Paper · 2022 · The Pakistan Journal of Agricultural Sciences

Potentiality of native Trichoderma harzianum in controlling damping off and foot rot of chilli and its viability in different storage conditions

M. M. Islam

13
claims
25
verbatim quotes
2
citations
2
research areas
—
Not open access

Abstract

The potentiality of native Trichoderma harzianum against damping off and foot rot of chilli caused by Sclerotium rolfsii and Fusarium oxysporum, respectively as well as its shelf life as formulated product in different storage conditions was evaluated. In dual culture, native T. harzianum inhibited the mycelial growth of S. rolfsii by 60.56% and F. oxysporum by 81.67% over control. In green house conditions, soil received both formulated T. harzianum (20 g/kg of soil) and T. harzianum suspension (2.45×107 CFU/mL) resulted reduced severity of damping off by 81.80% and foot rot by 84.61% over control; however, the combined application of both the treatments was also resulted the highest seed germination (70.28%). The formulated T. harzianum (@ 50 g/polyethylene bags) was stored in table top, wooden shelve, tin container, wooden locker at room temperature, and in refrigerator at 4 °C. The spore density was constantly increased from the initial amount (22×106 CFU/g) in all the treatments and reached the peak after 45 days in wooden locker (73×106 CFU/g) and tin container (66×106 CFU/g) where, it reached the peak at 60 days in table top (126.3×106 CFU/g), wooden shelve (109×106 CFU/g) and refrigerator (89.67×106 CFU/g). However, the maximum population density (126.3×106 CFU/g) was observed in table top storage at 60 days which also remained highest even after 120 days (51.67×106 CFU/g) in comparison to other treatments. The findings of the study revealed that the native T. harzianum can be exploited as an effective bio-control agent for the successful disease control program in chilli as it showed extended shelf life in common storage facility.

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Claims 13

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In dual culture, native T. harzianum inhibited mycelial growth of S. rolfsii by 60.56% over control.

Taxon
chillit0
Treatment
native T. harzianume0
Interactor
S. rolfsiiAgroathelia rolfsiie0pathogen
Second interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
mycelial growthe0
Direction
inhibitede0
Value
60.56%e0
Comparator
controle0
Setting
dual culturee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Agroathelia rolfsii
Agroathelia rolfsii · InteractorEdward L. Barnard - Florida Department of Agriculture and Consumer Services · CC BY 3.0 us · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

t0

“chilli”

✓ located verbatim in the source · title, characters 88–94 · show in text ↑
e0

“In dual culture, native T. harzianum inhibited the mycelial growth of S. rolfsii by 60.56% and F. oxysporum by 81.67% over control”

✓ located verbatim in the source · abstract, characters 253–383 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In dual culture, native T. harzianum inhibited mycelial growth of F. oxysporum by 81.67% over control.

Taxon
chillit0
Treatment
native T. harzianume0
Interactor
F. oxysporumFusarium oxysporume0pathogen
Second interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
mycelial growthe0
Direction
inhibitede0
Value
81.67%e0
Comparator
controle0
Setting
dual culturee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

t0

“chilli”

✓ located verbatim in the source · title, characters 88–94 · show in text ↑
e0

“In dual culture, native T. harzianum inhibited the mycelial growth of S. rolfsii by 60.56% and F. oxysporum by 81.67% over control”

✓ located verbatim in the source · abstract, characters 253–383 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In the greenhouse, soil receiving formulated T. harzianum and T. harzianum suspension reduced the severity of damping off by 81.80% over control.

Taxon
chillit0
Treatment
formulated T. harzianum (20 g/kg of soil) and T. harzianum suspension (2.45×107 CFU/mL)e0
Interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
severity of damping offe0
Direction
reducede0
Value
81.80%e0
Comparator
controle0
Setting
green house conditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

t0

“chilli”

✓ located verbatim in the source · title, characters 88–94 · show in text ↑
e0

“In green house conditions, soil received both formulated T. harzianum (20 g/kg of soil) and T. harzianum suspension (2.45×107 CFU/mL) resulted reduced severity of damping off by 81.80% and foot rot by 84.61% over control”

✓ located verbatim in the source · abstract, characters 385–605 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In the greenhouse, soil receiving formulated T. harzianum and T. harzianum suspension reduced the severity of foot rot by 84.61% over control.

Taxon
chillit0
Treatment
formulated T. harzianum (20 g/kg of soil) and T. harzianum suspension (2.45×107 CFU/mL)e0
Interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
foot rote0
Direction
reducede0
Value
84.61%e0
Comparator
controle0
Setting
green house conditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

t0

“chilli”

✓ located verbatim in the source · title, characters 88–94 · show in text ↑
e0

“In green house conditions, soil received both formulated T. harzianum (20 g/kg of soil) and T. harzianum suspension (2.45×107 CFU/mL) resulted reduced severity of damping off by 81.80% and foot rot by 84.61% over control”

✓ located verbatim in the source · abstract, characters 385–605 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The combined application of both treatments gave the highest seed germination (70.28%).

Taxon
chillit0
Treatment
combined application of both the treatmentse0
Outcome
seed germinatione0
Direction
higheste0
Value
70.28%e0

Not reportedComparator

Verbatim

t0

“chilli”

✓ located verbatim in the source · title, characters 88–94 · show in text ↑
e0

“the combined application of both the treatments was also resulted the highest seed germination (70.28%)”

✓ located verbatim in the source · abstract, characters 616–719 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The spore density of the formulated T. harzianum increased constantly from the initial 22x10^6 CFU/g in all storage treatments.

Taxon
Trichoderma harzianumTrichoderma harzianumt1
Outcome
spore densitye0
Direction
increasede0
Value
22×106 CFU/ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma harzianum
Trichoderma harzianum · TaxonUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

t1

“Trichoderma harzianum”

✓ located verbatim in the source · title, characters 23–44 · show in text ↑
e0

“The spore density was constantly increased from the initial amount (22×106 CFU/g) in all the treatments”

✓ located verbatim in the source · abstract, characters 895–998 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Spore density peaked after 45 days in the wooden locker at 73x10^6 CFU/g.

Taxon
Trichoderma harzianumTrichoderma harzianumt1
Outcome
peake0
Value
73×106 CFU/ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma harzianum
Trichoderma harzianum · TaxonUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

t1

“Trichoderma harzianum”

✓ located verbatim in the source · title, characters 23–44 · show in text ↑
e0

“reached the peak after 45 days in wooden locker (73×106 CFU/g) and tin container (66×106 CFU/g)”

✓ located verbatim in the source · abstract, characters 1003–1098 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Spore density peaked after 45 days in the tin container at 66x10^6 CFU/g.

Taxon
Trichoderma harzianumTrichoderma harzianumt1
Outcome
peake0
Value
66×106 CFU/ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma harzianum
Trichoderma harzianum · TaxonUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

t1

“Trichoderma harzianum”

✓ located verbatim in the source · title, characters 23–44 · show in text ↑
e0

“reached the peak after 45 days in wooden locker (73×106 CFU/g) and tin container (66×106 CFU/g)”

✓ located verbatim in the source · abstract, characters 1003–1098 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Spore density peaked at 60 days in table top storage at 126.3x10^6 CFU/g.

Taxon
Trichoderma harzianumTrichoderma harzianumt1
Outcome
peake0
Value
126.3×106 CFU/ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma harzianum
Trichoderma harzianum · TaxonUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

t1

“Trichoderma harzianum”

✓ located verbatim in the source · title, characters 23–44 · show in text ↑
e0

“it reached the peak at 60 days in table top (126.3×106 CFU/g), wooden shelve (109×106 CFU/g) and refrigerator (89.67×106 CFU/g)”

✓ located verbatim in the source · abstract, characters 1106–1233 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Spore density peaked at 60 days in the wooden shelf at 109x10^6 CFU/g.

Taxon
Trichoderma harzianumTrichoderma harzianumt1
Outcome
peake0
Value
109×106 CFU/ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma harzianum
Trichoderma harzianum · TaxonUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

t1

“Trichoderma harzianum”

✓ located verbatim in the source · title, characters 23–44 · show in text ↑
e0

“it reached the peak at 60 days in table top (126.3×106 CFU/g), wooden shelve (109×106 CFU/g) and refrigerator (89.67×106 CFU/g)”

✓ located verbatim in the source · abstract, characters 1106–1233 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Spore density peaked at 60 days in the refrigerator at 89.67x10^6 CFU/g.

Taxon
Trichoderma harzianumTrichoderma harzianumt1
Outcome
peake0
Value
89.67×106 CFU/ge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma harzianum
Trichoderma harzianum · TaxonUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

t1

“Trichoderma harzianum”

✓ located verbatim in the source · title, characters 23–44 · show in text ↑
e0

“it reached the peak at 60 days in table top (126.3×106 CFU/g), wooden shelve (109×106 CFU/g) and refrigerator (89.67×106 CFU/g)”

✓ located verbatim in the source · abstract, characters 1106–1233 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The maximum population density (126.3x10^6 CFU/g) was in table top storage at 60 days and remained highest after 120 days (51.67x10^6 CFU/g) compared with other treatments.

Taxon
Trichoderma harzianumTrichoderma harzianumt1
Outcome
maximum population densitye0
Value
126.3×106 CFU/ge0
Comparator
other treatmentse0
Setting
table top storagee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trichoderma harzianum
Trichoderma harzianum · TaxonUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

t1

“Trichoderma harzianum”

✓ located verbatim in the source · title, characters 23–44 · show in text ↑
e0

“the maximum population density (126.3×106 CFU/g) was observed in table top storage at 60 days which also remained highest even after 120 days (51.67×106 CFU/g) in comparison to other treatments”

✓ located verbatim in the source · abstract, characters 1244–1437 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors conclude that native T. harzianum can be exploited as an effective bio-control agent for disease control in chilli.

Taxon
chillie0
Outcome
effective bio-control agente0

Verbatim

e0

“the native T. harzianum can be exploited as an effective bio-control agent for the successful disease control program in chilli”

✓ located verbatim in the source · abstract, characters 1479–1606 · show in text ↑

Trichoderma harzianum · Agroathelia rolfsii · Capsicum (peppers)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Trichoderma harzianum · Fusarium oxysporum · Capsicum (peppers)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Trichoderma harzianum · disease · Capsicum (peppers)Results agree

16 papers: 16 positive, 0 negative or no effect

15 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

12 papers more in this group

See all 16 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.