Abstract
Introduction: Heavy ion beam is a novel approach for crop mutagenesis with the advantage of high energy transfer line density and low repair effect after injury, however, little investigation on the biological effect on plant was performed. 50 Gy irradiation significantly stimulated the growth of Arabidopsis seedlings, as indicated by an increase in root and biomass, while 200 Gy irradiation significantly inhibited the growth of seedlings, causing a visible decrease in plant growth. Methods: The Arabidopsis seeds were irradiated by 12C6+. Monte Carlo simulations were used to calculate the damage to seeds and particle trajectories by ion implantation. The seed epidermis received SEM detection and changes in its organic composition were detected using FTIR. Evidence of ROS and antioxidant systems were analyzed. RNA-seq and qPCR were used to detect changes in seedling transcript levels. Results and discussion: Monte Carlo simulations revealed that high-dose irradiation causes various damage. Evidence of ROS and antioxidant systems implies that the emergence of phenotypes in plant cells may be associated with oxidative stress. Transcriptomic analysis of the seedlings demonstrated that 170 DEGs were present in the 50 Gy and 200 Gy groups and GO enrichment indicated that they were mainly associated with stress resistance and cell wall homeostasis. Further GO enrichment of DEGs unique to 50 Gy and 200 Gy revealed 58 50Gy-exclusive DEGs were enriched in response to oxidative stress and jasmonic acid entries, while 435 200 Gy-exclusive DEGs were enriched in relation to oxidative stress, organic cyclic compounds, and salicylic acid. This investigation advances our insight into the biological effects of heavy ion irradiation and the underlying mechanisms.
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Claims 8
Claim · model-written summary
50 Gy irradiation stimulated seedling root growth and biomass.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- 50 Gy irradiatione0
- Outcome
- root and biomasse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“50 Gy irradiation significantly stimulated the growth of Arabidopsis seedlings, as indicated by an increase in root and biomass”
Claim · model-written summary
200 Gy irradiation inhibited seedling growth.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- 200 Gy irradiatione0
- Outcome
- plant growthe0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“200 Gy irradiation significantly inhibited the growth of seedlings, causing a visible decrease in plant growth”
t0“Arabidopsis”
Claim · model-written summary
Simulation indicated varied damage from high dose irradiation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- high-dose irradiatione0
- Outcome
- various damagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Monte Carlo simulations revealed that high-dose irradiation causes various damage”
t0“Arabidopsis”
Claim · model-written summary
Oxidative stress may be associated with the irradiated cell phenotypes.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- oxidative stresse0
- Outcome
- emergence of phenotypes in plant cellse0
- Qualifier
- oxidative stresse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the emergence of phenotypes in plant cells may be associated with oxidative stress”
t0“Arabidopsis”
Claim · model-written summary
The two irradiation groups had 170 differentially expressed genes.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- 50 Gy and 200 Gye0
- Outcome
- DEGse0
- Value
- 170e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“170 DEGs were present in the 50 Gy and 200 Gy groups”
t0“Arabidopsis”
Claim · model-written summary
Common differentially expressed genes were enriched for stress resistance and cell wall homeostasis.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- stress resistance and cell wall homeostasise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they were mainly associated with stress resistance and cell wall homeostasis”
t0“Arabidopsis”
Claim · model-written summary
The 50 Gy group had 58 exclusive DEGs enriched for oxidative stress and jasmonic acid response.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- 50Gye0
- Outcome
- DEGs were enriched in response to oxidative stress and jasmonic acid entriese0
- Value
- 58e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“58 50Gy-exclusive DEGs were enriched in response to oxidative stress and jasmonic acid entries”
t0“Arabidopsis”
Claim · model-written summary
The 200 Gy group had 435 exclusive DEGs enriched for oxidative stress, organic cyclic compounds and salicylic acid.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- 200 Gye0
- Outcome
- DEGs were enriched in relation to oxidative stress, organic cyclic compounds, and salicylic acide0
- Value
- 435e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“435 200 Gy-exclusive DEGs were enriched in relation to oxidative stress, organic cyclic compounds, and salicylic acid”
t0“Arabidopsis”
The same questions in other papers
radiation · growth · ArabidopsisPositive and negative results
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.