Abstract
When being stressed, plants require a balance between the resistance pathway and metabolism. Glucosinolates (GS) are secondary metabolics that widely exist in Brassicaceae. Glutathione (GSH) not only participates in plant processing reactive oxygen species (ROS) but also directly participates in GS synthesis as a sulfur donor. Therefore, we used transcriptomic to identify antioxidant and GS metabolism responses in GSH-treated pakchoi. Our study elucidated that GSH can be used as priming to improve oxidative resistance and preferentially stimulate the expression of resistance genes such as CAT1. The reduction in transcription factor expression inhibits the key steps of the GS synthesis pathway. When ROS returned to normal level, the resistance gene decreased and returned to normal level, while GSH restored the gene expression of GS biosynthesis. This work puts forward the mechanism of GSH in regulating the antioxidant system and glucosinolate metabolic pathway, which provides a basis for further study on the relationship between environmental signals and plant metabolism and provides ideas for follow-up research.
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Claims 5
Claim · model-written summary
GSH priming improved oxidative resistance in pakchoi.
Not reportedComparator
Verbatim
e0“GSH-treated pakchoi”
e1“GSH can be used as priming to improve oxidative resistance and preferentially stimulate the expression of resistance genes such as CAT1”
Claim · model-written summary
GSH priming preferentially stimulated expression of resistance genes such as CAT1 in pakchoi.
Not reportedComparator
Verbatim
e0“GSH-treated pakchoi”
e1“GSH can be used as priming to improve oxidative resistance and preferentially stimulate the expression of resistance genes such as CAT1”
Claim · model-written summary
A reduction in transcription factor expression inhibits the key steps of the glucosinolate synthesis pathway in GSH-treated pakchoi.
Verbatim
e0“GSH-treated pakchoi”
e1“The reduction in transcription factor expression inhibits the key steps of the GS synthesis pathway”
Claim · model-written summary
When ROS returned to normal level, the resistance gene expression decreased and returned to normal level.
Verbatim
e0“GSH-treated pakchoi”
e1“When ROS returned to normal level, the resistance gene decreased and returned to normal level”
Claim · model-written summary
GSH restored the gene expression of glucosinolate biosynthesis.
Not reportedComparator
Verbatim
e0“GSH-treated pakchoi”
e1“GSH restored the gene expression of GS biosynthesis”
The same questions in other papers
glutathione · molecular: expression · Brassica rapa (field mustard)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.