Paper · 2023 · Menoufia Journal of Plant Protection

STUDIES ON DOWNY MILDEW DISEASE OF CUCURBITS AND IT'S CONTROL METHODS.

M. M Ammar; S. N Draz; H. M Awad; et al.

16
claims
30
verbatim quotes
0
citations
3
research areas
✓
Open access

Abstract

Downy mildew of cucurbits is very important disease worldwide. This investigation was carried out in order to achieve safe control method(s) to such disease. The obtained results courld be summarized as following: Pseudoperonospora cubensis is the causal organism of downy mildew disease of either cucumber or squash and it was first recorded in Egypt on watermelon at 1935. Spraying cucumber plants with the tested plant extracts reduced both percentage of infection (P.I.) and the disease severity (D.S.) in Comparison with the untreated control treatment, significantly. Yield production was also increased significantly. Thuja plant water extract showed the best efficiency in reducing the disease incidence, followed by clove one while black cumine plant extract showed the least efficiency. The application of the plant essential oils greatly reduced downy mildew disease of cucumber and squash cultivar, and increased yield production in comparison with the untreated control plants. Clove essential oil followed by mint one gave the best results. Application of the tested growth regulators to cucumber (Madaen cv.) and Squash (Aziad cv.) plants significantly reduced the disease incidence and improved yield production, in comparison with control, both at 2018-2019 and 2019-2020 growing seasons. In this request, Cytokinin and Gibberellin showed the best efficiency, while Auxin was the least effective one. Foliage fertilization with NPK fertilizers greatly affected cucumber and squash cultivar downy mildew diseases incidence and increased yield production compared to control, significantly. However, it was noticed that increasing nitrogen doses led to the increment of both (P.I.) and (D.S.). The best yield production was obtained when the highest level of potassium was applied. Application of Cabriodio 11.2% fungicide suppressed the disease incidence, completely. In the meantime, high increment of yield production was obtained, compared to the other treatments. Growth characteristics of squash plants i.e., plant height, leaf area, root length, shoot length, plant fresh weight and plant dry weight were significantly increased in response to the application of any tested control methods compared to the untreated control plants. 10. Total water content (TWC), relative water content (RWC) and transpiration rate of squash plants were remarkably increased in response to the application of either Gibberellin, black cumin oil, clove oil (10%), mint oil (5%), Thuja extract and/or the fungicide in comparison with the untreated control. 11. Application of the disease control methods to squash plants increased chlorophyll, total sugars content and proline concentration compared to the untreated control plants. 12. Chemical analysis of N. sativa essential oil indicate that this oil mainly contained vanillic acid (89.94 mg/ml) and gallic acid (29.59 mg/ml). 13. Peppermint oil contained gallic acid (18.6 mg/ml) and caffeic acid (4.8 mg/ml). 14. Clove essential oil has high amount of eugenol (72.72 mg/ml), eugenol acetate (2.1 mg/ml), guaicol (4.16 mg/ml) and methyl quaiacol (9.79 mg/ml). 15. Thuja plant water extract contained tannic acid (38.16 mg/100 g sample) and catechin (33.54 mg/100 gm). 16. The most frequently present compounds in clove plant extract was eugenol (79.72 mg/100 g sample) and limonin (3.52 mg/100 g sample).

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Claims 16

Observationobservedoriginal resultmeasured

Claim · model-written summary

Pseudoperonospora cubensis was identified as the cause of cucumber downy mildew.

Taxon
cucumberCucumis sativuse0
Interactor
Pseudoperonospora cubensisPseudoperonospora cubensise0pathogen
Outcome
causal organism of downy mildew diseasee0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Pseudoperonospora cubensis
Pseudoperonospora cubensis · InteractorChristian Hummert ( Ixitixel ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Pseudoperonospora cubensis is the causal organism of downy mildew disease of either cucumber or squash”

✓ located verbatim in the source · abstract, characters 214–316 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Pseudoperonospora cubensis was identified as the cause of squash downy mildew.

Taxon
squashe0
Interactor
Pseudoperonospora cubensisPseudoperonospora cubensise0pathogen
Outcome
causal organism of downy mildew diseasee0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pseudoperonospora cubensis
Pseudoperonospora cubensis · InteractorChristian Hummert ( Ixitixel ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Pseudoperonospora cubensis is the causal organism of downy mildew disease of either cucumber or squash”

✓ located verbatim in the source · abstract, characters 214–316 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Plant extract sprays significantly reduced cucumber downy mildew infection percentage and severity.

Taxon
cucumberCucumis sativuse0
Treatment
tested plant extractse0
Interactor
Pseudoperonospora cubensisPseudoperonospora cubensise1pathogent0
Outcome
percentage of infection (P.I.) and the disease severity (D.S.)e0
Direction
reducede0
Comparator
untreated control treatmente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Pseudoperonospora cubensis
Pseudoperonospora cubensis · InteractorChristian Hummert ( Ixitixel ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Spraying cucumber plants with the tested plant extracts reduced both percentage of infection (P.I.) and the disease severity (D.S.) in Comparison with the untreated control treatment, significantly”

✓ located verbatim in the source · abstract, characters 375–572 · show in text ↑
t0

“DOWNY MILDEW”

✓ located verbatim in the source · title, characters 11–23 · show in text ↑
e1

“Pseudoperonospora cubensis is the causal organism of downy mildew disease of either cucumber or squash”

✓ located verbatim in the source · abstract, characters 214–316 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Plant extract sprays significantly increased cucumber yield.

Taxon
cucumberCucumis sativuse1
Treatment
tested plant extractse1
Outcome
Yield productione0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Yield production was also increased significantly”

✓ located verbatim in the source · abstract, characters 574–623 · show in text ↑
t0

“CUCURBITS”

✓ located verbatim in the source · title, characters 35–44 · show in text ↑
e1

“Spraying cucumber plants with the tested plant extracts reduced both percentage of infection (P.I.) and the disease severity (D.S.) in Comparison with the untreated control treatment, significantly”

✓ located verbatim in the source · abstract, characters 375–572 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Thuja extract was most efficient against downy mildew, followed by clove and black cumine least.

Taxon
cucumberCucumis sativuse1
Treatment
Thuja plant water extracte0
Outcome
disease incidencee0
Direction
best efficiency in reducinge0
Comparator
clove onee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Thuja plant water extract showed the best efficiency in reducing the disease incidence, followed by clove one while black cumine plant extract showed the least efficiency”

✓ located verbatim in the source · abstract, characters 625–795 · show in text ↑
t0

“CUCURBITS”

✓ located verbatim in the source · title, characters 35–44 · show in text ↑
e1

“Spraying cucumber plants with the tested plant extracts reduced both percentage of infection (P.I.) and the disease severity (D.S.) in Comparison with the untreated control treatment, significantly”

✓ located verbatim in the source · abstract, characters 375–572 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Essential oils reduced cucumber downy mildew and increased yield.

Taxon
cucumberCucumis sativuse0
Treatment
plant essential oilse0
Outcome
downy mildew diseasee0
Direction
reducede0
Comparator
untreated control plantse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The application of the plant essential oils greatly reduced downy mildew disease of cucumber and squash cultivar, and increased yield production in comparison with the untreated control plants”

✓ located verbatim in the source · abstract, characters 797–989 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Essential oils reduced squash downy mildew.

Taxon
squashe0
Treatment
plant essential oilse0
Outcome
downy mildew diseasee0
Direction
reducede0
Comparator
untreated control plantse0

Verbatim

e0

“The application of the plant essential oils greatly reduced downy mildew disease of cucumber and squash cultivar, and increased yield production in comparison with the untreated control plants”

✓ located verbatim in the source · abstract, characters 797–989 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Essential oils increased cucumber and squash yield.

Taxon
cucumber and squashe1
Treatment
plant essential oilse1
Outcome
yield productione0
Direction
increasede0
Comparator
untreated control plantse0

Verbatim

e0

“increased yield production in comparison with the untreated control plants”

✓ located verbatim in the source · abstract, characters 915–989 · show in text ↑
t0

“CUCURBITS”

✓ located verbatim in the source · title, characters 35–44 · show in text ↑
e1

“The application of the plant essential oils greatly reduced downy mildew disease of cucumber and squash cultivar, and increased yield production in comparison with the untreated control plants”

✓ located verbatim in the source · abstract, characters 797–989 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Growth regulators reduced cucumber disease incidence and improved yield.

Taxon
cucumberCucumis sativuse0
Material
Madaen cv.e0
Treatment
tested growth regulatorse0
Outcome
disease incidencee0
Direction
reducede0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Application of the tested growth regulators to cucumber (Madaen cv.) and Squash (Aziad cv.) plants significantly reduced the disease incidence and improved yield production, in comparison with control”

✓ located verbatim in the source · abstract, characters 1055–1255 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Growth regulators reduced squash disease incidence and improved yield.

Taxon
Squashe0
Material
Aziad cv.e0
Treatment
tested growth regulatorse0
Outcome
yield productione0
Direction
improvede0
Comparator
controle0

Verbatim

e0

“Application of the tested growth regulators to cucumber (Madaen cv.) and Squash (Aziad cv.) plants significantly reduced the disease incidence and improved yield production, in comparison with control”

✓ located verbatim in the source · abstract, characters 1055–1255 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Nitrogen dose increases raised infection percentage and disease severity.

Taxon
cucumber and squashe1
Treatment
nitrogen dosese0
Outcome
(P.I.) and (D.S.)e0
Direction
incremente0

Verbatim

e0

“increasing nitrogen doses led to the increment of both (P.I.) and (D.S.)”

✓ located verbatim in the source · abstract, characters 1635–1707 · show in text ↑
t0

“CUCURBITS”

✓ located verbatim in the source · title, characters 35–44 · show in text ↑
e1

“The application of the plant essential oils greatly reduced downy mildew disease of cucumber and squash cultivar, and increased yield production in comparison with the untreated control plants”

✓ located verbatim in the source · abstract, characters 797–989 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Highest potassium level gave the best yield.

Taxon
cucumber and squashe1
Treatment
highest level of potassiume0
Outcome
yield productione0
Direction
beste0

Not reportedComparator

Verbatim

e0

“The best yield production was obtained when the highest level of potassium was applied”

✓ located verbatim in the source · abstract, characters 1709–1795 · show in text ↑
t0

“CUCURBITS”

✓ located verbatim in the source · title, characters 35–44 · show in text ↑
e1

“The application of the plant essential oils greatly reduced downy mildew disease of cucumber and squash cultivar, and increased yield production in comparison with the untreated control plants”

✓ located verbatim in the source · abstract, characters 797–989 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cabriodio fungicide completely suppressed downy mildew incidence.

Taxon
cucumber and squashe1
Treatment
Cabriodio 11.2% fungicidee0
Outcome
disease incidencee0
Direction
suppressede0

Not reportedComparator

Verbatim

e0

“Application of Cabriodio 11.2% fungicide suppressed the disease incidence, completely”

✓ located verbatim in the source · abstract, characters 1797–1882 · show in text ↑
t0

“CUCURBITS”

✓ located verbatim in the source · title, characters 35–44 · show in text ↑
e1

“The application of the plant essential oils greatly reduced downy mildew disease of cucumber and squash cultivar, and increased yield production in comparison with the untreated control plants”

✓ located verbatim in the source · abstract, characters 797–989 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Disease control methods significantly increased squash growth characteristics.

Taxon
squashe0
Treatment
any tested control methodse0
Outcome
plant height, leaf area, root lengthe0
Direction
increasede0
Comparator
untreated control plantse0

Verbatim

e0

“Growth characteristics of squash plants i.e., plant height, leaf area, root length, shoot length, plant fresh weight and plant dry weight were significantly increased in response to the application of any tested control methods compared to the untreated control plants”

✓ located verbatim in the source · abstract, characters 1984–2252 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Control treatments raised squash water content and transpiration.

Taxon
squashe0
Treatment
Gibberellin, black cumin oil, clove oil (10%), mint oil (5%), Thuja extract and/or the fungicidee0
Outcome
Total water content (TWC), relative water content (RWC) and transpiration ratee0
Direction
increasede0
Comparator
untreated controle0

Verbatim

e0

“Total water content (TWC), relative water content (RWC) and transpiration rate of squash plants were remarkably increased in response to the application of either Gibberellin, black cumin oil, clove oil (10%), mint oil (5%), Thuja extract and/or the fungicide in comparison with the untreated control”

✓ located verbatim in the source · abstract, characters 2258–2558 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Disease control methods increased squash chlorophyll, sugar and proline.

Taxon
squashe0
Treatment
disease control methodse0
Outcome
chlorophyll, total sugars content and proline concentratione0
Direction
increasede0
Comparator
untreated control plantse0

Verbatim

e0

“Application of the disease control methods to squash plants increased chlorophyll, total sugars content and proline concentration compared to the untreated control plants”

✓ located verbatim in the source · abstract, characters 2564–2734 · show in text ↑

Thuja · soil: physics · Cucurbita (pumpkins)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Nigella sativa · soil: physics · Cucurbita (pumpkins)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Syzygium aromaticum · soil: physics · Cucurbita (pumpkins)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

botanical · soil: physics · Cucurbita (pumpkins)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

botanical · disease · Cucurbita (pumpkins)Positive and negative results

6 papers: 4 positive, 2 negative or no effect

3 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

2 papers more in this group

See all 6 papers →

potassium · yield · Cucurbita (pumpkins)Positive and negative results

8 papers: 7 positive, 1 negative or no effect

6 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

4 papers more in this group

See all 8 papers →

botanical · yield · Cucurbita (pumpkins)Positive and negative results

9 papers: 8 positive, 1 negative or no effect

7 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

fungicide · disease · Cucurbita (pumpkins)Positive and negative results

11 papers: 10 positive, 1 negative or no effect

9 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

7 papers more in this group

See all 11 papers →

8 of 17 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.