Abstract
KEY MESSAGE: Our results show that SPL12 plays a crucial role in regulating nodule development in Medicago sativa L. (alfalfa), and that AGL6 is targeted and downregulated by SPL12. Root architecture in plants is critical because of its role in controlling nutrient cycling, water use efficiency and response to biotic and abiotic stress factors. The small RNA, microRNA156 (miR156), is highly conserved in plants, where it functions by silencing a group of SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors. We previously showed that transgenic Medicago sativa (alfalfa) plants overexpressing miR156 display increased nodulation, improved nitrogen fixation and enhanced root regenerative capacity during vegetative propagation. In alfalfa, transcripts of eleven SPLs, including SPL12, are targeted for cleavage by miR156. In this study, we characterized the role of SPL12 in root architecture and nodulation by investigating the transcriptomic and phenotypic changes associated with altered transcript levels of SPL12, and by determining SPL12 regulatory targets using SPL12-silencing and -overexpressing alfalfa plants. Phenotypic analyses showed that silencing of SPL12 in alfalfa caused an increase in root regeneration, nodulation, and nitrogen fixation. In addition, AGL6 which encodes AGAMOUS-like MADS box transcription factor, was identified as being directly targeted for silencing by SPL12, based on Next Generation Sequencing-mediated transcriptome analysis and chromatin immunoprecipitation assays. Taken together, our results suggest that SPL12 and AGL6 form a genetic module that regulates root development and nodulation in alfalfa.
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Claims 5
Claim · model-written summary
SPL12 silencing increased alfalfa root regeneration.
- Taxon
- alfalfa
Medicago sativae0 - Treatment
- silencing of SPL12e0
- Gene
- SPL12e0
- Outcome
- root regeneratione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of SPL12 in alfalfa caused an increase in root regeneration, nodulation, and nitrogen fixation.”
Claim · model-written summary
SPL12 silencing increased alfalfa nodulation.
- Taxon
- alfalfaMedicago sativae0
- Treatment
- silencing of SPL12e0
- Gene
- SPL12e0
- Outcome
- nodulatione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of SPL12 in alfalfa caused an increase in root regeneration, nodulation, and nitrogen fixation.”
Claim · model-written summary
SPL12 silencing increased alfalfa nitrogen fixation.
- Taxon
- alfalfaMedicago sativae0
- Treatment
- silencing of SPL12e0
- Gene
- SPL12e0
- Outcome
- nitrogen fixatione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of SPL12 in alfalfa caused an increase in root regeneration, nodulation, and nitrogen fixation.”
Claim · model-written summary
SPL12 directly targeted AGL6 for silencing.
- Taxon
- Medicago sativaMedicago sativat0
- Gene
- AGL6e0
- Outcome
- directly targeted for silencing by SPL12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AGL6 which encodes AGAMOUS-like MADS box transcription factor, was identified as being directly targeted for silencing by SPL12”
t0“Medicago sativa”
Claim · model-written summary
The authors propose an SPL12–AGL6 module regulating root development and nodulation.
- Taxon
- alfalfaMedicago sativae0
- Gene
- SPL12 and AGL6e0
- Outcome
- root development and nodulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SPL12 and AGL6 form a genetic module that regulates root development and nodulation in alfalfa.”
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