Abstract
Ascorbic acid (AsA) is a multifunctional phytonutrient that is essential for the human diet as well as plant development. While much is known about AsA biosynthesis in plants, how this process is regulated in tomato (Solanum lycopersicum) fruits remains unclear. Here, we found that auxin treatment inhibited AsA accumulation in the leaves and pericarps of tomato. The auxin response factor gene SlARF4 is induced by auxin to mediate auxin-induced inhibition of AsA accumulation. Specifically, SlARF4 transcriptionally inhibits the transcription factor gene SlMYB11, thereby modulating AsA accumulation by regulating the transcription of the AsA biosynthesis genes l-galactose-1-phosphate phosphatase, l-galactono-1,4-lactone dehydrogenase, and dehydroascorbate. By contrast, abscisic acid (ABA) treatment increased AsA accumulation in tomato under drought stress. ABA induced the expression of the mitogen-activated protein kinase gene SlMAPK8. We demonstrate that SlMAPK8 phosphorylates SlARF4 and inhibits its transcriptional activity, whereas SlMAPK8 phosphorylates SlMYB11 and activates its transcriptional activity. SlMAPK8 functions in ABA-induced AsA accumulation and drought stress tolerance. Moreover, ABA antagonizes the effects of auxin on AsA biosynthesis. Therefore, auxin- and ABA-induced regulation of AsA accumulation is mediated by the SlMAPK8-SlARF4-SlMYB11 module in tomato during fruit development and drought stress responses, shedding light on the roles of phytohormones in regulating AsA accumulation to mediate stress tolerance.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
Auxin treatment inhibited ascorbic acid accumulation in tomato leaves and pericarps.
- Taxon
- tomato
Solanum lycopersicume1 - Organ / stage
- leaves and pericarpse1
- Treatment
- auxin treatmente1
- Outcome
- AsA accumulatione1
- Direction
- inhibitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“auxin treatment inhibited AsA accumulation in the leaves and pericarps of tomato”
Claim · model-written summary
Auxin induced the auxin response factor gene SlARF4 in tomato.
- Taxon
- tomatoSolanum lycopersicume2
- Treatment
- auxine1
- Gene
- SlARF4e1
- Outcome
- auxin response factor gene SlARF4e1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The auxin response factor gene SlARF4 is induced by auxin”
e2“tomato (Solanum lycopersicum)”
Claim · model-written summary
SlARF4 mediates auxin-induced inhibition of ascorbic acid accumulation in tomato.
- Taxon
- tomatoSolanum lycopersicume3
- Treatment
- auxine1
- Gene
- SlARF4e2
- Outcome
- AsA accumulatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“to mediate auxin-induced inhibition of AsA accumulation”
e2“The auxin response factor gene SlARF4”
e3“tomato (Solanum lycopersicum)”
Claim · model-written summary
SlARF4 transcriptionally inhibits the transcription of the transcription factor gene SlMYB11 in tomato.
- Taxon
- tomatoSolanum lycopersicume2
- Gene
- SlMYB11e1
- Outcome
- transcriptione1
- Qualifier
- transcriptionally inhibitse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“SlARF4 transcriptionally inhibits the transcription factor gene SlMYB11”
e2“tomato (Solanum lycopersicum)”
Claim · model-written summary
SlARF4 modulates ascorbic acid accumulation by regulating the transcription of three AsA biosynthesis genes in tomato.
- Taxon
- tomatoSolanum lycopersicume2
- Gene
- l-galactose-1-phosphate phosphatase, l-galactono-1,4-lactone dehydrogenase, and dehydroascorbatee1
- Outcome
- AsA accumulatione1
- Qualifier
- by regulating the transcriptione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“thereby modulating AsA accumulation by regulating the transcription of the AsA biosynthesis genes l-galactose-1-phosphate phosphatase, l-galactono-1,4-lactone dehydrogenase, and dehydroascorbate”
e2“tomato (Solanum lycopersicum)”
Claim · model-written summary
Abscisic acid treatment increased ascorbic acid accumulation in tomato under drought stress.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- abscisic acid (ABA) treatmente1
- Outcome
- AsA accumulatione1
- Direction
- increasede1
- Qualifier
- under drought stresse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“abscisic acid (ABA) treatment increased AsA accumulation in tomato under drought stress”
Claim · model-written summary
ABA induced the expression of the mitogen-activated protein kinase gene SlMAPK8 in tomato.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“ABA induced the expression of the mitogen-activated protein kinase gene SlMAPK8”
e2“tomato (Solanum lycopersicum)”
Claim · model-written summary
SlMAPK8 phosphorylates SlARF4 and inhibits its transcriptional activity.
- Taxon
- tomatoSolanum lycopersicume2
- Gene
- SlMAPK8e1
- Outcome
- transcriptional activitye1
- Qualifier
- phosphorylates SlARF4e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“SlMAPK8 phosphorylates SlARF4 and inhibits its transcriptional activity”
e2“tomato (Solanum lycopersicum)”
Claim · model-written summary
SlMAPK8 phosphorylates SlMYB11 and activates its transcriptional activity.
- Taxon
- tomatoSolanum lycopersicume2
- Gene
- SlMAPK8e1
- Outcome
- transcriptional activitye1
- Qualifier
- phosphorylates SlMYB11e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“SlMAPK8 phosphorylates SlMYB11 and activates its transcriptional activity”
e2“tomato (Solanum lycopersicum)”
Claim · model-written summary
SlMAPK8 functions in ABA-induced ascorbic acid accumulation and drought stress tolerance in tomato.
- Taxon
- tomatoSolanum lycopersicume2
- Gene
- SlMAPK8e1
- Outcome
- AsA accumulation and drought stress tolerancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“SlMAPK8 functions in ABA-induced AsA accumulation and drought stress tolerance”
e2“tomato (Solanum lycopersicum)”
Claim · model-written summary
ABA antagonizes the effects of auxin on ascorbic acid biosynthesis in tomato.
- Taxon
- tomatoSolanum lycopersicume2
- Treatment
- ABAe1
- Outcome
- effects of auxin on AsA biosynthesise1
- Direction
- antagonizese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“ABA antagonizes the effects of auxin on AsA biosynthesis”
e2“tomato (Solanum lycopersicum)”
Claim · model-written summary
Auxin- and ABA-induced regulation of ascorbic acid accumulation is mediated by the SlMAPK8-SlARF4-SlMYB11 module in tomato during fruit development and drought stress responses.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- SlMAPK8-SlARF4-SlMYB11e1
- Outcome
- AsA accumulatione1
- Qualifier
- during fruit development and drought stress responsese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“auxin- and ABA-induced regulation of AsA accumulation is mediated by the SlMAPK8-SlARF4-SlMYB11 module in tomato during fruit development and drought stress responses”
The same questions in other papers
(+)-abscisic acid · molecular: metabolites · Solanum lycopersicum (tomato)Positive and negative results
9 papers in the same direction as this paper · 5 in the opposite direction
- negative
- no effect
- no effect
11 papers more in this group
See all 15 papers →class · molecular: metabolites · Solanum lycopersicum (tomato)Positive and negative results
6 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.