Abstract
KEY MESSAGE: GRF-GIF chimeric proteins from multiple source species enhance in vitro regeneration in both wild and cultivated lettuce. In addition, they enhance regeneration in multiple types of lettuce including butterheads, romaines, and crispheads. The ability of plants to regenerate in vitro has been exploited for use in tissue culture systems for plant propagation, plant transformation, and genome editing. The success of in vitro regeneration is often genotype dependent and continues to be a bottleneck for Agrobacterium-mediated transformation and its deployment for improvement of some crop species. Manipulation of transcription factors that play key roles in plant development such as BABY BOOM, WUSCHEL, and GROWTH-REGULATING FACTORs (GRFs) has improved regeneration and transformation efficiencies in several plant species. Here, we compare the efficacy of GRF-GIF gene fusions from multiple species to boost regeneration efficiency and shooting frequency in four genotypes of wild and cultivated lettuce (Lactuca spp. L.). In addition, we show that GRF-GIFs with mutated miRNA 396 binding sites increase regeneration efficiency and shooting frequency when compared to controls. We also present a co-transformation strategy for increased transformation efficiency and recovery of transgenic plants harboring a gene of interest. This strategy will enhance the recovery of transgenic plants of other lettuce genotypes and likely other crops in the Compositae family.
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Claims 4
Claim · model-written summary
GRF-GIF chimeric proteins from multiple species enhance regeneration in wild and cultivated lettuce.
- Taxon
- lettuce
Lactuca sativae0 - Treatment
- GRF-GIF chimeric proteins from multiple source speciese0
- Outcome
- in vitro regeneratione0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GRF-GIF chimeric proteins from multiple source species enhance in vitro regeneration in both wild and cultivated lettuce”
Claim · model-written summary
GRF-GIF chimeric proteins enhance regeneration in butterhead, romaine and crisphead lettuce.
- Taxon
- lettuceLactuca sativat0
- Material
- butterheads, romaines, and crispheadse0
- Treatment
- GRF-GIF chimeric proteins from multiple source speciese1
- Outcome
- regeneratione0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they enhance regeneration in multiple types of lettuce including butterheads, romaines, and crispheads”
t0“lettuce (Lactuca spp.)”
e1“GRF-GIF chimeric proteins from multiple source species”
Claim · model-written summary
GRF-GIF constructs with mutated miRNA 396 binding sites increased lettuce regeneration efficiency and shooting frequency relative to controls.
- Taxon
- lettuceLactuca sativat0
- Treatment
- GRF-GIFs with mutated miRNA 396 binding sitese0
- Gene
- miRNA 396e0
- Outcome
- regeneration efficiency and shooting frequencye0
- Direction
- increasee0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GRF-GIFs with mutated miRNA 396 binding sites increase regeneration efficiency and shooting frequency when compared to controls”
t0“lettuce (Lactuca spp.)”
Claim · model-written summary
The co-transformation strategy increased transformation efficiency and recovery of transgenic lettuce plants.
- Taxon
- lettuceLactuca sativat0
- Outcome
- transformation efficiency and recovery of transgenic plantse0
- Direction
- increasede0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a co-transformation strategy for increased transformation efficiency and recovery of transgenic plants harboring a gene of interest”
t0“lettuce (Lactuca spp.)”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.