Abstract
) calli to UV-B radiation and performed a transcriptome deep sequencing (RNA-seq) analysis to identify differentially expressed genes (DEGs). We detected 16,899 DEGs among different treatments, with the largest number seen after 24 h of UV-B exposure relative to controls. Functional annotation and enrichment analysis showed a significant enrichment for DEGs in pathways related to plant hormone signal transduction and phenylpropanoid and flavonoid biosynthesis. In agreement with the transcriptome data, flavonol, anthocyanin and proanthocyanidin accumulated upon UV-B radiation, and most DEGs mapping to the phenylpropanoid and flavonoid biosynthetic pathways using the KEGG mapper tool were upregulated under UV-B radiation. We also performed a weighted gene co-expression network analysis (WGCNA) to explore the relationship among genes involved in plant hormone signal transduction, encoding transcription factors or participating in flavonoid biosynthesis. The transcription factors VcMYBPA1, MYBPA2.1, MYB114, MYBA2, MYBF, and MYB102 are likely activators, whereas MYB20, VcMYB14, MYB44, and VcMYB4a are inhibitors of the flavonoid biosynthetic pathway, as evidenced by the direction of correlation between the expression of these MYBs and flavonoid biosynthesis-related genes. The transcription factors bHLH74 and bHLH25 might interact with MYB repressors or directly inhibited the expression of flavonoid biosynthetic genes to control flavonoid accumulation. We also observed the downregulation of several genes belonging to the auxin, gibberellin and brassinosteroid biosynthetic pathways, suggesting that MYB inhibitors or activators are directly or indirectly regulated to promote flavonoid biosynthesis under UV-B radiation.
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Claims 9
Claim · model-written summary
UV-B treatments yielded 16,899 differentially expressed genes, most after 24 h.
Verbatim
t0“blueberry (Vaccinium corymbosum)”
e0“We detected 16,899 DEGs among different treatments, with the largest number seen after 24 h of UV-B exposure relative to controls.”
Claim · model-written summary
Differentially expressed genes were enriched in hormone signal transduction and phenylpropanoid and flavonoid biosynthesis.
Verbatim
t0“blueberry (Vaccinium corymbosum)”
e0“Functional annotation and enrichment analysis showed a significant enrichment for DEGs in pathways related to plant hormone signal transduction and phenylpropanoid and flavonoid biosynthesis.”
Claim · model-written summary
Flavonol, anthocyanin and proanthocyanidin accumulated under UV-B.
- Taxon
- blueberryt0
- Treatment
- UV-B radiatione0
- Outcome
- flavonol, anthocyanin and proanthocyanidine0
- Direction
- accumulatede0
Not reportedComparator
Verbatim
t0“blueberry (Vaccinium corymbosum)”
e0“flavonol, anthocyanin and proanthocyanidin accumulated upon UV-B radiation”
Claim · model-written summary
Most phenylpropanoid and flavonoid pathway DEGs were upregulated under UV-B.
- Taxon
- blueberryt0
- Treatment
- UV-B radiatione0
- Outcome
- DEGs mapping to the phenylpropanoid and flavonoid biosynthetic pathwayse0
- Direction
- upregulatede0
Not reportedComparator
Verbatim
t0“blueberry (Vaccinium corymbosum)”
e0“most DEGs mapping to the phenylpropanoid and flavonoid biosynthetic pathways using the KEGG mapper tool were upregulated under UV-B radiation.”
Claim · model-written summary
MYB factors were associated with activation of flavonoid biosynthesis.
Verbatim
t0“blueberry (Vaccinium corymbosum)”
e0“The transcription factors VcMYBPA1, MYBPA2.1, MYB114, MYBA2, MYBF, and MYB102 are likely activators, whereas MYB20, VcMYB14, MYB44, and VcMYB4a are inhibitors of the flavonoid biosynthetic pathway, as evidenced by the direction of correlation between the expression of these MYBs and flavonoid biosynthesis-related genes.”
Claim · model-written summary
MYB factors were associated with inhibition of flavonoid biosynthesis.
Verbatim
t0“blueberry (Vaccinium corymbosum)”
e0“The transcription factors VcMYBPA1, MYBPA2.1, MYB114, MYBA2, MYBF, and MYB102 are likely activators, whereas MYB20, VcMYB14, MYB44, and VcMYB4a are inhibitors of the flavonoid biosynthetic pathway, as evidenced by the direction of correlation between the expression of these MYBs and flavonoid biosynthesis-related genes.”
Claim · model-written summary
bHLH74 and bHLH25 may inhibit flavonoid biosynthetic genes and control flavonoid accumulation.
Verbatim
t0“blueberry (Vaccinium corymbosum)”
e0“The transcription factors bHLH74 and bHLH25 might interact with MYB repressors or directly inhibited the expression of flavonoid biosynthetic genes to control flavonoid accumulation.”
Claim · model-written summary
Several auxin, gibberellin and brassinosteroid pathway genes were downregulated.
Verbatim
t0“blueberry (Vaccinium corymbosum)”
e0“We also observed the downregulation of several genes belonging to the auxin, gibberellin and brassinosteroid biosynthetic pathways”
Claim · model-written summary
The authors suggest MYB regulators promote flavonoid biosynthesis under UV-B.
Verbatim
t0“blueberry (Vaccinium corymbosum)”
e0“suggesting that MYB inhibitors or activators are directly or indirectly regulated to promote flavonoid biosynthesis under UV-B radiation.”
The same questions in other papers
uv · quality: phytochemicals · VacciniumPositive and negative results
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- positive
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.