Abstract
Abstract Here, a sucrose non‐fermenting‐1‐related protein kinase alpha subunit (TaSnRK1α‐1A) is identified as associated with cold stress through integration of genome‐wide association study, bulked segregant RNA sequencing, and virus‐induced gene silencing. It is confirmed that TaSnRK1α positively regulates cold tolerance by transgenes and ethyl methanesulfonate (EMS) mutants. A plastid‐lipid‐associated protein 6, chloroplastic‐like (TaPAP6L‐2B) strongly interacting with TaSnRK1α‐1A is screened. Molecular chaperone DJ‐1 family protein (TaDJ‐1‐7B) possibly bridged the interaction of TaSnRK1α‐1A and TaPAP6L‐2B. It is further revealed that TaSnRK1α‐1A phosphorylated TaPAP6L‐2B. Subsequently, a superior haplotype TaPAP6L‐2B 30S /38S is identified and confirmed that both R30S and G38S are important phosphorylation sites that influence TaPAP6L‐2B in cold tolerance. Overexpression (OE) and EMS‐mutant lines verified TaPAP6L positively modulating cold tolerance. Furthermore, transcriptome sequencing revealed that TaPAP6L‐2B‐OE lines significantly increased jasmonic acid (JA) content, possibly by improving precursor α‐linolenic acid contributing to JA synthesis and by repressing JAR1 degrading JA. Exogenous JA significantly improved the cold tolerance of wheat plants. In summary, TaSnRK1α profoundly regulated cold stress, possibly through phosphorylating TaPAP6L to increase endogenous JA content of wheat plants.
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Claims 12
Claim · model-written summary
TaSnRK1α‐1A was associated with cold stress.
- Taxon
- Wheat
Triticum aestivumt0 - Gene
- TaSnRK1α‐1Ae0
- Outcome
- cold stresse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“TaSnRK1α‐1A) is identified as associated with cold stress”
Claim · model-written summary
TaSnRK1α positively regulates wheat cold tolerance.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaSnRK1αe0
- Outcome
- cold tolerancee0
- Direction
- positively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“TaSnRK1α positively regulates cold tolerance by transgenes and ethyl methanesulfonate (EMS) mutants”
Claim · model-written summary
TaPAP6L‐2B strongly interacts with TaSnRK1α‐1A.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaPAP6L‐2Be0
- Outcome
- strongly interacting with TaSnRK1α‐1Ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“TaPAP6L‐2B) strongly interacting with TaSnRK1α‐1A is screened”
Claim · model-written summary
TaDJ‐1‐7B may bridge the TaSnRK1α‐1A and TaPAP6L‐2B interaction.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaDJ‐1‐7Be0
- Outcome
- interaction of TaSnRK1α‐1A and TaPAP6L‐2Be0
- Qualifier
- possiblye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“TaDJ‐1‐7B) possibly bridged the interaction of TaSnRK1α‐1A and TaPAP6L‐2B”
Claim · model-written summary
TaSnRK1α‐1A phosphorylated TaPAP6L‐2B.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaSnRK1α‐1Ae0
- Outcome
- phosphorylated TaPAP6L‐2Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“TaSnRK1α‐1A phosphorylated TaPAP6L‐2B”
Claim · model-written summary
A superior TaPAP6L‐2B 30S /38S haplotype was identified.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaPAP6L‐2B 30S /38Se0
- Outcome
- a superior haplotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“a superior haplotype TaPAP6L‐2B 30S /38S is identified”
Claim · model-written summary
R30S and G38S phosphorylation sites influence TaPAP6L‐2B in cold tolerance.
- Taxon
- WheatTriticum aestivumt0
- Gene
- R30S and G38Se0
- Outcome
- cold tolerancee0
- Qualifier
- important phosphorylation sitese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“both R30S and G38S are important phosphorylation sites that influence TaPAP6L‐2B in cold tolerance”
Claim · model-written summary
TaPAP6L positively modulates cold tolerance.
- Taxon
- WheatTriticum aestivumt0
- Gene
- TaPAP6Le0
- Outcome
- cold tolerancee0
- Direction
- positively modulatinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“TaPAP6L positively modulating cold tolerance”
Claim · model-written summary
TaPAP6L‐2B overexpression lines had significantly increased jasmonic acid content.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- TaPAP6L‐2B‐OEe0
- Outcome
- jasmonic acid (JA) contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“TaPAP6L‐2B‐OE lines significantly increased jasmonic acid (JA) content”
Claim · model-written summary
Increased jasmonic acid may involve improved α‐linolenic acid precursor availability.
- Taxon
- WheatTriticum aestivumt0
- Compound
- α‐linolenic acide0
- Outcome
- JA synthesise0
- Qualifier
- possiblye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“possibly by improving precursor α‐linolenic acid contributing to JA synthesis”
Claim · model-written summary
Increased jasmonic acid may involve repression of JAR1 that degrades JA.
- Taxon
- WheatTriticum aestivumt0
- Gene
- JAR1e0
- Outcome
- JAe0
- Qualifier
- repressing JAR1 degrading JAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“by repressing JAR1 degrading JA”
Claim · model-written summary
Exogenous JA significantly improved wheat cold tolerance.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Exogenous JAe0
- Outcome
- cold tolerancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous JA significantly improved the cold tolerance of wheat plants”
The same questions in other papers
jasmonic acid · tolerance: abiotic · Triticum aestivum (wheat)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.