Abstract
Recently, CRISPR-Cas9-based genome editing has been widely used for plant breeding. In our previous report, a tomato gene encoding hybrid proline-rich protein 1 (HyPRP1), a negative regulator of salt stress responses, has been edited using a CRISPR-Cas9 multiplexing approach that resulted in precise eliminations of its functional domains, proline-rich domain (PRD) and eight cysteine-motif (8CM). We subsequently demonstrated that eliminating the PRD domain of HyPRP1 in tomatoes conferred the highest level of salinity tolerance. In this study, we characterized the edited lines under several abiotic and biotic stresses to examine the possibility of multiple stress tolerance. Our data reveal that the 8CM removal variants of HK and the KO alleles of both HK and 15T01 cultivars exhibited moderate heat stress tolerance. Similarly, plants carrying either the domains of the PRD removal variant (PR1v1) or 8CM removal variants (PR2v2 and PR2v3) showed better germination under osmosis stress (up to 200 mM mannitol) compared to the WT control. Moreover, the PR1v1 line continuously grew after 5 days of water cutoff. When the edited lines were challenged with pathogenic bacteria of Pseudomonas syringae pv. tomato ( Pto ) DC3000, the growth of the bacterium was significantly reduced by 2.0- to 2.5-fold compared to that in WT plants. However, the edited alleles enhanced susceptibility against Fusarium oxysporum f. sp. lycopersici , which causes fusarium wilt. CRISPR-Cas9-based precise domain editing of the SlHyPRP1 gene generated multi-stress-tolerant alleles that could be used as genetic materials for tomato breeding.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Tomato lines with 8CM removal variants of HK and KO alleles of both HK and 15T01 cultivars exhibited moderate heat stress tolerance.
- Taxon
- tomato
Solanum lycopersicumt0 - Material
- 8CM removal variants of HK and the KO alleles of both HK and 15T01 cultivarse0
- Treatment
- heat stresse0
- Outcome
- heat stress tolerancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 8CM removal variants of HK and the KO alleles of both HK and 15T01 cultivars exhibited moderate heat stress tolerance”
t0“multi-stress tolerance in tomato”
Claim · model-written summary
Tomato plants carrying the PRD removal variant PR1v1 or the 8CM removal variants PR2v2 and PR2v3 showed better germination under osmosis stress (up to 200 mM mannitol) than the WT control.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- PRD removal variant (PR1v1) or 8CM removal variants (PR2v2 and PR2v3)e0
- Treatment
- osmosis stress (up to 200 mM mannitol)e0
- Outcome
- germinatione0
- Direction
- bettere0
- Comparator
- WT controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants carrying either the domains of the PRD removal variant (PR1v1) or 8CM removal variants (PR2v2 and PR2v3) showed better germination under osmosis stress (up to 200 mM mannitol) compared to the WT control”
t0“multi-stress tolerance in tomato”
Claim · model-written summary
The PR1v1 tomato line continued to grow after 5 days of water cutoff.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- PR1v1 linee0
- Outcome
- continuously grewe0
- Qualifier
- 5 days of water cutoffe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the PR1v1 line continuously grew after 5 days of water cutoff”
t0“multi-stress tolerance in tomato”
Claim · model-written summary
When challenged with Pseudomonas syringae pv. tomato DC3000, bacterial growth was significantly reduced 2.0- to 2.5-fold in the edited tomato lines compared with WT plants.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- edited linese0
- Interactor
- Pseudomonas syringae pv. tomato
Pseudomonas syringae pv. tomatoe0pathogen - Outcome
- growth of the bacteriume0
- Direction
- reducede0
- Value
- 2.0- to 2.5-folde0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the edited lines were challenged with pathogenic bacteria of Pseudomonas syringae pv. tomato ( Pto ) DC3000, the growth of the bacterium was significantly reduced by 2.0- to 2.5-fold compared to that in WT plants”
t0“multi-stress tolerance in tomato”
Claim · model-written summary
The edited tomato alleles enhanced susceptibility to Fusarium oxysporum f. sp. lycopersici.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- edited allelese0
- Interactor
- Fusarium oxysporum f. sp. lycopersici
Fusarium oxysporum f. sp. lycopersicie0pathogen - Outcome
- susceptibilitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the edited alleles enhanced susceptibility against Fusarium oxysporum f. sp. lycopersici”
t0“multi-stress tolerance in tomato”
Claim · model-written summary
Precise domain editing of the SlHyPRP1 gene generated multi-stress-tolerant alleles that could be used as genetic material for tomato breeding.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- SlHyPRP1e0
- Outcome
- multi-stress-tolerant allelese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRISPR-Cas9-based precise domain editing of the SlHyPRP1 gene generated multi-stress-tolerant alleles that could be used as genetic materials for tomato breeding”
The same questions in other papers
loss of function · Fusarium oxysporum f. sp. lycopersici · Solanum lycopersicum (tomato)Results agree
1 paper in the same direction as this paper
- negative
heat · tolerance: abiotic · Solanum lycopersicum (tomato)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.