Abstract
A chimeric gene consisting of the coding sequence for chloroplastic Fe superoxide dismutase (FeSOD) from Arabidopsis thaliana, coupled to the chloroplast targeting sequence from the pea ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit, was expressed in Nicotiana tabacum cv Petit Havana SR1. Expression of the transgenic FeSOD protected both the plasmalemma and photosystem II against superoxide generated during illumination of leaf discs impregnated with methyl viologen. By contrast, overproduction of a mitochondrial MnSOD from Nicotiana plumbaginifolia in the chloroplasts of cv SR1 protected only the plasmalemma, but not photosystem II, against methyl viologen (L. Slooten, K. Capiau, W. Van Camp, M. Van Montagu, C. Sybesma, D. Inzé [1995] Plant Physiol 107: 737-750). The difference in effectiveness correlates with different membrane affinities of the transgenic FeSOD and MnSOD. Overproduction of FeSOD does not confer tolerance to H2O2, singlet oxygen, chilling-induced photoinhibition in leaf disc assays, or to salt stress at the whole plant level. In nontransgenic plants, salt stress led to a 2- to 3-fold increase in activity, on a protein basis, of FeSOD, cytosolic and chloroplastic Cu/ZnSOD, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase. In FeSOD-overproducing plants under salt stress, the induction of cytosolic and chloroplastic Cu/ZnSOD was suppressed, whereas induction of a water-soluble chloroplastic ascorbate peroxidase isozyme was promoted.
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Claims 12
Claim · model-written summary
Chloroplastic FeSOD protected tobacco plasmalemma and photosystem II from methyl-viologen-generated superoxide.
- Taxon
- tobacco
Nicotiana tabacumt0 - Organ / stage
- leaf discse0
- Treatment
- methyl viologene0
- Gene
- transgenic FeSODe0
- Outcome
- plasmalemma and photosystem IIe0
- Direction
- protectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic tobacco plants”
e0“Expression of the transgenic FeSOD protected both the plasmalemma and photosystem II against superoxide generated during illumination of leaf discs impregnated with methyl viologen.”
Claim · model-written summary
Chloroplastic MnSOD protected the plasmalemma against methyl viologen.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- cv SR1e0
- Treatment
- methyl viologene0
- Gene
- mitochondrial MnSODe0
- Outcome
- plasmalemmae0
- Direction
- protectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic tobacco plants”
e0“overproduction of a mitochondrial MnSOD from Nicotiana plumbaginifolia in the chloroplasts of cv SR1 protected only the plasmalemma, but not photosystem II, against methyl viologen”
Claim · model-written summary
Chloroplastic MnSOD did not protect photosystem II against methyl viologen.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- cv SR1e0
- Treatment
- methyl viologene0
- Gene
- mitochondrial MnSODe0
- Outcome
- photosystem IIe0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic tobacco plants”
e0“overproduction of a mitochondrial MnSOD from Nicotiana plumbaginifolia in the chloroplasts of cv SR1 protected only the plasmalemma, but not photosystem II, against methyl viologen”
Claim · model-written summary
The difference in FeSOD and MnSOD effectiveness correlated with their membrane affinities.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- transgenic FeSOD and MnSODe0
- Outcome
- difference in effectivenesse0
- Qualifier
- different membrane affinitiese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic tobacco plants”
e0“The difference in effectiveness correlates with different membrane affinities of the transgenic FeSOD and MnSOD.”
Claim · model-written summary
FeSOD overproduction did not confer tolerance to H2O2.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- H2O2e0
- Gene
- FeSODe0
- Outcome
- tolerance to H2O2e0
- Direction
- does not confere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic tobacco plants”
e0“Overproduction of FeSOD does not confer tolerance to H2O2, singlet oxygen, chilling-induced photoinhibition in leaf disc assays, or to salt stress at the whole plant level.”
Claim · model-written summary
FeSOD overproduction did not confer tolerance to singlet oxygen.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- singlet oxygene0
- Gene
- FeSODe0
- Outcome
- tolerance to H2O2, singlet oxygene0
- Direction
- does not confere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic tobacco plants”
e0“Overproduction of FeSOD does not confer tolerance to H2O2, singlet oxygen, chilling-induced photoinhibition in leaf disc assays, or to salt stress at the whole plant level.”
Claim · model-written summary
FeSOD overproduction did not confer tolerance to chilling-induced photoinhibition in leaf disc assays.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- leaf disce0
- Treatment
- chilling-induced photoinhibitione0
- Gene
- FeSODe0
- Outcome
- chilling-induced photoinhibitione0
- Direction
- does not confere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic tobacco plants”
e0“Overproduction of FeSOD does not confer tolerance to H2O2, singlet oxygen, chilling-induced photoinhibition in leaf disc assays, or to salt stress at the whole plant level.”
Claim · model-written summary
FeSOD overproduction did not confer salt-stress tolerance at whole-plant level.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- salt stresse0
- Gene
- FeSODe0
- Outcome
- tolerance to H2O2, singlet oxygen, chilling-induced photoinhibition in leaf disc assays, or to salt stresse0
- Direction
- does not confere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic tobacco plants”
e0“Overproduction of FeSOD does not confer tolerance to H2O2, singlet oxygen, chilling-induced photoinhibition in leaf disc assays, or to salt stress at the whole plant level.”
Claim · model-written summary
Salt stress increased FeSOD and cytosolic and chloroplastic Cu/ZnSOD activity two- to threefold in nontransgenic plants.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- salt stresse0
- Outcome
- activity, on a protein basis, of FeSOD, cytosolic and chloroplastic Cu/ZnSODe0
- Direction
- increasee0
- Value
- 2- to 3-folde0
- Comparator
- nontransgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic tobacco plants”
e0“In nontransgenic plants, salt stress led to a 2- to 3-fold increase in activity, on a protein basis, of FeSOD, cytosolic and chloroplastic Cu/ZnSOD, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase.”
Claim · model-written summary
Salt stress increased ascorbate peroxidase, dehydroascorbate reductase and glutathione reductase activity two- to threefold in nontransgenic plants.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- salt stresse0
- Outcome
- ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductasee0
- Direction
- increasee0
- Value
- 2- to 3-folde0
- Comparator
- nontransgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic tobacco plants”
e0“In nontransgenic plants, salt stress led to a 2- to 3-fold increase in activity, on a protein basis, of FeSOD, cytosolic and chloroplastic Cu/ZnSOD, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase.”
Claim · model-written summary
Under salt stress, FeSOD overproduction suppressed induction of cytosolic and chloroplastic Cu/ZnSOD.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- salt stresse0
- Outcome
- induction of cytosolic and chloroplastic Cu/ZnSODe0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic tobacco plants”
e0“In FeSOD-overproducing plants under salt stress, the induction of cytosolic and chloroplastic Cu/ZnSOD was suppressed, whereas induction of a water-soluble chloroplastic ascorbate peroxidase isozyme was promoted.”
Claim · model-written summary
Under salt stress, FeSOD overproduction promoted induction of a water-soluble chloroplastic ascorbate peroxidase isozyme.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- salt stresse0
- Outcome
- induction of a water-soluble chloroplastic ascorbate peroxidase isozymee0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic tobacco plants”
e0“In FeSOD-overproducing plants under salt stress, the induction of cytosolic and chloroplastic Cu/ZnSOD was suppressed, whereas induction of a water-soluble chloroplastic ascorbate peroxidase isozyme was promoted.”
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