Abstract
The crude extract of phytase from rice bran was prepared by the extraction with 2.0% CaCl2 solution and partially purified by using of ammonium sulphate (35-80% saturation) followed by the dialysis in distilled water. The specific activity of enzyme was increased by 7.4 and 10.8 folds after ammonium sulphate fractionation and dialysis, respectively comparing with the crude extract. Characteristics of the partially purified rice bran phytase was also studied. The enzyme showed an optimum pH of 5.0 and optimum temperature of 50°C. Data concerning the rate of thermal inactivation revealed that the enzyme retained about 80% and 30% of its activity when heated for 10 min. at 60 and 70°C, respectively. The enzyme activity was reduced at high substrate concentrations with an optimum substrate concentration of 3.0 mM sodium phytate. The Km value was found to be 1.66x10-3 M and the Vmax was 0.95 µM Pi liberated/min/ml enzyme. The enzyme activity was markedly influenced by the presence of some metal salts. Each of CoCl2, MnSo4, CaCl2 and MgSO4 showed an activating effect on the enzyme reaction when added at concentrations of 1x10-4 or 1x10-3 M. However, both ZnSO4 and NiCl2 caused partial inhibitory effect, while FeSO4 and CuSO4 were found to strongly inhibit the enzyme activity.
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Claims 13
Claim · model-written summary
Ammonium sulphate fractionation increased phytase specific activity 7.4-fold over crude extract.
- Taxon
- RICE
Oryza sativat0 - Treatment
- ammonium sulphate fractionatione0
- Gene
- enzymee0
- Outcome
- specific activity of enzymee0
- Direction
- increasede0
- Value
- 7.4e0
- Comparator
- crude extracte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE BRAN”
e0“The specific activity of enzyme was increased by 7.4 and 10.8 folds after ammonium sulphate fractionation and dialysis, respectively comparing with the crude extract.”
Claim · model-written summary
Dialysis increased phytase specific activity 10.8-fold over crude extract.
- Taxon
- RICEOryza sativat0
- Treatment
- dialysise0
- Outcome
- specific activity of enzymee0
- Direction
- increasede0
- Value
- 10.8 foldse0
- Comparator
- crude extracte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE BRAN”
e0“The specific activity of enzyme was increased by 7.4 and 10.8 folds after ammonium sulphate fractionation and dialysis, respectively comparing with the crude extract.”
Claim · model-written summary
Partially purified rice-bran phytase had an optimum pH of 5.0.
- Taxon
- RICEOryza sativat0
- Outcome
- optimum pHe0
- Value
- 5.0e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme showed an optimum pH of 5.0 and optimum temperature of 50°C.”
t0“RICE BRAN”
Claim · model-written summary
Partially purified rice-bran phytase had an optimum temperature of 50°C.
- Taxon
- RICEOryza sativat0
- Outcome
- optimum temperaturee0
- Value
- 50°Ce0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme showed an optimum pH of 5.0 and optimum temperature of 50°C.”
t0“RICE BRAN”
Claim · model-written summary
Rice-bran phytase retained about 80% activity after 10 minutes at 60°C.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE BRAN”
e0“the enzyme retained about 80% and 30% of its activity when heated for 10 min. at 60 and 70°C, respectively.”
Claim · model-written summary
Rice-bran phytase retained about 30% activity after 10 minutes at 70°C.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE BRAN”
e0“the enzyme retained about 80% and 30% of its activity when heated for 10 min. at 60 and 70°C, respectively.”
Claim · model-written summary
Enzyme activity declined at high substrate concentrations and was optimal at 3.0 mM sodium phytate.
- Taxon
- RICEOryza sativat0
- Compound
- sodium phytatee0
- Outcome
- enzyme activitye0
- Direction
- reducede0
- Value
- 3.0 mMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE BRAN”
e0“The enzyme activity was reduced at high substrate concentrations with an optimum substrate concentration of 3.0 mM sodium phytate.”
Claim · model-written summary
The phytase Km was 1.66x10-3 M.
- Taxon
- RICEOryza sativat0
- Outcome
- Km valuee0
- Value
- 1.66x10-3 Me0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE BRAN”
e0“The Km value was found to be 1.66x10-3 M and the Vmax was 0.95 µM Pi liberated/min/ml enzyme.”
Claim · model-written summary
The phytase Vmax was 0.95 µM Pi liberated/min/ml enzyme.
- Taxon
- RICEOryza sativat0
- Outcome
- Vmaxe0
- Value
- 0.95 µM Pi liberated/min/ml enzymee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE BRAN”
e0“The Km value was found to be 1.66x10-3 M and the Vmax was 0.95 µM Pi liberated/min/ml enzyme.”
Claim · model-written summary
CoCl2 and MnSo4 activated the phytase reaction at 1x10-4 or 1x10-3 M.
- Taxon
- RICEOryza sativat0
- Treatment
- CoCl2, MnSo4e0
- Outcome
- enzyme reactione0
- Direction
- activatinge0
- Value
- 1x10-4 or 1x10-3 Me0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE BRAN”
e0“Each of CoCl2, MnSo4, CaCl2 and MgSO4 showed an activating effect on the enzyme reaction when added at concentrations of 1x10-4 or 1x10-3 M.”
Claim · model-written summary
CaCl2 and MgSO4 activated the phytase reaction at 1x10-4 or 1x10-3 M.
- Taxon
- RICEOryza sativat0
- Treatment
- CaCl2 and MgSO4e0
- Outcome
- enzyme reactione0
- Direction
- activatinge0
- Value
- 1x10-4 or 1x10-3 Me0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE BRAN”
e0“Each of CoCl2, MnSo4, CaCl2 and MgSO4 showed an activating effect on the enzyme reaction when added at concentrations of 1x10-4 or 1x10-3 M.”
Claim · model-written summary
ZnSO4 and NiCl2 partly inhibited phytase activity.
- Taxon
- RICEOryza sativat0
- Treatment
- ZnSO4 and NiCl2e0
- Outcome
- enzyme activitye0
- Direction
- partial inhibitorye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE BRAN”
e0“both ZnSO4 and NiCl2 caused partial inhibitory effect, while FeSO4 and CuSO4 were found to strongly inhibit the enzyme activity.”
Claim · model-written summary
FeSO4 and CuSO4 strongly inhibited phytase activity.
- Taxon
- RICEOryza sativat0
- Treatment
- FeSO4 and CuSO4e0
- Outcome
- enzyme activitye0
- Direction
- strongly inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE BRAN”
e0“both ZnSO4 and NiCl2 caused partial inhibitory effect, while FeSO4 and CuSO4 were found to strongly inhibit the enzyme activity.”
The same questions in other papers
iron(2+) sulfate (anhydrous) · soil: biology · Oryza sativa (rice)Positive and negative results
1 in the opposite direction
- positive
zinc sulfate · soil: biology · Oryza sativa (rice)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.