Abstract
Enzymatic browning causes a decline in consumer’s acceptance of fresh-cut products. Previous study showed reactive oxygen species (ROS) is associated with enzymatic browning, and prohibitin (PHB) is involved in ROS formation. However, whether PHB influences the enzymatic browning is still unknown. In this paper, changes of StPHB3 transcript levels after cutting were investigated, then stphb3 mutants were constructed through CRISPR/Cas9 and the browning difference between stphb3 mutants and wild type was compared, finally the mechanism of PHB influencing the browning was explored. The results showed that the transcript levels of StPHB3 were enhanced in potato (cultivar Desiree ) tubers after fresh cutting. Mutation of StPHB3 caused lower browning, less production of soluble quinones, decreased malondialdehyde (MDA) content, increased 1,1-Diphenyl-2-picrylhydrazyl (DPPH) inhibition, and increased total phenol contents. Additionally, polyphenol oxidase (PPO) activity and StPOT32 gene transcript level were also reduced after disturbing StPHB3 gene expression. StPHB3 was found to be located within the chloroplast same as the predominant location and activation site of PPO protein. Yeast two-hybrid (Y2H) and Co-Immunoprecipitation (Co-IP) assays showed StPHB3 interacted with PPO. And mature PPO contents were decreased in stphb3 . These results suggest that suppression of StPHB3 can alleviate the browning of fresh-cut potatoes by enhancing the antioxidant activity and inhibiting PPO activation.
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Claims 9
Claim · model-written summary
StPHB3 transcript levels rose in potato tubers after cutting.
- Taxon
- potato
Solanum tuberosume0 - Material
- Desireee0
- Organ / stage
- tuberse0
- Gene
- StPHB3e0
- Outcome
- transcript levels of StPHB3e0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcript levels of StPHB3 were enhanced in potato (cultivar Desiree ) tubers after fresh cutting”
Claim · model-written summary
StPHB3 mutation reduced fresh-cut potato browning.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- Mutation of StPHB3e0
- Gene
- StPHB3e0
- Outcome
- browninge0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of StPHB3 caused lower browning”
t0“potatoes”
Claim · model-written summary
StPHB3 mutation reduced soluble quinone production.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- Mutation of StPHB3e0
- Outcome
- production of soluble quinonese0
- Direction
- lesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of StPHB3 caused lower browning, less production of soluble quinones”
t0“potatoes”
Claim · model-written summary
StPHB3 mutation decreased malondialdehyde content.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- Mutation of StPHB3e0
- Outcome
- malondialdehyde (MDA) contente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of StPHB3 caused lower browning, less production of soluble quinones, decreased malondialdehyde (MDA) content”
t0“potatoes”
Claim · model-written summary
Disrupting StPHB3 reduced polyphenol oxidase activity.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- disturbing StPHB3 gene expressione0
- Gene
- StPHB3e0
- Outcome
- polyphenol oxidase (PPO) activitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“polyphenol oxidase (PPO) activity and StPOT32 gene transcript level were also reduced after disturbing StPHB3 gene expression”
t0“potatoes”
Claim · model-written summary
Disrupting StPHB3 reduced StPOT32 transcript level.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- disturbing StPHB3 gene expressione0
- Gene
- StPOT32e0
- Outcome
- StPOT32 gene transcript levele0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“polyphenol oxidase (PPO) activity and StPOT32 gene transcript level were also reduced after disturbing StPHB3 gene expression”
t0“potatoes”
Claim · model-written summary
StPHB3 was located in the chloroplast.
- Taxon
- potatoesSolanum tuberosumt0
- Gene
- StPHB3e0
- Outcome
- located within the chloroplaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StPHB3 was found to be located within the chloroplast”
t0“potatoes”
Claim · model-written summary
StPHB3 interacted with PPO in protein assays.
- Taxon
- potatoesSolanum tuberosumt0
- Gene
- StPHB3e0
- Outcome
- StPHB3 interacted with PPOe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StPHB3 interacted with PPO”
t0“potatoes”
Claim · model-written summary
Mature PPO content decreased in stphb3 mutants.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- stphb3e0
- Gene
- PPOe0
- Outcome
- mature PPO contentse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature PPO contents were decreased in stphb3”
t0“potatoes”
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