Abstract
Sugarcane is an important sugar and bioenergy source and a significant component of the economy in various countries in arid and semiarid. It requires more synthetic fertilizers and fungicides during growth and development. However, the excess use of synthetic fertilizers and fungicides causes environmental pollution and affects cane quality and productivity. Plant growth-promoting bacteria (PGPB) indirectly or directly promote plant growth in various ways. In this study, 22 PGPB strains were isolated from the roots of the sugarcane variety GT42. After screening of plant growth-promoting (PGP) traits, it was found that the DJ06 strain had the most potent PGP activity, which was identified as Pseudomonas aeruginosa by 16S rRNA gene sequencing. Scanning electron microscopy (SEM) and green fluorescent protein (GFP) labeling technology confirmed that the DJ06 strain successfully colonized sugarcane tissues. The complete genome sequencing of the DJ06 strain was performed using Nanopore and Illumina sequencing platforms. The results showed that the DJ06 strain genome size was 64,90,034 bp with a G+C content of 66.34%, including 5,912 protein-coding genes (CDSs) and 12 rRNA genes. A series of genes related to plant growth promotion was observed, such as nitrogen fixation, ammonia assimilation, siderophore, 1-aminocyclopropane-1-carboxylic acid (ACC), deaminase, indole-3-acetic acid (IAA) production, auxin biosynthesis, phosphate metabolism, hydrolase, biocontrol, and tolerance to abiotic stresses. In addition, the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8. The length of the plant was increased significantly by 32.43 and 12.66% and fresh weight by 89.87 and 135.71% in sugarcane GT11 and B8 at 60 days after inoculation. The photosynthetic leaf gas exchange also increased significantly compared with the control plants. The content of indole-3-acetic acid (IAA) was enhanced and gibberellins (GA) and abscisic acid (ABA) were reduced in response to inoculation of the DJ06 strain as compared with control in two sugarcane varieties. The enzymatic activities of oxidative, nitrogen metabolism, and hydrolases were also changed dramatically in both sugarcane varieties with inoculation of the DJ06 strain. These findings provide better insights into the interactive action mechanisms of the P. aeruginosa DJ06 strain and sugarcane plant development.
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Claims 18
Claim · model-written summary
Inoculation with the DJ06 strain increased plant length of sugarcane GT11 by 32.43% at 60 days after inoculation.
- Taxon
- sugarcanee1
- Material
- GT11e1
- Treatment
- DJ06 straine1
- Interactor
- Pseudomonas aeruginosa DJ06
Pseudomonas aeruginosat0symbiont - Outcome
- length of the plante2
- Direction
- increasede2
- Value
- 32.43e2
- Qualifier
- at 60 days after inoculatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
e2“The length of the plant was increased significantly by 32.43 and 12.66% and fresh weight by 89.87 and 135.71% in sugarcane GT11 and B8 at 60 days after inoculation”
Claim · model-written summary
Inoculation with the DJ06 strain increased fresh weight of sugarcane GT11 by 89.87% at 60 days after inoculation.
- Taxon
- sugarcanee1
- Material
- GT11e1
- Treatment
- DJ06 straine1
- Interactor
- Pseudomonas aeruginosa DJ06Pseudomonas aeruginosat0symbiont
- Outcome
- fresh weighte2
- Direction
- increasede2
- Value
- 89.87e2
- Qualifier
- at 60 days after inoculatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
e2“The length of the plant was increased significantly by 32.43 and 12.66% and fresh weight by 89.87 and 135.71% in sugarcane GT11 and B8 at 60 days after inoculation”
Claim · model-written summary
Inoculation with the DJ06 strain increased plant length of sugarcane B8 by 12.66% at 60 days after inoculation.
- Taxon
- sugarcanee1
- Material
- B8e1
- Treatment
- DJ06 straine1
- Interactor
- Pseudomonas aeruginosa DJ06Pseudomonas aeruginosat0symbiont
- Outcome
- length of the plante2
- Direction
- increasede2
- Value
- 12.66%e2
- Qualifier
- at 60 days after inoculatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
e2“The length of the plant was increased significantly by 32.43 and 12.66% and fresh weight by 89.87 and 135.71% in sugarcane GT11 and B8 at 60 days after inoculation”
Claim · model-written summary
Inoculation with the DJ06 strain increased fresh weight of sugarcane B8 by 135.71% at 60 days after inoculation.
- Taxon
- sugarcanee1
- Material
- B8e1
- Treatment
- DJ06 straine1
- Interactor
- Pseudomonas aeruginosa DJ06Pseudomonas aeruginosat0symbiont
- Outcome
- fresh weighte2
- Direction
- increasede2
- Value
- 135.71%e2
- Qualifier
- at 60 days after inoculatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
e2“The length of the plant was increased significantly by 32.43 and 12.66% and fresh weight by 89.87 and 135.71% in sugarcane GT11 and B8 at 60 days after inoculation”
Claim · model-written summary
Inoculation with the DJ06 strain significantly increased photosynthetic leaf gas exchange compared with control plants.
Verbatim
e3“The photosynthetic leaf gas exchange also increased significantly compared with the control plants”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
Claim · model-written summary
Inoculation with the DJ06 strain enhanced indole-3-acetic acid (IAA) content in sugarcane compared with control.
Verbatim
e4“The content of indole-3-acetic acid (IAA) was enhanced and gibberellins (GA) and abscisic acid (ABA) were reduced in response to inoculation of the DJ06 strain as compared with control in two sugarcane varieties”
Claim · model-written summary
Inoculation with the DJ06 strain reduced gibberellins (GA) and abscisic acid (ABA) content in sugarcane compared with control.
Verbatim
e4“The content of indole-3-acetic acid (IAA) was enhanced and gibberellins (GA) and abscisic acid (ABA) were reduced in response to inoculation of the DJ06 strain as compared with control in two sugarcane varieties”
Claim · model-written summary
Inoculation with the DJ06 strain changed the enzymatic activities of oxidative, nitrogen metabolism and hydrolases dramatically in both sugarcane varieties.
- Taxon
- sugarcanee5
- Treatment
- inoculation of the DJ06 straine5
- Outcome
- enzymatic activities of oxidative, nitrogen metabolism, and hydrolasese5
- Direction
- changed dramaticallye5
Not reportedComparator
Verbatim
e5“The enzymatic activities of oxidative, nitrogen metabolism, and hydrolases were also changed dramatically in both sugarcane varieties with inoculation of the DJ06 strain”
Claim · model-written summary
The DJ06 strain genome size is 64,90,034 bp.
- Taxon
- sugarcanee1
- Interactor
- Pseudomonas aeruginosa DJ06Pseudomonas aeruginosat0symbiont
- Outcome
- DJ06 strain genome sizee6
- Value
- 64,90,034 bpe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The results showed that the DJ06 strain genome size was 64,90,034 bp with a G+C content of 66.34%, including 5,912 protein-coding genes (CDSs) and 12 rRNA genes”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
Claim · model-written summary
The DJ06 strain genome has a G+C content of 66.34%.
- Taxon
- sugarcanee1
- Interactor
- Pseudomonas aeruginosa DJ06Pseudomonas aeruginosat0symbiont
- Outcome
- G+C contente6
- Value
- 66.34%e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The results showed that the DJ06 strain genome size was 64,90,034 bp with a G+C content of 66.34%, including 5,912 protein-coding genes (CDSs) and 12 rRNA genes”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
Claim · model-written summary
The DJ06 strain genome contains 5,912 protein-coding genes (CDSs).
- Taxon
- sugarcanee1
- Interactor
- Pseudomonas aeruginosa DJ06Pseudomonas aeruginosat0symbiont
- Outcome
- protein-coding genes (CDSs)e6
- Value
- 5,912e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The results showed that the DJ06 strain genome size was 64,90,034 bp with a G+C content of 66.34%, including 5,912 protein-coding genes (CDSs) and 12 rRNA genes”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
Claim · model-written summary
The DJ06 strain genome contains 12 rRNA genes.
- Taxon
- sugarcanee1
- Interactor
- Pseudomonas aeruginosa DJ06Pseudomonas aeruginosat0symbiont
- Outcome
- rRNA genese6
- Value
- 12e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The results showed that the DJ06 strain genome size was 64,90,034 bp with a G+C content of 66.34%, including 5,912 protein-coding genes (CDSs) and 12 rRNA genes”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
Claim · model-written summary
Genes related to plant growth promotion were found in the DJ06 strain for nitrogen fixation, ammonia assimilation and siderophore production.
- Taxon
- sugarcanee1
- Interactor
- Pseudomonas aeruginosa DJ06Pseudomonas aeruginosat0symbiont
- Outcome
- genes related to plant growth promotione7
- Qualifier
- nitrogen fixation, ammonia assimilation, siderophoree7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“A series of genes related to plant growth promotion was observed, such as nitrogen fixation, ammonia assimilation, siderophore, 1-aminocyclopropane-1-carboxylic acid (ACC), deaminase, indole-3-acetic acid (IAA) production, auxin biosynthesis, phosphate metabolism, hydrolase, biocontrol, and tolerance to abiotic stresses”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
Claim · model-written summary
Genes related to plant growth promotion were found in the DJ06 strain for ACC deaminase, IAA production and auxin biosynthesis.
- Taxon
- sugarcanee1
- Interactor
- Pseudomonas aeruginosa DJ06Pseudomonas aeruginosat0symbiont
- Outcome
- genes related to plant growth promotione7
- Qualifier
- deaminase, indole-3-acetic acid (IAA) production, auxin biosynthesise7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“A series of genes related to plant growth promotion was observed, such as nitrogen fixation, ammonia assimilation, siderophore, 1-aminocyclopropane-1-carboxylic acid (ACC), deaminase, indole-3-acetic acid (IAA) production, auxin biosynthesis, phosphate metabolism, hydrolase, biocontrol, and tolerance to abiotic stresses”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
Claim · model-written summary
Genes related to plant growth promotion were found in the DJ06 strain for phosphate metabolism, hydrolase and biocontrol.
- Taxon
- sugarcanee1
- Interactor
- Pseudomonas aeruginosa DJ06Pseudomonas aeruginosat0symbiont
- Outcome
- genes related to plant growth promotione7
- Qualifier
- phosphate metabolism, hydrolase, biocontrole7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“A series of genes related to plant growth promotion was observed, such as nitrogen fixation, ammonia assimilation, siderophore, 1-aminocyclopropane-1-carboxylic acid (ACC), deaminase, indole-3-acetic acid (IAA) production, auxin biosynthesis, phosphate metabolism, hydrolase, biocontrol, and tolerance to abiotic stresses”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
Claim · model-written summary
Genes related to plant growth promotion were found in the DJ06 strain for tolerance to abiotic stresses.
- Taxon
- sugarcanee1
- Interactor
- Pseudomonas aeruginosa DJ06Pseudomonas aeruginosat0symbiont
- Outcome
- genes related to plant growth promotione7
- Qualifier
- tolerance to abiotic stressese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“A series of genes related to plant growth promotion was observed, such as nitrogen fixation, ammonia assimilation, siderophore, 1-aminocyclopropane-1-carboxylic acid (ACC), deaminase, indole-3-acetic acid (IAA) production, auxin biosynthesis, phosphate metabolism, hydrolase, biocontrol, and tolerance to abiotic stresses”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
Claim · model-written summary
The DJ06 strain colonized sugarcane tissues, confirmed by scanning electron microscopy and green fluorescent protein labeling.
- Taxon
- sugarcanee0
- Interactor
- Pseudomonas aeruginosa DJ06Pseudomonas aeruginosat0symbiont
- Outcome
- colonized sugarcane tissuese0
- Qualifier
- Scanning electron microscopy (SEM) and green fluorescent protein (GFP) labelinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was found that the DJ06 strain had the most potent PGP activity, which was identified as Pseudomonas aeruginosa by 16S rRNA gene sequencing. Scanning electron microscopy (SEM) and green fluorescent protein (GFP) labeling technology confirmed that the DJ06 strain successfully colonized sugarcane tissues”
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
Claim · model-written summary
The DJ06 strain had the most potent plant growth-promoting activity among the screened isolates.
- Taxon
- sugarcanee1
- Interactor
- Pseudomonas aeruginosa DJ06Pseudomonas aeruginosat0symbiont
- Outcome
- PGP activitye0
- Qualifier
- the most potente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was found that the DJ06 strain had the most potent PGP activity, which was identified as Pseudomonas aeruginosa by 16S rRNA gene sequencing. Scanning electron microscopy (SEM) and green fluorescent protein (GFP) labeling technology confirmed that the DJ06 strain successfully colonized sugarcane tissues”
e1“the effect of the DJ06 strain was also evaluated by inoculation in two sugarcane varieties GT11 and B8”
t0“endophytic diazotroph Pseudomonas aeruginosa DJ06”
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