Abstract
Our previous study showed that COPPER-CONTAINING AMINE OXIDASE (CuAO) and AMINOALDEHYDE DEHYDROGENASE (AMADH) could regulate the accumulation of γ-aminobutyric acid (GABA) in tea through the polyamine degradation pathway. However, their biological function in drought tolerance has not been determined. In this study, Camellia sinensis (Cs) CsCuAO1 associated with CsAMADH1 conferred drought tolerance, which modulated GABA levels in tea plants. The results showed that exogenous GABA spraying effectively alleviated the drought-induced physical damage. Arabidopsis lines overexpressing CsCuAO1 and CsAMADH1 exhibited enhanced resistance to drought, which promoted the synthesis of GABA and putrescine by stimulating reactive oxygen species’ scavenging capacity and stomatal movement. However, the suppression of CsCuAO1 or CsAMADH1 in tea plants resulted in increased sensitivity to drought treatment. Moreover, co-overexpressing plants increased GABA accumulation both in an Agrobacterium-mediated Nicotiana benthamiana transient assay and transgenic Arabidopsis plants. In addition, a GABA transporter gene, CsGAT1, was identified, whose expression was strongly correlated with GABA accumulation levels in different tissues under drought stress. Taken together, CsCuAO1 and CsAMADH1 were involved in the response to drought stress through a dynamic GABA-putrescine balance. Our data will contribute to the characterization of GABA’s biological functions in response to environmental stresses in plants.
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Claims 11
Claim · model-written summary
CsCuAO1 associated with CsAMADH1 conferred drought tolerance and modulated GABA levels in tea.
Verbatim
e0“Camellia sinensis (Cs) CsCuAO1 associated with CsAMADH1 conferred drought tolerance, which modulated GABA levels in tea plants”
Claim · model-written summary
Exogenous GABA spraying alleviated drought-induced physical damage in tea.
- Taxon
- tea plantst0
- Treatment
- exogenous GABA sprayinge0
- Outcome
- drought-induced physical damagee0
- Direction
- alleviatede0
Not reportedComparator
Verbatim
e0“exogenous GABA spraying effectively alleviated the drought-induced physical damage”
t0“tea plants”
Claim · model-written summary
Arabidopsis lines overexpressing CsCuAO1 and CsAMADH1 had enhanced drought resistance.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- overexpressing CsCuAO1 and CsAMADH1e0
- Gene
- CsCuAO1 and CsAMADH1e0
- Outcome
- resistance to droughte0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis lines overexpressing CsCuAO1 and CsAMADH1 exhibited enhanced resistance to drought”
Claim · model-written summary
Overexpression promoted GABA and putrescine synthesis in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpressing CsCuAO1 and CsAMADH1e0
- Gene
- CsCuAO1 and CsAMADH1e0
- Outcome
- synthesis of GABA and putrescinee0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis lines overexpressing CsCuAO1 and CsAMADH1 exhibited enhanced resistance to drought, which promoted the synthesis of GABA and putrescine”
Claim · model-written summary
The authors attribute the enhanced drought response to reactive oxygen species scavenging and stomatal movement.
- Taxon
- ArabidopsisArabidopsise1
- Outcome
- reactive oxygen species’ scavenging capacity and stomatal movemente0
- Qualifier
- stimulatinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“by stimulating reactive oxygen species’ scavenging capacity and stomatal movement”
t0“tea plants”
e1“Arabidopsis lines overexpressing CsCuAO1 and CsAMADH1 exhibited enhanced resistance to drought”
Claim · model-written summary
Suppressing CsCuAO1 increased tea sensitivity to drought.
- Taxon
- tea plantse0
- Treatment
- suppression of CsCuAO1e0
- Gene
- CsCuAO1e0
- Outcome
- sensitivity to drought treatmente0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“the suppression of CsCuAO1 or CsAMADH1 in tea plants resulted in increased sensitivity to drought treatment”
Claim · model-written summary
Suppressing CsAMADH1 increased tea sensitivity to drought.
- Taxon
- tea plantse0
- Treatment
- suppression of CsCuAO1 or CsAMADH1e0
- Gene
- CsAMADH1e0
- Outcome
- sensitivity to drought treatmente0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“the suppression of CsCuAO1 or CsAMADH1 in tea plants resulted in increased sensitivity to drought treatment”
Claim · model-written summary
Co-overexpression increased GABA accumulation in Nicotiana benthamiana transient assays.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- co-overexpressinge0
- Outcome
- GABA accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“co-overexpressing plants increased GABA accumulation both in an Agrobacterium-mediated Nicotiana benthamiana transient assay and transgenic Arabidopsis plants”
Claim · model-written summary
Co-overexpression increased GABA accumulation in transgenic Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- co-overexpressinge0
- Outcome
- GABA accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“co-overexpressing plants increased GABA accumulation both in an Agrobacterium-mediated Nicotiana benthamiana transient assay and transgenic Arabidopsis plants”
Claim · model-written summary
CsGAT1 was identified as a GABA transporter gene.
Verbatim
e0“a GABA transporter gene, CsGAT1, was identified”
t0“tea plants”
Claim · model-written summary
CsGAT1 expression correlated strongly with GABA accumulation across tissues under drought stress.
- Taxon
- tea plantse1
- Organ / stage
- different tissuese0
- Gene
- CsGAT1t0
- Outcome
- GABA accumulation levelse0
- Qualifier
- under drought stresse0
Not reportedDirection
Verbatim
e0“whose expression was strongly correlated with GABA accumulation levels in different tissues under drought stress”
t0“CsGAT1”
e1“Camellia sinensis (Cs) CsCuAO1 associated with CsAMADH1 conferred drought tolerance, which modulated GABA levels in tea plants”
The same questions in other papers
application · pest · Camellia sinensis (black tea)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
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