Abstract
Rice (Oryza sativa L.) production is threatened by global warming associated with extreme high temperatures, and rice heat sensitivity is differed when stress occurs between daytime and nighttime. However, the underlying molecular mechanism are largely unknown. We show here that two glycine-rich RNA binding proteins, OsGRP3 and OsGRP162, are required for thermotolerance in rice, especially at nighttime. The rhythmic expression of OsGRP3/OsGRP162 peaks at midnight, and at these coincident times, is increased by heat stress. This is largely dependent on the evening complex component OsELF3-2. We next found that the double mutant of OsGRP3/OsGRP162 is strikingly more sensitive to heat stress in terms of survival rate and seed setting rate when comparing to the wild-type plants. Interestingly, the defect in thermotolerance is more evident when heat stress occurred in nighttime than that in daytime. Upon heat stress, the double mutant of OsGRP3/OsGRP162 displays globally reduced expression of heat-stress responsive genes, and increases of mRNA alternative splicing dominated by exon-skipping. This study thus reveals the important role of OsGRP3/OsGRP162 in thermotolerance in rice, and unravels the mechanism on how OsGRP3/OsGRP162 regulate thermotolerance in a diurnal manner.
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Claims 8
Claim · model-written summary
OsGRP3 and OsGRP162 are required for heat tolerance, especially at night.
- Taxon
- rice
Oryza sativat0 - Gene
- OsGRP3 and OsGRP162e0
- Outcome
- thermotolerancee0
- Qualifier
- especially at nighttimee0
Not reportedMaterialOrgan / stageInteractorTreatmentComparatorSettingDirectionValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsGRP3 and OsGRP162, are required for thermotolerance in rice, especially at nighttime.”
t0“rice”
Claim · model-written summary
OsGRP3 and OsGRP162 expression peaks at midnight and rises under heat stress.
- Taxon
- riceOryza sativat0
- Gene
- OsGRP3/OsGRP162e0
- Outcome
- expression of OsGRP3/OsGRP162e0
- Direction
- increasede0
- Qualifier
- midnighte0
Not reportedMaterialOrgan / stageInteractorTreatmentComparatorSettingValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rhythmic expression of OsGRP3/OsGRP162 peaks at midnight, and at these coincident times, is increased by heat stress.”
t0“rice”
Claim · model-written summary
The heat-induced rhythmic expression largely depends on OsELF3-2.
- Taxon
- riceOryza sativat0
- Gene
- OsELF3-2e0
- Outcome
- largely dependente0
Not reportedMaterialOrgan / stageInteractorTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This is largely dependent on the evening complex component OsELF3-2.”
t0“rice”
Claim · model-written summary
The double mutant had lower heat-stress survival than wild type.
- Taxon
- riceOryza sativat0
- Material
- double mutant of OsGRP3/OsGRP162e0
- Treatment
- heat stresse0
- Outcome
- survival ratee0
- Direction
- more sensitivee0
- Comparator
- wild-type plantse0
Not reportedGeneOrgan / stageInteractorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the double mutant of OsGRP3/OsGRP162 is strikingly more sensitive to heat stress in terms of survival rate and seed setting rate when comparing to the wild-type plants.”
t0“rice”
Claim · model-written summary
The double mutant had lower seed setting under heat than wild type.
- Taxon
- riceOryza sativat0
- Material
- double mutant of OsGRP3/OsGRP162e0
- Treatment
- heat stresse0
- Outcome
- seed setting ratee0
- Direction
- more sensitivee0
- Comparator
- wild-type plantse0
Not reportedGeneOrgan / stageInteractorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the double mutant of OsGRP3/OsGRP162 is strikingly more sensitive to heat stress in terms of survival rate and seed setting rate when comparing to the wild-type plants.”
t0“rice”
Claim · model-written summary
The thermotolerance defect was greater under night than daytime heat.
- Taxon
- riceOryza sativat0
- Treatment
- heat stress occurred in nighttimee0
- Outcome
- defect in thermotolerancee0
- Direction
- more evidente0
- Comparator
- daytimee0
Not reportedMaterialGeneOrgan / stageInteractorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the defect in thermotolerance is more evident when heat stress occurred in nighttime than that in daytime.”
t0“rice”
Claim · model-written summary
Heat-stressed double mutants had globally reduced heat-response gene expression.
- Taxon
- riceOryza sativat0
- Material
- double mutant of OsGRP3/OsGRP162e0
- Treatment
- heat stresse0
- Outcome
- expression of heat-stress responsive genese0
- Direction
- reducede0
Not reportedGeneOrgan / stageInteractorComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon heat stress, the double mutant of OsGRP3/OsGRP162 displays globally reduced expression of heat-stress responsive genes”
t0“rice”
Claim · model-written summary
Heat-stressed double mutants had increased mRNA alternative splicing dominated by exon skipping.
- Taxon
- riceOryza sativat0
- Outcome
- mRNA alternative splicinge0
- Direction
- increasese0
- Qualifier
- exon-skippinge0
Not reportedMaterialGeneOrgan / stageInteractorTreatmentComparatorSettingValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increases of mRNA alternative splicing dominated by exon-skipping.”
t0“rice”
The same questions in other papers
heat · tolerance: abiotic · Oryza sativa (rice)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.