group
See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.