Abstract
Rice is a dominant source of inorganic arsenic (As) exposure for populations consuming rice as a staple food. Decreasing As accumulation in rice grain is important for improving food safety. Arsenite [As(III)], the main form of As in paddy soil porewater, is taken up inadvertently by OsLsi1 and OsLsi2, the two key transporters for silicon (Si) uptake in rice roots. Here, we investigated whether editing OsLsi1 or OsLsi2 can decrease As accumulation in rice grain without compromising grain yield. We used the CRISPR-Cas9 technology to edit the promoter region of OsLsi1 and the C-terminal coding sequence of OsLsi1 and OsLsi2, and we generated a total of 27 mutants. Uptake and accumulation of Si and As were evaluated in both short-term hydroponic experiments and in a paddy field. Deletion of 1.2–2 kb of the OsLsi1 promoter suppressed OsLsi1 expression in roots and Si uptake markedly and did not affect As(III) uptake or grain As concentration. Some of the OsLsi1 and OsLsi2 coding sequence mutants showed large decreases in the uptake of Si and As(III) as well as large decreases in Si accumulation in rice husks. However, only OsLsi2 mutants showed significant decreases (by up to 63%) in the grain total As concentration. Editing OsLsi2 mainly affected the accumulation of inorganic As in rice grain with little effect on the accumulation of dimethylarsenate (DMA). Grain yields of the OsLsi2 mutants were comparable to those of the wild type. Editing OsLsi2 provides a promising way to reduce As accumulation in rice grain without compromising the grain yield.
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Claims 12
Claim · model-written summary
Deleting the OsLsi1 promoter suppressed root OsLsi1 expression.
- Taxon
- Rice
Oryza sativat0 - Treatment
- Deletion of 1.2–2 kb of the OsLsi1 promotere0
- Gene
- OsLsi1e0
- Outcome
- OsLsi1 expression in rootse0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletion of 1.2–2 kb of the OsLsi1 promoter suppressed OsLsi1 expression in roots and Si uptake markedly and did not affect As(III) uptake or grain As concentration.”
t0“Rice”
Claim · model-written summary
Deleting the OsLsi1 promoter markedly suppressed Si uptake.
- Taxon
- RiceOryza sativat0
- Treatment
- Deletion of 1.2–2 kb of the OsLsi1 promotere0
- Gene
- OsLsi1e0
- Outcome
- Si uptakee0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletion of 1.2–2 kb of the OsLsi1 promoter suppressed OsLsi1 expression in roots and Si uptake markedly and did not affect As(III) uptake or grain As concentration.”
t0“Rice”
Claim · model-written summary
Deleting the OsLsi1 promoter did not affect As(III) uptake.
- Taxon
- RiceOryza sativat0
- Treatment
- Deletion of 1.2–2 kb of the OsLsi1 promotere0
- Gene
- OsLsi1e0
- Outcome
- As(III) uptakee0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletion of 1.2–2 kb of the OsLsi1 promoter suppressed OsLsi1 expression in roots and Si uptake markedly and did not affect As(III) uptake or grain As concentration.”
t0“Rice”
Claim · model-written summary
Deleting the OsLsi1 promoter did not affect grain As concentration.
- Taxon
- RiceOryza sativat0
- Treatment
- Deletion of 1.2–2 kb of the OsLsi1 promotere0
- Gene
- OsLsi1e0
- Outcome
- grain As concentratione0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletion of 1.2–2 kb of the OsLsi1 promoter suppressed OsLsi1 expression in roots and Si uptake markedly and did not affect As(III) uptake or grain As concentration.”
t0“Rice”
Claim · model-written summary
Some OsLsi1 coding sequence mutants had greatly reduced Si uptake.
- Taxon
- RiceOryza sativat0
- Material
- OsLsi1e0
- Treatment
- OsLsi1 and OsLsi2 coding sequence mutantse0
- Gene
- OsLsi1e0
- Outcome
- uptake of Sie0
- Direction
- large decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some of the OsLsi1 and OsLsi2 coding sequence mutants showed large decreases in the uptake of Si and As(III) as well as large decreases in Si accumulation in rice husks.”
t0“Rice”
Claim · model-written summary
Some OsLsi2 coding sequence mutants had greatly reduced As(III) uptake.
- Taxon
- RiceOryza sativat0
- Treatment
- OsLsi1 and OsLsi2 coding sequence mutantse0
- Gene
- OsLsi2e0
- Outcome
- uptake of Si and As(III)e0
- Direction
- large decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some of the OsLsi1 and OsLsi2 coding sequence mutants showed large decreases in the uptake of Si and As(III) as well as large decreases in Si accumulation in rice husks.”
t0“Rice”
Claim · model-written summary
Some OsLsi1 and OsLsi2 mutants had greatly reduced Si accumulation in rice husks.
- Taxon
- riceOryza sativae0
- Treatment
- OsLsi1 and OsLsi2 coding sequence mutantse0
- Gene
- OsLsi1 and OsLsi2e0
- Outcome
- Si accumulation in rice huskse0
- Direction
- large decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some of the OsLsi1 and OsLsi2 coding sequence mutants showed large decreases in the uptake of Si and As(III) as well as large decreases in Si accumulation in rice husks.”
Claim · model-written summary
Only OsLsi2 mutants significantly reduced total grain As concentration, by up to 63%.
- Taxon
- RiceOryza sativat0
- Treatment
- OsLsi2 mutantse0
- Gene
- OsLsi2e0
- Outcome
- grain total As concentratione0
- Direction
- decreasese0
- Value
- up to 63%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only OsLsi2 mutants showed significant decreases (by up to 63%) in the grain total As concentration.”
t0“Rice”
Claim · model-written summary
OsLsi2 editing mainly affected inorganic As accumulation in rice grain.
- Taxon
- riceOryza sativae0
- Treatment
- Editing OsLsi2e0
- Gene
- OsLsi2e0
- Outcome
- accumulation of inorganic As in rice graine0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Editing OsLsi2 mainly affected the accumulation of inorganic As in rice grain with little effect on the accumulation of dimethylarsenate (DMA).”
Claim · model-written summary
OsLsi2 editing had little effect on dimethylarsenate accumulation.
- Taxon
- riceOryza sativae0
- Treatment
- Editing OsLsi2e0
- Gene
- OsLsi2e0
- Outcome
- accumulation of dimethylarsenate (DMA)e0
- Direction
- little effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Editing OsLsi2 mainly affected the accumulation of inorganic As in rice grain with little effect on the accumulation of dimethylarsenate (DMA).”
Claim · model-written summary
Grain yields of OsLsi2 mutants were comparable to wild type.
- Taxon
- RiceOryza sativat0
- Treatment
- OsLsi2 mutantse0
- Gene
- OsLsi2e0
- Outcome
- Grain yieldse0
- Direction
- comparablee0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Grain yields of the OsLsi2 mutants were comparable to those of the wild type.”
t0“Rice”
Claim · model-written summary
The authors consider OsLsi2 editing promising for reducing grain As without losing yield.
- Taxon
- riceOryza sativae0
- Gene
- OsLsi2e0
- Outcome
- Editing OsLsi2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Editing OsLsi2 provides a promising way to reduce As accumulation in rice grain without compromising the grain yield.”
The same questions in other papers
loss of function · nutrient: content · Oryza sativa (rice)Positive and negative results
35 papers in the same direction as this paper · 20 in the opposite direction
- positive
- no effect
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See all 56 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.