Abstract
Ethylene plays an important role in grape ripening, acting through ethylene response factors (ERFs) that are downstream of ethylene signaling pathways, yet little else is known about these molecular mechanisms. In our study, we demonstrate that ethylene treatment promotes grape ripening and that the transcription factor VvERF75 shows increased expression during grape ripening. VvERF75 was found to contain a conserved AP2/ERF domain, to be a member of the ERF IXa subfamily, an to localize to the nucleus and to have transactivation activity in yeast. Our in vitro assays showed that VvERF75 can positively regulate expression of pheophorbide a oxygenase (VvPAO1) and 1-aminocyclopropane-1-carboxylic acid synthase (VvACS5) by binding to DRE motifs in their promoters. Overexpression of VvERF75 in tomato increased ethylene production and resulted in dwarfed plants, early flowering and fruit ripening, increased SlPAO and SlACS transcript levels, and decreased chlorophyll content. In general, our study revealed that VvERF75 may regulate fruit ripening by promoting ethylene biosynthesis and chlorophyll degradation, a discovery that lays a foundation for deciphering the molecular mechanisms of grape ripening.
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Claims 12
Claim · model-written summary
Ethylene treatment promoted grape ripening.
Not reportedComparator
Verbatim
e0“ethylene treatment promotes grape ripening”
Claim · model-written summary
VvERF75 expression rose during grape ripening.
Verbatim
e0“the transcription factor VvERF75 shows increased expression during grape ripening.”
Claim · model-written summary
VvERF75 contained a conserved AP2/ERF domain and belonged to the ERF IXa subfamily.
Verbatim
e0“VvERF75 was found to contain a conserved AP2/ERF domain, to be a member of the ERF IXa subfamily”
t0“grape”
Claim · model-written summary
VvERF75 localized to the nucleus and showed transactivation activity in yeast.
Verbatim
e0“to localize to the nucleus and to have transactivation activity in yeast.”
t0“grape”
Claim · model-written summary
VvERF75 positively regulated VvPAO1 expression by promoter binding in vitro.
Verbatim
e0“VvERF75 can positively regulate expression of pheophorbide a oxygenase (VvPAO1) and 1-aminocyclopropane-1-carboxylic acid synthase (VvACS5) by binding to DRE motifs in their promoters.”
t0“grape”
Claim · model-written summary
VvERF75 positively regulated VvACS5 expression by promoter binding in vitro.
Verbatim
e0“VvERF75 can positively regulate expression of pheophorbide a oxygenase (VvPAO1) and 1-aminocyclopropane-1-carboxylic acid synthase (VvACS5) by binding to DRE motifs in their promoters.”
t0“grape”
Claim · model-written summary
VvERF75 overexpression increased ethylene production in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- Overexpression of VvERF75e0
- Gene
- VvERF75e0
- Outcome
- ethylene productione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of VvERF75 in tomato increased ethylene production”
Claim · model-written summary
VvERF75 overexpression caused dwarfing in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Overexpression of VvERF75e0
- Gene
- VvERF75e0
- Outcome
- dwarfed plantse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of VvERF75 in tomato increased ethylene production and resulted in dwarfed plants, early flowering and fruit ripening”
Claim · model-written summary
VvERF75 overexpression advanced flowering and fruit ripening in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Overexpression of VvERF75e0
- Gene
- VvERF75e0
- Outcome
- early flowering and fruit ripeninge0
- Direction
- earlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of VvERF75 in tomato increased ethylene production and resulted in dwarfed plants, early flowering and fruit ripening”
Claim · model-written summary
VvERF75 overexpression increased SlPAO and SlACS transcripts in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Overexpression of VvERF75e0
- Gene
- VvERF75e0
- Outcome
- SlPAO and SlACS transcript levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of VvERF75 in tomato increased ethylene production and resulted in dwarfed plants, early flowering and fruit ripening, increased SlPAO and SlACS transcript levels”
Claim · model-written summary
VvERF75 overexpression decreased chlorophyll content in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Overexpression of VvERF75e0
- Gene
- VvERF75e0
- Outcome
- chlorophyll contente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of VvERF75 in tomato increased ethylene production and resulted in dwarfed plants, early flowering and fruit ripening, increased SlPAO and SlACS transcript levels, and decreased chlorophyll content.”
Claim · model-written summary
The authors suggest VvERF75 may regulate grape fruit ripening through ethylene biosynthesis and chlorophyll degradation.
Verbatim
e0“VvERF75 may regulate fruit ripening by promoting ethylene biosynthesis and chlorophyll degradation”
t0“grape”
The same questions in other papers
ethene · quality: postharvest · VitisPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.