Abstract
Plant surface treatment with double-stranded RNAs (dsRNAs) has gained recognition as a promising method for inducing gene silencing and combating plant pathogens. However, the regulation of endogenous plant genes by external dsRNAs has not been sufficiently investigated. Also, the effect of the simultaneous application of multiple gene-specific dsRNAs has not been analyzed. The aim of this study was to exogenously target five genes in Arabidopsis thaliana, namely, three transcription factor genes (AtCPC, AtMybL2, AtANAC032), a calmodulin-binding protein gene (AtCBP60g), and an anthocyanidin reductase gene (AtBAN), which are known as negative regulators of anthocyanin accumulation. Exogenous dsRNAs encoding these genes were applied to the leaf surface of A. thaliana either individually or in mixtures. The mRNA levels of the five targets were analyzed using qRT-PCR, and anthocyanin content was evaluated through HPLC-MS. The results demonstrated significant downregulation of all five target genes by the exogenous dsRNAs, resulting in enhanced expression of chalcone synthase (AtCHS) gene and increased anthocyanin content. The simultaneous foliar application of the five dsRNAs proved to be more efficient in activating anthocyanin accumulation compared to the application of individual dsRNAs. These findings hold considerable importance in plant biotechnology and gene function studies.
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Claims 4
Claim · model-written summary
Exogenous dsRNAs significantly downregulated all five target genes in Arabidopsis thaliana
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- exogenous dsRNAse1
- Outcome
- all five target genese1
- Direction
- downregulatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The aim of this study was to exogenously target five genes in Arabidopsis thaliana”
e1“The results demonstrated significant downregulation of all five target genes by the exogenous dsRNAs”
Claim · model-written summary
Exogenous dsRNA application enhanced expression of the chalcone synthase (AtCHS) gene in Arabidopsis thaliana
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Exogenous dsRNAse1
- Gene
- AtCHSe2
- Outcome
- expression of chalcone synthase (AtCHS) genee2
- Direction
- enhancede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The aim of this study was to exogenously target five genes in Arabidopsis thaliana”
e1“Exogenous dsRNAs encoding these genes were applied to the leaf surface”
e2“resulting in enhanced expression of chalcone synthase (AtCHS) gene and increased anthocyanin content”
Claim · model-written summary
Exogenous dsRNA application increased anthocyanin content in Arabidopsis thaliana
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Exogenous dsRNAse1
- Outcome
- anthocyanin contente2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The aim of this study was to exogenously target five genes in Arabidopsis thaliana”
e1“Exogenous dsRNAs encoding these genes were applied to the leaf surface”
e2“resulting in enhanced expression of chalcone synthase (AtCHS) gene and increased anthocyanin content”
Claim · model-written summary
Simultaneous foliar application of the five dsRNAs increased anthocyanin accumulation more efficiently than individual dsRNAs
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- simultaneous foliar application of the five dsRNAse3
- Outcome
- anthocyanin accumulatione3
- Direction
- more efficiente3
- Comparator
- application of individual dsRNAse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The aim of this study was to exogenously target five genes in Arabidopsis thaliana”
e3“The simultaneous foliar application of the five dsRNAs proved to be more efficient in activating anthocyanin accumulation compared to the application of individual dsRNAs”
The same questions in other papers
double-stranded RNA · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
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1 paper more in this group
See all 5 papers →double-stranded RNA · quality: phytochemicals · Arabidopsis thaliana (thale cress)Results agree
1 paper in the same direction as this paper
- positive
application · quality: phytochemicals · Arabidopsis thaliana (thale cress)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.