Abstract
Nanoparticles have recently been employed as a new strategy to act as bactericides in agricultural applications. However, the effects and mechanisms of foliar deposition of nanoparticles on bacterial pathogens, plant physiology and particularly plant immunity have not been sufficiently understood. Here, we investigated the effects and mechanisms of ZnO NPs in controlling of tobacco wildfire caused by Pseudomonas syringae pv. tabaci, through the comprehensive analysis of biological changes of both bacteria and plants. The global gene expression changes of Pseudomonas syringae pv. tabaci supported that the functions of "protein secretion", "membrane part", "signal transducer activity", "locomotion", "chemotaxis" and "taxis" in bacteria, as well as the metabolic pathways of "bacterial chemotaxis", "two-component system", "biofilm formation", "ABC transporters" and "valine, leucine and isoleucine degradation" were significantly down-regulated by ZnO NPs. Correspondingly, we reconfirmed that the cell envelope structure, biofilm and motility of Pseudomonas syringae pv. tabaci were directly disrupted or suppressed by ZnO NPs. Different from completely killing Pseudomonas syringae pv. tabaci, ZnO NPs (0.5 mg/mL) potentially improved plant growth and immunity through enzymatic activity and global molecular response analysis. Furthermore, the changes of gene expression in ABA signaling pathway, ABA concentration and stomatal aperture all supported that ZnO NPs can specifically stimulate stomatal immunity, which is important to defend bacterial infection. Taken together, we proposed that both the inhibition or damage of motility, biofilm, metabolisms, virulence and cell envelope on P. syringae pv. tabaci, and the activation of the stomatal immunity formed two-layered antibacterial mechanisms of ZnO NPs on phytopathogenic bacteria.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
ZnO NPs significantly down-regulated global gene expression functions of Pseudomonas syringae pv. tabaci, including protein secretion, locomotion and chemotaxis.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- ZnO NPse1
- Interactor
- Pseudomonas syringae pv. tabaciPseudomonas amygdali pv. tabacie0pathogen
- Outcome
- global gene expression changese1
- Direction
- down-regulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“controlling of tobacco wildfire caused by Pseudomonas syringae pv. tabaci”
e1“The global gene expression changes of Pseudomonas syringae pv. tabaci supported that the functions of "protein secretion", "membrane part", "signal transducer activity", "locomotion", "chemotaxis" and "taxis" in bacteria, as well as the metabolic pathways of "bacterial chemotaxis", "two-component system", "biofilm formation", "ABC transporters" and "valine, leucine and isoleucine degradation" were significantly down-regulated by ZnO NPs”
Claim · model-written summary
ZnO NPs directly disrupted or suppressed the cell envelope structure, biofilm and motility of Pseudomonas syringae pv. tabaci.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- ZnO NPse2
- Interactor
- Pseudomonas syringae pv. tabaciPseudomonas amygdali pv. tabacie2pathogen
- Outcome
- cell envelope structure, biofilm and motilitye2
- Direction
- disrupted or suppressede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“controlling of tobacco wildfire caused by Pseudomonas syringae pv. tabaci”
e2“the cell envelope structure, biofilm and motility of Pseudomonas syringae pv. tabaci were directly disrupted or suppressed by ZnO NPs”
Claim · model-written summary
ZnO NPs at 0.5 mg/mL potentially improved plant growth and immunity of tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- ZnO NPse3
- Outcome
- plant growth and immunitye3
- Direction
- improvede3
- Value
- 0.5 mg/mLe3
- Qualifier
- potentiallye3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“controlling of tobacco wildfire caused by Pseudomonas syringae pv. tabaci”
e3“ZnO NPs (0.5 mg/mL) potentially improved plant growth and immunity”
Claim · model-written summary
ZnO NPs stimulated tobacco stomatal immunity, with ABA signalling and ABA concentration changes supporting this effect.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- ZnO NPse4
- Compound
- ABAe4
- Outcome
- stomatal immunitye4
- Direction
- stimulatee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“controlling of tobacco wildfire caused by Pseudomonas syringae pv. tabaci”
e4“the changes of gene expression in ABA signaling pathway, ABA concentration and stomatal aperture all supported that ZnO NPs can specifically stimulate stomatal immunity”
The same questions in other papers
nanoparticle · disease · Nicotiana tabacum (tobacco)Positive and negative results
9 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.