Abstract
The effect of callus source (shoot tip, axillary bud and leaf primordium), medium (growth regulators) and number of subcultures on both somatic embryogenesis and shoot formation were studied. Moreover, the anatomical features of roots and leaves of regenerated plantlets were also studied in comparison with those of seedlings. Results revealed that shoot tip callus on a medium containing 2mg/L IBA gave the best somatic embryogenesis. Hormone free medium and that containing 5 mg/L 2, ip did not cause somaclonal variation, while on other media it ranged between 14.42 and 32.15%. On transferring onto free hormone medium, 20% of the abnormal embryos developed normal plantlets. The highest rate of shoot multiplication was obtained with shoot tip callus on medium containing 3mg/L 2,ip. Comparative anatomy of in vitro and ex vitro leaves and roots, indicated that the leaves of the in vitro regenerated plantlets had well developed tissues. On the contrary, their roots were characterized by poorly developed cortex and vascular bundles, which may be the main causes of the observed problems with plantlet acclimatization. Abbreviations. 2,4-D: 2,4 dichlorophenoxyacetic acid; NAA: naphthaleneacetic acid; IBA: indole-3- butyric acid; 2, ip: (2-dimethylallayl – amino)- purin; GA3: gibberellic acid.
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Claims 9
Claim · model-written summary
Shoot tip callus on 2mg/L IBA medium gave the best somatic embryogenesis.
- Taxon
- DATE PALM
Phoenix dactyliferat0 - Treatment
- shoot tip callus on a medium containing 2mg/L IBAe0
- Outcome
- somatic embryogenesise0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“DATE PALM (Phoenix dactylifera L.)”
e0“shoot tip callus on a medium containing 2mg/L IBA gave the best somatic embryogenesis”
Claim · model-written summary
Hormone free medium did not cause somaclonal variation.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Treatment
- Hormone free mediume0
- Outcome
- somaclonal variatione0
- Direction
- did not causee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“DATE PALM (Phoenix dactylifera L.)”
e0“Hormone free medium and that containing 5 mg/L 2, ip did not cause somaclonal variation”
Claim · model-written summary
Medium containing 5 mg/L 2, ip did not cause somaclonal variation.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Treatment
- 5 mg/L 2, ipe0
- Outcome
- somaclonal variatione0
- Direction
- did not causee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“DATE PALM (Phoenix dactylifera L.)”
e0“Hormone free medium and that containing 5 mg/L 2, ip did not cause somaclonal variation”
Claim · model-written summary
Other media had somaclonal variation of 14.42 to 32.15%.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Treatment
- other mediae0
- Outcome
- it rangede0
- Value
- 14.42 and 32.15%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“DATE PALM (Phoenix dactylifera L.)”
e0“while on other media it ranged between 14.42 and 32.15%”
Claim · model-written summary
On transfer to hormone free medium, 20% of abnormal embryos developed normal plantlets.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Treatment
- free hormone mediume0
- Outcome
- abnormal embryos developed normal plantletse0
- Value
- 20%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“DATE PALM (Phoenix dactylifera L.)”
e0“On transferring onto free hormone medium, 20% of the abnormal embryos developed normal plantlets”
Claim · model-written summary
Shoot tip callus on 3mg/L 2,ip medium yielded the highest shoot multiplication rate.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Treatment
- shoot tip callus on medium containing 3mg/L 2,ipe0
- Outcome
- rate of shoot multiplicatione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“DATE PALM (Phoenix dactylifera L.)”
e0“The highest rate of shoot multiplication was obtained with shoot tip callus on medium containing 3mg/L 2,ip”
Claim · model-written summary
In vitro regenerated leaves had well developed tissues.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Organ / stage
- leavese0
- Outcome
- well developed tissuese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“DATE PALM (Phoenix dactylifera L.)”
e0“the leaves of the in vitro regenerated plantlets had well developed tissues”
Claim · model-written summary
In vitro regenerated roots had poorly developed cortex and vascular bundles.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Organ / stage
- rootse0
- Outcome
- poorly developed cortex and vascular bundlese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“DATE PALM (Phoenix dactylifera L.)”
e0“their roots were characterized by poorly developed cortex and vascular bundles”
Claim · model-written summary
Poorly developed root structures may explain plantlet acclimatization problems.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Organ / stage
- rootse0
- Outcome
- problems with plantlet acclimatizatione0
- Qualifier
- poorly developed cortex and vascular bundlese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“DATE PALM (Phoenix dactylifera L.)”
e0“their roots were characterized by poorly developed cortex and vascular bundles, which may be the main causes of the observed problems with plantlet acclimatization”
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See all 51 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.