Abstract
Summary Using genetic resistance against bacterial blight (BB) caused by Xanthomonas oryzae pathovar oryzae ( Xoo ) is a major objective in rice breeding programmes. Prime editing (PE) has the potential to create novel germplasm against Xoo . Here, we use an improved prime‐editing system to implement two new strategies for BB resistance. Knock‐in of TAL effector binding elements (EBE) derived from the BB susceptible gene SWEET14 into the promoter of a dysfunctional executor R gene xa23 reaches 47.2% with desired edits including biallelic editing at 18% in T 0 generation that enables an inducible TALE‐dependent BB resistance. Editing the transcription factor TFIIA gene TFIIAγ5 required for TAL effector‐dependent BB susceptibility recapitulates the resistance of xa5 at an editing efficiency of 88.5% with biallelic editing rate of 30% in T 0 generation. The engineered loci provided resistance against multiple Xoo strains in T 1 generation. Whole‐genome sequencing detected no OsMLH1dn ‐associated random mutations and no off‐target editing demonstrating high specificity of this PE system. This is the first‐ever report to use PE system to engineer resistance against biotic stress and to demonstrate knock‐in of 30‐nucleotides cis‐regulatory element at high efficiency. The new strategies hold promises to fend rice off the evolving Xoo strains and protect it from epidemics.
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Claims 9
Claim · model-written summary
EBE knock in reached 47.2% desired editing.
- Taxon
- rice
Oryza sativat0 - Gene
- xa23e0
- Outcome
- desired editse0
- Value
- 47.2%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knock‐in of TAL effector binding elements (EBE) derived from the BB susceptible gene SWEET14 into the promoter of a dysfunctional executor R gene xa23 reaches 47.2% with desired edits”
t0“rice”
Claim · model-written summary
EBE knock in had 18% biallelic editing in T 0.
- Taxon
- riceOryza sativat0
- Organ / stage
- T 0 generatione0
- Outcome
- biallelic editinge0
- Value
- 18%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“biallelic editing at 18% in T 0 generation”
t0“rice”
Claim · model-written summary
EBE knock in enabled inducible TALE dependent BB resistance.
- Taxon
- riceOryza sativat0
- Treatment
- Knock‐in of TAL effector binding elements (EBE)e0
- Gene
- xa23e0
- Outcome
- inducible TALE‐dependent BB resistancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knock‐in of TAL effector binding elements (EBE) derived from the BB susceptible gene SWEET14 into the promoter of a dysfunctional executor R gene xa23 reaches 47.2% with desired edits including biallelic editing at 18% in T 0 generation that enables an inducible TALE‐dependent BB resistance”
t0“rice”
Claim · model-written summary
TFIIAγ5 editing reached 88.5% efficiency.
- Taxon
- riceOryza sativat0
- Gene
- TFIIAγ5e0
- Outcome
- editing efficiencye0
- Value
- 88.5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Editing the transcription factor TFIIA gene TFIIAγ5 required for TAL effector‐dependent BB susceptibility recapitulates the resistance of xa5 at an editing efficiency of 88.5%”
t0“rice”
Claim · model-written summary
TFIIAγ5 editing had 30% biallelic editing in T 0.
- Taxon
- riceOryza sativat0
- Outcome
- biallelic editing ratee0
- Value
- 30%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“biallelic editing rate of 30% in T 0 generation”
t0“rice”
Claim · model-written summary
TFIIAγ5 editing recapitulated xa5 resistance.
- Taxon
- riceOryza sativat0
- Treatment
- Editing the transcription factor TFIIA gene TFIIAγ5e0
- Gene
- TFIIAγ5e0
- Outcome
- resistance of xa5e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Editing the transcription factor TFIIA gene TFIIAγ5 required for TAL effector‐dependent BB susceptibility recapitulates the resistance of xa5”
t0“rice”
Claim · model-written summary
Engineered loci resisted multiple Xoo strains in T 1.
- Taxon
- riceOryza sativat0
- Interactor
- Xooe0pathogen
- Outcome
- resistance against multiple Xoo strainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The engineered loci provided resistance against multiple Xoo strains in T 1 generation”
t0“rice”
Claim · model-written summary
Sequencing found no OsMLH1dn associated random mutations.
- Taxon
- riceOryza sativat0
- Outcome
- no OsMLH1dn ‐associated random mutationse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Whole‐genome sequencing detected no OsMLH1dn ‐associated random mutations”
t0“rice”
Claim · model-written summary
Sequencing found no off target editing.
- Taxon
- riceOryza sativat0
- Outcome
- no off‐target editinge0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no off‐target editing demonstrating high specificity of this PE system”
t0“rice”
The same questions in other papers
pathogen product · disease · Oryza sativa (rice)Positive and negative results
14 papers in the same direction as this paper · 8 in the opposite direction
- negative
- no effect
- negative
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