Abstract
Food availability represents a major worldwide concern due to climate change and population growth. Low-temperature stress (LTS) severely restricts the growth of tomato seedlings. Exogenous 5-aminolevulinic acid (ALA) can alleviate the harm of abiotic stress including LTS; however, data on its protective mechanism on tomato seedling roots, the effects of organelle structure, and the regulation of metabolic pathways under LTS are lacking. In this study, we hope to fill the above gaps by exploring the effects of exogenous ALA on morphology, mitochondrial ultrastructure, reactive oxygen species (ROS) enrichment, physiological indicators, related gene expression, and metabolic pathway in tomato seedlings root under LTS. Results showed that ALA pretreatment could increase the activity of antioxidant enzymes and the content of antioxidant substances in tomato seedlings roots under LTS to scavenge the massively accumulated ROS, thereby protecting the mitochondrial structure of roots and promoting root development under LTS. Combined transcriptomic and metabolomic analysis showed that exogenous ALA pretreatment activated the glutathione metabolism and β-alanine metabolism of tomato seedling roots under LTS, further enhanced the scavenging ability of tomato seedling roots to ROS, and improved the low-temperature tolerance of tomato seedlings. The findings provide a new insight into the regulation of the low-temperature tolerance of tomato by exogenous ALA.
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Claims 9
Claim · model-written summary
ALA pretreatment increased antioxidant enzyme activity in tomato seedling roots under low-temperature stress.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- rootse0
- Treatment
- ALA pretreatmente0
- Outcome
- activity of antioxidant enzymese0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ALA pretreatment could increase the activity of antioxidant enzymes and the content of antioxidant substances in tomato seedlings roots under LTS”
Claim · model-written summary
ALA pretreatment increased antioxidant substance content in tomato seedling roots under low-temperature stress.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- rootse0
- Treatment
- ALA pretreatmente0
- Outcome
- content of antioxidant substancese0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ALA pretreatment could increase the activity of antioxidant enzymes and the content of antioxidant substances in tomato seedlings roots under LTS”
Claim · model-written summary
ALA pretreatment scavenged accumulated ROS in tomato seedling roots under low-temperature stress.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- rootse0
- Treatment
- Exogenous 5-aminolevulinic acidt0
- Outcome
- massively accumulated ROSe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“to scavenge the massively accumulated ROS, thereby protecting the mitochondrial structure of roots and promoting root development under LTS”
t0“Exogenous 5-aminolevulinic acid alleviates low-temperature injury by regulating glutathione metabolism and β-alanine metabolism in tomato seedling roots”
Claim · model-written summary
ALA pretreatment protected the mitochondrial structure of tomato seedling roots under low-temperature stress.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- protecting the mitochondrial structure of rootse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protecting the mitochondrial structure of roots and promoting root development under LTS”
t0“tomato seedling roots”
Claim · model-written summary
ALA pretreatment promoted tomato root development under low-temperature stress.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- root developmente0
- Direction
- promotinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoting root development under LTS”
t0“tomato seedling roots”
Claim · model-written summary
Exogenous ALA pretreatment activated glutathione metabolism in tomato seedling roots under low-temperature stress.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- exogenous ALA pretreatmente0
- Outcome
- glutathione metabolisme0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous ALA pretreatment activated the glutathione metabolism and β-alanine metabolism of tomato seedling roots under LTS”
Claim · model-written summary
Exogenous ALA pretreatment activated β-alanine metabolism in tomato seedling roots under low-temperature stress.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- exogenous ALA pretreatmente0
- Outcome
- β-alanine metabolisme0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous ALA pretreatment activated the glutathione metabolism and β-alanine metabolism of tomato seedling roots under LTS”
Claim · model-written summary
ALA pretreatment enhanced ROS scavenging in tomato seedling roots.
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- scavenging ability of tomato seedling roots to ROSe0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“further enhanced the scavenging ability of tomato seedling roots to ROS”
Claim · model-written summary
ALA pretreatment improved low-temperature tolerance of tomato seedlings.
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- low-temperature tolerancee0
- Direction
- improvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“improved the low-temperature tolerance of tomato seedlings”
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.