Abstract
The trans-Golgi network (TGN) acts as a central platform for sorting and secreting various cargoes to the cell surface, thus being essential for the full execution of plant immunity. However, the fine-tuned regulation of TGN components in plant defense and stress response has been not fully elucidated. Our study revealed that despite largely compromising penetration resistance, the loss-of-function mutation of the TGN component protein ECHIDNA (ECH) induced enhanced postinvasion resistance to powdery mildew in Arabidopsis thaliana. Genetic and transcriptome analyses and hormone profiling demonstrated that ECH loss resulted in salicylic acid (SA) hyperaccumulation via the ISOCHORISMATE SYNTHASE 1 biosynthesis pathway, thereby constitutively activating SA-dependent innate immunity that was largely responsible for the enhanced postinvasion resistance. Furthermore, the ech mutant displayed accelerated SA-independent spontaneous cell death and constitutive POWDERY MILDEW RESISTANCE 4-mediated callose depositions. In addition, ECH loss led to a chronically prolonged endoplasmic reticulum stress in the ech mutant. These results provide insights into understanding the role of TGN components in the regulation of plant immunity and stress responses.
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Claims 7
Claim · model-written summary
ECH loss compromised penetration resistance to powdery mildew.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- loss-of-function mutatione0
- Interactor
- powdery mildewe0pathogen
- Gene
- ECHIDNA (ECH)e0
- Outcome
- penetration resistancee0
- Direction
- compromisinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“despite largely compromising penetration resistance, the loss-of-function mutation of the TGN component protein ECHIDNA (ECH) induced enhanced postinvasion resistance to powdery mildew in Arabidopsis thaliana”
Claim · model-written summary
ECH loss enhanced postinvasion resistance to powdery mildew.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- loss-of-function mutatione0
- Interactor
- powdery mildewe0pathogen
- Gene
- ECHIDNA (ECH)e0
- Outcome
- postinvasion resistance to powdery mildewe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the loss-of-function mutation of the TGN component protein ECHIDNA (ECH) induced enhanced postinvasion resistance to powdery mildew in Arabidopsis thaliana”
Claim · model-written summary
ECH loss caused salicylic acid hyperaccumulation via ISOCHORISMATE SYNTHASE 1 biosynthesis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- ISOCHORISMATE SYNTHASE 1e0
- Compound
- salicylic acid (SA)e0
- Outcome
- salicylic acid (SA) hyperaccumulatione0
- Direction
- hyperaccumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ECH loss resulted in salicylic acid (SA) hyperaccumulation via the ISOCHORISMATE SYNTHASE 1 biosynthesis pathway”
e1“in Arabidopsis thaliana”
Claim · model-written summary
SA hyperaccumulation constitutively activated SA-dependent innate immunity, largely accounting for enhanced postinvasion resistance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Compound
- SAe1
- Outcome
- SA-dependent innate immunitye0
- Direction
- activatinge0
- Qualifier
- largely responsible for the enhanced postinvasion resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“thereby constitutively activating SA-dependent innate immunity that was largely responsible for the enhanced postinvasion resistance”
e1“salicylic acid (SA) hyperaccumulation”
e2“in Arabidopsis thaliana”
Claim · model-written summary
The ech mutant had accelerated spontaneous cell death independent of SA.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- ech mutante0
- Outcome
- SA-independent spontaneous cell deathe0
- Direction
- acceleratede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ech mutant displayed accelerated SA-independent spontaneous cell death”
e1“in Arabidopsis thaliana”
Claim · model-written summary
The ech mutant had constitutive callose deposition mediated by POWDERY MILDEW RESISTANCE 4.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- ech mutante0
- Gene
- POWDERY MILDEW RESISTANCE 4e0
- Outcome
- callose depositionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ech mutant displayed accelerated SA-independent spontaneous cell death and constitutive POWDERY MILDEW RESISTANCE 4-mediated callose depositions”
e1“in Arabidopsis thaliana”
Claim · model-written summary
ECH loss prolonged endoplasmic reticulum stress chronically.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- ECH losse0
- Gene
- ECHe0
- Outcome
- endoplasmic reticulum stresse0
- Direction
- prolongede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ECH loss led to a chronically prolonged endoplasmic reticulum stress in the ech mutant”
e1“in Arabidopsis thaliana”
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