Paper · 2023 · The Pakistan Journal of Agricultural Sciences

Traditional pathology and phylogenetic networking confirm the pathogen causing date palm wilt

Nabeeha Aslam Khan

10
claims
22
verbatim quotes
0
citations
2
research areas
✓
Open access

Abstract

Phoenix dactylifera L. (Date palm) is one of the leading perennial fruit crops regarding its nutritional profile, medicinal significance, excellent yields, long life, and high adaptability to diverse climatic conditions of semi-arid and arid regions globally. Eleven major palm-growing districts of Punjab, Sindh, and Khyber Pakhtunkhwa were surveyed for wilt disease assessment on date palms. The date palm plantation was highly affected with Fusarium wilt diseases with 55%, the incidence in Khairpur (Sindh province), followed by Bahawalpur 47.4% (Punjab Province) and 45% in Dera Ismail Khan (KPK province). Disease severity was recorded as 65% in Khairpur, 55% in Bahawalpur, 50% in Dera Ismail Khan (Sindh, Punjab, and KPK, respectively). Sixty-three Fusarium isolates were obtained from three hundred and eighty (380) diseased samples, and thirty isolates randomly selected out of 63 were studied for morpho-cultural and microscopic characterization. Morpho-cultural characters i.e colony pigmentation, growth pattern, conidia (macro and microconidia), shape, apical cell shape, and phalidies studied were coinciding with typical characters of Fusarium oxysporum. Twenty isolates of Fusarium population was subjected for the ITS region sequence data-based phylogenetic network. The Neighbor net algorithm computed an unrooted phylogenetic network displayed as four branched genetic-network that depicted the division of twenty fungal isolates into four groups. F. oxysporum (FMB-FO-PD-011) inoculated onto healthy symptomless detached date palm leaves, seedlings and suckers under controlled as well as field conditions showed typical wilt disease symptoms. Hence this study proved that F. oxysporum is the potential cause of date palm wilt in Pakistan.

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Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

Fusarium wilt incidence in date palm was 55% in Khairpur.

Taxon
date palmPhoenix dactyliferat0
Interactor
FusariumFusariume1pathogen
Outcome
incidencee1
Value
55%e1
Setting
Khairpure1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗
Fusarium
Fusarium · Interactor · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

t0

“confirm the pathogen causing date palm wilt”

✓ located verbatim in the source · title, characters 50–93 · show in text ↑
e1

“The date palm plantation was highly affected with Fusarium wilt diseases with 55%, the incidence in Khairpur (Sindh province)”

✓ located verbatim in the source · abstract, characters 394–519 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fusarium wilt incidence in date palm was 47.4% in Bahawalpur.

Taxon
date palmPhoenix dactyliferat0
Interactor
FusariumFusariume1pathogen
Outcome
incidencee1
Value
47.4%e2
Setting
Bahawalpure2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗
Fusarium
Fusarium · Interactor · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

t0

“confirm the pathogen causing date palm wilt”

✓ located verbatim in the source · title, characters 50–93 · show in text ↑
e1

“The date palm plantation was highly affected with Fusarium wilt diseases with 55%, the incidence in Khairpur (Sindh province)”

✓ located verbatim in the source · abstract, characters 394–519 · show in text ↑
e2

“followed by Bahawalpur 47.4% (Punjab Province)”

✓ located verbatim in the source · abstract, characters 521–567 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fusarium wilt incidence in date palm was 45% in Dera Ismail Khan.

Taxon
date palmPhoenix dactyliferat0
Interactor
FusariumFusariume1pathogen
Outcome
incidencee1
Value
45%e3
Setting
Dera Ismail Khane3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗
Fusarium
Fusarium · Interactor · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

t0

“confirm the pathogen causing date palm wilt”

✓ located verbatim in the source · title, characters 50–93 · show in text ↑
e1

“The date palm plantation was highly affected with Fusarium wilt diseases with 55%, the incidence in Khairpur (Sindh province)”

✓ located verbatim in the source · abstract, characters 394–519 · show in text ↑
e3

“45% in Dera Ismail Khan (KPK province)”

✓ located verbatim in the source · abstract, characters 572–610 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Disease severity of date palm wilt was 65% in Khairpur.

Taxon
date palmPhoenix dactyliferat0
Outcome
Disease severitye4
Value
65%e4
Setting
Khairpure4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“confirm the pathogen causing date palm wilt”

✓ located verbatim in the source · title, characters 50–93 · show in text ↑
e4

“Disease severity was recorded as 65% in Khairpur, 55% in Bahawalpur, 50% in Dera Ismail Khan (Sindh, Punjab, and KPK, respectively)”

✓ located verbatim in the source · abstract, characters 612–743 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Disease severity of date palm wilt was 55% in Bahawalpur.

Taxon
date palmPhoenix dactyliferat0
Outcome
Disease severitye4
Value
55%e4
Setting
Bahawalpure4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“confirm the pathogen causing date palm wilt”

✓ located verbatim in the source · title, characters 50–93 · show in text ↑
e4

“Disease severity was recorded as 65% in Khairpur, 55% in Bahawalpur, 50% in Dera Ismail Khan (Sindh, Punjab, and KPK, respectively)”

✓ located verbatim in the source · abstract, characters 612–743 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Disease severity of date palm wilt was 50% in Dera Ismail Khan.

Taxon
date palmPhoenix dactyliferat0
Outcome
Disease severitye4
Value
50%e4
Setting
Dera Ismail Khane4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“confirm the pathogen causing date palm wilt”

✓ located verbatim in the source · title, characters 50–93 · show in text ↑
e4

“Disease severity was recorded as 65% in Khairpur, 55% in Bahawalpur, 50% in Dera Ismail Khan (Sindh, Punjab, and KPK, respectively)”

✓ located verbatim in the source · abstract, characters 612–743 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Morpho-cultural characters of the isolates coincided with typical characters of Fusarium oxysporum.

Taxon
date palmPhoenix dactyliferat0
Interactor
Fusarium oxysporumFusarium oxysporume5pathogen
Outcome
Morpho-cultural characterse5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

t0

“confirm the pathogen causing date palm wilt”

✓ located verbatim in the source · title, characters 50–93 · show in text ↑
e5

“Morpho-cultural characters i.e colony pigmentation, growth pattern, conidia (macro and microconidia), shape, apical cell shape, and phalidies studied were coinciding with typical characters of Fusarium oxysporum”

✓ located verbatim in the source · abstract, characters 958–1169 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The phylogenetic network divided the twenty fungal isolates into four groups.

Taxon
date palmPhoenix dactyliferat0
Outcome
division of twenty fungal isolates into four groupse6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“confirm the pathogen causing date palm wilt”

✓ located verbatim in the source · title, characters 50–93 · show in text ↑
e6

“depicted the division of twenty fungal isolates into four groups”

✓ located verbatim in the source · abstract, characters 1402–1466 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

F. oxysporum inoculated onto healthy detached date palm leaves, seedlings and suckers produced typical wilt symptoms under controlled and field conditions.

Taxon
date palmPhoenix dactyliferat0
Organ / stage
leaves, seedlings and suckerse7
Treatment
inoculated onto healthy symptomless detached date palm leavese7
Interactor
F. oxysporum (FMB-FO-PD-011)Fusarium oxysporume7pathogen
Outcome
typical wilt disease symptomse7
Setting
controlled as well as field conditionse7

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

t0

“confirm the pathogen causing date palm wilt”

✓ located verbatim in the source · title, characters 50–93 · show in text ↑
e7

“F. oxysporum (FMB-FO-PD-011) inoculated onto healthy symptomless detached date palm leaves, seedlings and suckers under controlled as well as field conditions showed typical wilt disease symptoms”

✓ located verbatim in the source · abstract, characters 1468–1663 · show in text ↑
Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

F. oxysporum is the potential cause of date palm wilt in Pakistan.

Taxon
date palmPhoenix dactyliferat0
Interactor
F. oxysporumFusarium oxysporume2pathogen
Outcome
potential cause of date palm wilte2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

t0

“confirm the pathogen causing date palm wilt”

✓ located verbatim in the source · title, characters 50–93 · show in text ↑
e2

“Hence this study proved that F. oxysporum is the potential cause of date palm wilt in Pakistan”

✓ located verbatim in the source · abstract, characters 1665–1759 · show in text ↑

inoculation · Fusarium oxysporum · Phoenix dactylifera (date palm)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.