Abstract
The coastal area of Bangladesh is affected by soil salinity which threatens rice production. Saline soils are mainly caused by excessive Na+ which interferes with the uptake of the essential nutrient K+. Certain K+ transporters may cause leakage of Na+ ions and the OsAKT1 transporter was earlier shown to be downregulated under salt stress in a tolerant rice landrace called Horkuch. We therefore targeted this gene for Crispr-mediated downregulation by Agrobacterium-mediated in planta transformation in salt sensitive IR29 rice. The gene was shown to be significantly downregulated in at least 3 independent transformants. One of the gene edited IR29 transformants showed significantly high survival, low loss in chlorophyll content, low electrolyte leakage as well as low H2O2 after 15-days of salt stress at 120mM NaCl at the T3 generation. Therefore the potassium transporter OsAKT1 gene is a good target for downregulation to produce salt tolerant rice. Plant Tissue Cult. & Biotech. 33(1): 71-84, 2023 (June)
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Claims 6
Claim · model-written summary
OsAKT1 was significantly downregulated in at least three independent rice transformants.
- Taxon
- Rice
Oryza sativat0 - Material
- IR29t0
- Gene
- OsAKT1e1
- Outcome
- The genee0
- Direction
- downregulatede0
- Value
- at least 3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gene was shown to be significantly downregulated in at least 3 independent transformants.”
t0“Rice IR29”
e1“the OsAKT1 transporter was earlier shown to be downregulated under salt stress”
Claim · model-written summary
One edited IR29 transformant had significantly high survival after salt stress.
- Taxon
- RiceOryza sativat0
- Material
- IR29e0
- Treatment
- 15-days of salt stress at 120mM NaCle0
- Gene
- OsAKT1e1
- Outcome
- survivale0
- Direction
- highe0
- Qualifier
- at the T3 generatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One of the gene edited IR29 transformants showed significantly high survival, low loss in chlorophyll content, low electrolyte leakage as well as low H2O2 after 15-days of salt stress at 120mM NaCl at the T3 generation.”
t0“Rice IR29”
e1“the OsAKT1 transporter was earlier shown to be downregulated under salt stress”
Claim · model-written summary
The edited IR29 transformant had low loss of chlorophyll after salt stress.
- Taxon
- RiceOryza sativat0
- Treatment
- 15-days of salt stress at 120mM NaCle0
- Outcome
- loss in chlorophyll contente0
- Direction
- lowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One of the gene edited IR29 transformants showed significantly high survival, low loss in chlorophyll content, low electrolyte leakage as well as low H2O2 after 15-days of salt stress at 120mM NaCl at the T3 generation.”
t0“Rice IR29”
Claim · model-written summary
The edited IR29 transformant had low electrolyte leakage after salt stress.
- Taxon
- RiceOryza sativat0
- Treatment
- 15-days of salt stress at 120mM NaCle0
- Outcome
- electrolyte leakagee0
- Direction
- lowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One of the gene edited IR29 transformants showed significantly high survival, low loss in chlorophyll content, low electrolyte leakage as well as low H2O2 after 15-days of salt stress at 120mM NaCl at the T3 generation.”
t0“Rice IR29”
Claim · model-written summary
The edited IR29 transformant had low H2O2 after salt stress.
- Taxon
- RiceOryza sativat0
- Treatment
- 15-days of salt stress at 120mM NaCle0
- Outcome
- H2O2e0
- Direction
- lowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One of the gene edited IR29 transformants showed significantly high survival, low loss in chlorophyll content, low electrolyte leakage as well as low H2O2 after 15-days of salt stress at 120mM NaCl at the T3 generation.”
t0“Rice IR29”
Claim · model-written summary
The authors propose downregulating OsAKT1 to produce salt tolerant rice.
- Taxon
- riceOryza sativae0
- Gene
- OsAKT1e0
- Outcome
- downregulation to produce salt tolerant ricee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Therefore the potassium transporter OsAKT1 gene is a good target for downregulation to produce salt tolerant rice.”
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