Abstract
The plant glutathione S-transferases (GSTs) are versatile proteins encoded by several genes and play vital roles in responding to various physiological processes. Members of plant GSTs have been identified in several species, but few studies on cucumber ( Cucumis sativus L.) have been reported. In this study, we identified 46 GST genes, which were divided into 11 classes. Chromosomal location and genome mapping revealed that cucumber GSTs (CsGSTs) were unevenly distributed in seven chromosomes, and the syntenic regions differed in each chromosome. The conserved motifs and gene structure of CsGSTs were analyzed using MEME and GSDS 2.0 online tools, respectively. Transcriptome and RT-qPCR analysis revealed that most CsGST members responded to cold stress. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses for differentially expressed CsGSTs under cold stress revealed that these genes responded to cold stress probably through “glutathione metabolism.” Finally, we screened seven candidates that may be involved in cold stress using Venn analysis, and their promoters were analyzed using PlantCARE and New PLACE tools to predict the factors regulating these genes. Antioxidant enzyme activities were increased under cold stress conditions, which conferred tolerance against cold stress. Our study illustrates the characteristics and functions of CsGST genes, especially in responding to cold stress in cucumber.
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Claims 8
Claim · model-written summary
Forty-six cucumber GST genes were identified and divided into eleven classes.
- Taxon
- cucumber
Cucumis sativust0 - Gene
- GST genese0
- Outcome
- GST genese0
- Value
- 46e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified 46 GST genes, which were divided into 11 classes”
t0“cucumber”
Claim · model-written summary
Cucumber GSTs were unevenly distributed across seven chromosomes.
- Taxon
- cucumberCucumis sativuse0
- Gene
- CsGSTse0
- Outcome
- unevenly distributed in seven chromosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cucumber GSTs (CsGSTs) were unevenly distributed in seven chromosomes”
Claim · model-written summary
Syntenic regions differed by cucumber chromosome.
- Taxon
- cucumberCucumis sativust0
- Outcome
- syntenic regions differed in each chromosomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the syntenic regions differed in each chromosome”
t0“cucumber”
Claim · model-written summary
Most cucumber CsGST members responded to cold stress.
- Taxon
- cucumberCucumis sativust0
- Gene
- CsGSTe0
- Outcome
- responded to cold stresse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most CsGST members responded to cold stress”
t0“cucumber”
Claim · model-written summary
Differentially expressed cucumber CsGSTs probably responded to cold stress through glutathione metabolism.
- Taxon
- cucumberCucumis sativust0
- Gene
- CsGSTse0
- Outcome
- responded to cold stresse0
- Qualifier
- glutathione metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differentially expressed CsGSTs under cold stress revealed that these genes responded to cold stress probably through “glutathione metabolism.””
t0“cucumber”
Claim · model-written summary
Seven cucumber candidate genes potentially involved in cold stress were screened.
- Taxon
- cucumberCucumis sativust0
- Outcome
- candidates that may be involved in cold stresse0
- Value
- sevene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we screened seven candidates that may be involved in cold stress using Venn analysis”
t0“cucumber”
Claim · model-written summary
Antioxidant enzyme activities increased in cucumber under cold stress.
- Taxon
- cucumberCucumis sativust0
- Treatment
- cold stress conditionse0
- Outcome
- Antioxidant enzyme activitiese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antioxidant enzyme activities were increased under cold stress conditions”
t0“cucumber”
Claim · model-written summary
Increased antioxidant enzyme activities conferred cucumber cold tolerance, according to the authors.
- Taxon
- cucumberCucumis sativust0
- Outcome
- tolerance against cold stresse0
- Qualifier
- Antioxidant enzyme activitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antioxidant enzyme activities were increased under cold stress conditions, which conferred tolerance against cold stress”
t0“cucumber”
The same questions in other papers
cold · soil: biology · Cucumis sativus (cucumber)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.