Abstract
Subclass III sucrose nonfermenting1-related protein kinase 2s (SnRK2s) function in ABA and abiotic stress responses by unknown mechanisms. We found that osmotic stress/ABA-activated protein kinase 10 (SAPK10), a member of rice SnRK2s, physically interacted with CBL-interacting protein kinase 1 (OsCIPK1). OsCIPK1 expression was up-regulated by ABA and PEG treatment, and overexpression increased the ABA sensitivity of seed germination and root growth and plant osmotic stress tolerance. Osmotic stress or ABA-induced activation of OsCIPK1 is dependent on SAPK10. SAPK10 phosphorylates Thr-24 of OsCIPK1 in vitro, and this phosphorylation increases the activity of OsCIPK1 and positively regulates the function of OsCIPK1 in ABA responses and plant osmotic stress tolerance. This study suggests that OsCIPK1 is a direct phosphorylated substrate of SAPK10, and SAPK10-mediated phosphorylation of OsCIPK1 functions in ABA signaling and increases rice osmotic stress tolerance.
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Claims 10
Claim · model-written summary
SAPK10 physically interacted with OsCIPK1 in rice.
- Taxon
- rice
Oryza sativae0 - Gene
- SAPK10e0
- Outcome
- physically interacted with CBL-interacting protein kinase 1 (OsCIPK1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in rice”
e0“SAPK10), a member of rice SnRK2s, physically interacted with CBL-interacting protein kinase 1 (OsCIPK1)”
Claim · model-written summary
ABA up-regulated OsCIPK1 expression.
- Taxon
- riceOryza sativat0
- Treatment
- ABAe0
- Gene
- OsCIPK1e0
- Outcome
- OsCIPK1 expressione0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in rice”
e0“OsCIPK1 expression was up-regulated by ABA and PEG treatment”
Claim · model-written summary
PEG treatment up-regulated OsCIPK1 expression.
- Taxon
- riceOryza sativat0
- Treatment
- PEG treatmente0
- Gene
- OsCIPK1e0
- Outcome
- OsCIPK1 expressione0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in rice”
e0“OsCIPK1 expression was up-regulated by ABA and PEG treatment”
Claim · model-written summary
OsCIPK1 overexpression increased ABA sensitivity of seed germination and root growth.
- Taxon
- riceOryza sativat0
- Treatment
- overexpressione0
- Outcome
- ABA sensitivity of seed germination and root growthe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in rice”
e0“overexpression increased the ABA sensitivity of seed germination and root growth”
Claim · model-written summary
OsCIPK1 overexpression increased plant osmotic stress tolerance.
- Taxon
- riceOryza sativat0
- Treatment
- overexpressione0
- Outcome
- plant osmotic stress tolerancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in rice”
e0“overexpression increased the ABA sensitivity of seed germination and root growth and plant osmotic stress tolerance”
Claim · model-written summary
Osmotic stress or ABA activation of OsCIPK1 depends on SAPK10.
- Taxon
- riceOryza sativat0
- Gene
- SAPK10e0
- Outcome
- activation of OsCIPK1e0
- Qualifier
- dependent on SAPK10e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in rice”
e0“Osmotic stress or ABA-induced activation of OsCIPK1 is dependent on SAPK10”
Claim · model-written summary
SAPK10 phosphorylates Thr-24 of OsCIPK1 in vitro.
- Taxon
- riceOryza sativat0
- Gene
- SAPK10e0
- Outcome
- phosphorylates Thr-24 of OsCIPK1e0
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in rice”
e0“SAPK10 phosphorylates Thr-24 of OsCIPK1 in vitro”
Claim · model-written summary
Phosphorylation by SAPK10 increases OsCIPK1 activity.
- Taxon
- riceOryza sativat0
- Gene
- OsCIPK1e0
- Outcome
- activity of OsCIPK1e0
- Direction
- increasese0
- Qualifier
- this phosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in rice”
e0“this phosphorylation increases the activity of OsCIPK1”
Claim · model-written summary
Phosphorylation positively regulates OsCIPK1 function in ABA responses.
- Taxon
- riceOryza sativat0
- Gene
- OsCIPK1e0
- Outcome
- function of OsCIPK1 in ABA responsese0
- Direction
- positively regulatese0
- Qualifier
- this phosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in rice”
e0“this phosphorylation increases the activity of OsCIPK1 and positively regulates the function of OsCIPK1 in ABA responses and plant osmotic stress tolerance”
Claim · model-written summary
Phosphorylation positively regulates OsCIPK1 function in plant osmotic stress tolerance.
- Taxon
- riceOryza sativat0
- Gene
- OsCIPK1e0
- Outcome
- plant osmotic stress tolerancee0
- Direction
- positively regulatese0
- Qualifier
- this phosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in rice”
e0“this phosphorylation increases the activity of OsCIPK1 and positively regulates the function of OsCIPK1 in ABA responses and plant osmotic stress tolerance”
The same questions in other papers
(+)-abscisic acid · quality: protein · Oryza sativa (rice)Positive and negative results
3 papers in the same direction as this paper · 3 in the opposite direction
- no effect
- negative
- no effect
3 papers more in this group
See all 7 papers →drought · quality: protein · Oryza sativa (rice)Results differ
6 papers in the same direction as this paper · 8 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.