Abstract
Abiotic stresses cause oxidative damage in plants. Here, we demonstrate that foliar application of an extract from the seaweed Ascophyllum nodosum, SuperFifty (SF), largely prevents paraquat (PQ)-induced oxidative stress in Arabidopsis thaliana. While PQ-stressed plants develop necrotic lesions, plants pre-treated with SF (i.e., primed plants) were unaffected by PQ. Transcriptome analysis revealed induction of reactive oxygen species (ROS) marker genes, genes involved in ROS-induced programmed cell death, and autophagy-related genes after PQ treatment. These changes did not occur in PQ-stressed plants primed with SF. In contrast, upregulation of several carbohydrate metabolism genes, growth, and hormone signaling as well as antioxidant-related genes were specific to SF-primed plants. Metabolomic analyses revealed accumulation of the stress-protective metabolite maltose and the tricarboxylic acid cycle intermediates fumarate and malate in SF-primed plants. Lipidome analysis indicated that those lipids associated with oxidative stress-induced cell death and chloroplast degradation, such as triacylglycerols (TAGs), declined upon SF priming. Our study demonstrated that SF confers tolerance to PQ-induced oxidative stress in A. thaliana, an effect achieved by modulating a range of processes at the transcriptomic, metabolic, and lipid levels.
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Claims 7
Claim · model-written summary
Foliar application of Ascophyllum nodosum extract SuperFifty largely prevented paraquat-induced oxidative stress in Arabidopsis thaliana.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- foliar application of an extract from the seaweed Ascophyllum nodosum, SuperFifty (SF)e1
- Outcome
- paraquat (PQ)-induced oxidative stresse1
- Direction
- preventse1
- Comparator
- PQ-stressed plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e1“foliar application of an extract from the seaweed Ascophyllum nodosum, SuperFifty (SF), largely prevents paraquat (PQ)-induced oxidative stress in Arabidopsis thaliana”
e2“While PQ-stressed plants develop necrotic lesions, plants pre-treated with SF (i.e., primed plants) were unaffected by PQ”
Claim · model-written summary
Arabidopsis plants primed with SF were unaffected by paraquat, whereas PQ-stressed plants developed necrotic lesions.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- plants pre-treated with SFe2
- Outcome
- necrotic lesionse2
- Direction
- unaffectede2
- Comparator
- PQ-stressed plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e2“While PQ-stressed plants develop necrotic lesions, plants pre-treated with SF (i.e., primed plants) were unaffected by PQ”
Claim · model-written summary
Paraquat treatment induced reactive oxygen species marker genes, programmed cell death genes and autophagy-related genes in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- PQ treatmente3
- Outcome
- reactive oxygen species (ROS) marker genes, genes involved in ROS-induced programmed cell death, and autophagy-related genese3
- Direction
- inductione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e3“Transcriptome analysis revealed induction of reactive oxygen species (ROS) marker genes, genes involved in ROS-induced programmed cell death, and autophagy-related genes after PQ treatment”
Claim · model-written summary
The paraquat-induced transcriptional changes did not occur in plants primed with SF compared with PQ-stressed plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- primed with SFe4
- Outcome
- changese4
- Direction
- did not occure4
- Comparator
- PQ-stressed plantse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e4“These changes did not occur in PQ-stressed plants primed with SF”
Claim · model-written summary
SF-primed plants accumulated the stress-protective metabolite maltose and the TCA cycle intermediates fumarate and malate.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- SF-primed plantse5
- Outcome
- accumulation of the stress-protective metabolite maltose and the tricarboxylic acid cycle intermediates fumarate and malatee5
- Direction
- accumulatione5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e5“Metabolomic analyses revealed accumulation of the stress-protective metabolite maltose and the tricarboxylic acid cycle intermediates fumarate and malate in SF-primed plants”
Claim · model-written summary
Triacylglycerols declined in SF-primed plants under paraquat stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- SF priminge6
- Outcome
- triacylglycerols (TAGs)e6
- Direction
- declinede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e6“such as triacylglycerols (TAGs), declined upon SF priming”
Claim · model-written summary
Upregulation of carbohydrate metabolism, growth and hormone signaling genes and of antioxidant-related genes was specific to SF-primed plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- SF-primed plantse7
- Outcome
- upregulation of several carbohydrate metabolism genes, growth, and hormone signaling as well as antioxidant-related genese7
- Direction
- upregulatione7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e7“upregulation of several carbohydrate metabolism genes, growth, and hormone signaling as well as antioxidant-related genes were specific to SF-primed plants”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.