Paper · 2023 · IOP Conference Series Earth and Environmental Science

Morphological and Molecular Diagnosis of Root-Knot Nematodes on Okra with Evaluation of the Efficacy of Olive, Sesame and Garlic Extract for Disease Control

Salih M. Ismail; Maher A. Khairi

11
claims
21
verbatim quotes
0
citations
3
research areas
✓
Open access · cc-by

Abstract

Abstract This study aimed to isolate and diagnose root knot nematodes on the okra plant based on the phenotypic diagnosis according to the perineal pattern and the polymerase chain reaction (PCR) method, and toevaluate the efficiency of olive stock, sesame, garlic extract, and the chemical pesticide Voual 24 SL in controlling the disease. The phenotypic diagnosis based on the perineal pattern showed that the diagnosed nematodes belong to Meliodogyen Incognita. The results of the Molecular diagnosis (PCR) were also confirmed by using the initiator Mel F/R to detect the S rDNA18 gene. The results of the partial diagnosis showed isolateion belonged to the nematode M. Incognita . A new Iraqi isolate was recorded in the global gene bank under number a (MW737418.1). The results of applying the following treatments Voual 24 SL, garlic extract, olive stock and sesame meal showed a reduction in complexity index and improvement in growth characteristics and plant yield. The Voual 24 SL pesticide and garlic extract treatment recorded a reduction in complexity index of 1.49, 1.83 and the highest plant height and plant yield recorded by the garlic extract treatment was 54.66 cm. The highest weight of wet roots was recorded in the treatment of sesame meal, which was 13.45 gm.

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Claims 11

Observationobservedoriginal resultmeasured

Claim · model-written summary

Perineal-pattern diagnosis identified the okra nematode as Meliodogyen Incognita.

Interactor
Meliodogyen Incognitae0pathogen
Outcome
diagnosed nematodes belong to Meliodogyen Incognitae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The phenotypic diagnosis based on the perineal pattern showed that the diagnosed nematodes belong to Meliodogyen Incognita.”

✓ located verbatim in the source · abstract, characters 341–464 · show in text ↑
t0

“on Okra”

✓ located verbatim in the source · title, characters 61–68 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

PCR with Mel F/R detected the S rDNA18 gene and supported identification of the okra nematode isolate as M. Incognita.

Interactor
M. Incognitae0pathogen
Gene
S rDNA18e0
Outcome
detect the S rDNA18 genee0

Not reportedComparator

Verbatim

e0

“The results of the Molecular diagnosis (PCR) were also confirmed by using the initiator Mel F/R to detect the S rDNA18 gene. The results of the partial diagnosis showed isolateion belonged to the nematode M. Incognita .”

✓ located verbatim in the source · abstract, characters 465–684 · show in text ↑
Method / resourceobservedoriginal resultbasis n/a

Claim · model-written summary

A new Iraqi M. Incognita isolate was registered under MW737418.1.

Interactor
Root-Knot NematodesMeloidogynet0pathogen
Outcome
new Iraqi isolatee0
Value
MW737418.1e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Meloidogyne
Meloidogyne · Interactor · picture of the genus (one of its species)Photo by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“A new Iraqi isolate was recorded in the global gene bank under number a (MW737418.1).”

✓ located verbatim in the source · abstract, characters 685–770 · show in text ↑
t0

“Root-Knot Nematodes on Okra”

✓ located verbatim in the source · title, characters 41–68 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Voual 24 SL reduced the okra nematode complexity index.

Treatment
Voual 24 SLe0
Interactor
Root-Knot NematodesMeloidogynet0pathogen
Outcome
complexity indexe0
Direction
reductione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Meloidogyne
Meloidogyne · Interactor · picture of the genus (one of its species)Photo by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The results of applying the following treatments Voual 24 SL, garlic extract, olive stock and sesame meal showed a reduction in complexity index and improvement in growth characteristics and plant yield.”

✓ located verbatim in the source · abstract, characters 771–974 · show in text ↑
t0

“Root-Knot Nematodes on Okra”

✓ located verbatim in the source · title, characters 41–68 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Garlic extract reduced the okra nematode complexity index.

Treatment
garlic extracte0
Interactor
Root-Knot NematodesMeloidogynet0pathogen
Outcome
complexity indexe0
Direction
reductione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Meloidogyne
Meloidogyne · Interactor · picture of the genus (one of its species)Photo by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The results of applying the following treatments Voual 24 SL, garlic extract, olive stock and sesame meal showed a reduction in complexity index and improvement in growth characteristics and plant yield.”

✓ located verbatim in the source · abstract, characters 771–974 · show in text ↑
t0

“Root-Knot Nematodes on Okra”

✓ located verbatim in the source · title, characters 41–68 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Olive stock reduced the okra nematode complexity index.

Treatment
olive stocke0
Interactor
Root-Knot NematodesMeloidogynet0pathogen
Outcome
complexity indexe0
Direction
reductione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Meloidogyne
Meloidogyne · Interactor · picture of the genus (one of its species)Photo by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The results of applying the following treatments Voual 24 SL, garlic extract, olive stock and sesame meal showed a reduction in complexity index and improvement in growth characteristics and plant yield.”

✓ located verbatim in the source · abstract, characters 771–974 · show in text ↑
t0

“Root-Knot Nematodes on Okra”

✓ located verbatim in the source · title, characters 41–68 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sesame meal reduced the okra nematode complexity index.

Treatment
sesame meale0
Interactor
Root-Knot NematodesMeloidogynet0pathogen
Outcome
complexity indexe0
Direction
reductione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Meloidogyne
Meloidogyne · Interactor · picture of the genus (one of its species)Photo by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The results of applying the following treatments Voual 24 SL, garlic extract, olive stock and sesame meal showed a reduction in complexity index and improvement in growth characteristics and plant yield.”

✓ located verbatim in the source · abstract, characters 771–974 · show in text ↑
t0

“Root-Knot Nematodes on Okra”

✓ located verbatim in the source · title, characters 41–68 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Voual 24 SL and garlic extract recorded complexity indices of 1.49 and 1.83, respectively, in treated okra.

Treatment
Voual 24 SL pesticide and garlic extract treatmente0
Outcome
complexity indexe0
Direction
reductione0
Value
1.49, 1.83e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The Voual 24 SL pesticide and garlic extract treatment recorded a reduction in complexity index of 1.49, 1.83”

✓ located verbatim in the source · abstract, characters 975–1084 · show in text ↑
t0

“on Okra”

✓ located verbatim in the source · title, characters 61–68 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Garlic extract gave the highest recorded okra plant height, 54.66 cm.

Treatment
garlic extract treatmente0
Outcome
plant heighte0
Direction
higheste0
Value
54.66 cme0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the highest plant height and plant yield recorded by the garlic extract treatment was 54.66 cm”

✓ located verbatim in the source · abstract, characters 1089–1183 · show in text ↑
t0

“on Okra”

✓ located verbatim in the source · title, characters 61–68 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Garlic extract produced the highest recorded okra plant yield.

Treatment
garlic extract treatmente0
Outcome
plant yielde0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the highest plant height and plant yield recorded by the garlic extract treatment was 54.66 cm”

✓ located verbatim in the source · abstract, characters 1089–1183 · show in text ↑
t0

“on Okra”

✓ located verbatim in the source · title, characters 61–68 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sesame meal produced the highest recorded wet root weight in okra, 13.45 gm.

Treatment
sesame meale0
Outcome
weight of wet rootse0
Direction
higheste0
Value
13.45 gme0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest weight of wet roots was recorded in the treatment of sesame meal, which was 13.45 gm.”

✓ located verbatim in the source · abstract, characters 1185–1282 · show in text ↑
t0

“on Okra”

✓ located verbatim in the source · title, characters 61–68 · show in text ↑

Allium sativum · growth · Abelmoschus esculentus (okra)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Allium sativum · yield · Abelmoschus esculentus (okra)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

botanical · yield · Abelmoschus esculentus (okra)Results differ

78 papers: 69 positive, 9 negative or no effect

68 papers in the same direction as this paper · 5 in the opposite direction · 4 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

74 papers more in this group

See all 78 papers →

botanical · growth · Abelmoschus esculentus (okra)Positive and negative results

57 papers: 49 positive, 8 negative or no effect

48 papers in the same direction as this paper · 8 in the opposite direction

  1. negative
  2. negative
  3. negative

53 papers more in this group

See all 57 papers →

botanical · Meloidogyne · Abelmoschus esculentus (okra)Results agree

13 papers: 13 positive, 0 negative or no effect

12 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

9 papers more in this group

See all 13 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.