Abstract
The present study on the identification of volatiles and secondary metabolites for endophytic Bacillus subtilis was carried out for the management of soil borne diseases of cotton using native isolates of endophytic Bacillus sp. A total of 20 endophytic bacteria were isolated from different cotton plant origin. Biochemical characterization of bacterial endophytes revealed that all the 20 isolates were found to be Bacillus sp. It was also confirmed through Polymerase Chain Reaction (PCR) by amplifying an amplicon of 520 bp of 16s rDNA of Bacillus sp. Among the twenty isolates of Bacillus sp. tested for their antagonistic activity against Rhizoctonia bataticola by dual culture, ESBs 19 significantly exerted the highest of 57.91% reduction of mycelial growth. Application of endophytic Bacillus subtilis (5 g kg-1) as seed treatment reduced the incidence of wilt disease by 65.57% under field conditions against the control. In the present study the MIDI Sherlock System identified the bacterial antagonist as Bacillus subtilis by fatty profiles, with a degree of similarity index (SI) of 0.822, with 0.12 separation between the first and second choice.
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Claims 6
Claim · model-written summary
Twenty endophytic bacteria were isolated from cotton plants.
- Taxon
- cotton plant
Gossypium hirsutume0 - Interactor
- endophytic bacteriae0symbiont
- Outcome
- endophytic bacteria were isolatede0
- Value
- 20e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 20 endophytic bacteria were isolated from different cotton plant origin.”
Claim · model-written summary
Biochemical characterization assigned all 20 cotton endophytes to Bacillus sp.
- Taxon
- cottonGossypium hirsutumt0
- Interactor
- Bacillus sp.e0symbiont
- Outcome
- all the 20 isolates were found to be Bacillus sp.e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Biochemical characterization of bacterial endophytes revealed that all the 20 isolates were found to be Bacillus sp.”
t0“cotton”
Claim · model-written summary
PCR confirmed Bacillus sp. identity with a 520 bp 16s rDNA amplicon.
- Taxon
- cottonGossypium hirsutumt0
- Interactor
- Bacillus sp.e0symbiont
- Outcome
- confirmed through Polymerase Chain Reaction (PCR)e0
- Value
- 520 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was also confirmed through Polymerase Chain Reaction (PCR) by amplifying an amplicon of 520 bp of 16s rDNA of Bacillus sp.”
t0“cotton”
Claim · model-written summary
ESBs 19 reduced Rhizoctonia bataticola mycelial growth by 57.91% in dual culture.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- ESBs 19e0
- Interactor
- Rhizoctonia bataticola
Macrophomina phaseolinae0pathogen - Second interactor
- Bacillus sp.e0biocontrol_agent
- Outcome
- Rhizoctonia bataticola by dual culturee0
- Direction
- reductione0
- Value
- 57.91%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the twenty isolates of Bacillus sp. tested for their antagonistic activity against Rhizoctonia bataticola by dual culture, ESBs 19 significantly exerted the highest of 57.91% reduction of mycelial growth.”
t0“cotton”
Claim · model-written summary
Bacillus subtilis seed treatment reduced cotton wilt incidence by 65.57% versus control in the field.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- endophytic Bacillus subtilis (5 g kg-1) as seed treatmente0
- Interactor
- Rhizoctonia bataticolaMacrophomina phaseolinat0pathogen
- Second interactor
- Bacillus subtilis
Bacillus subtilise0biocontrol_agent - Outcome
- incidence of wilt diseasee0
- Direction
- reducede0
- Value
- 65.57%e0
- Comparator
- the controle0
- Setting
- field conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of endophytic Bacillus subtilis (5 g kg-1) as seed treatment reduced the incidence of wilt disease by 65.57% under field conditions against the control.”
t0“Rhizoctonia bataticola causing root rot in cotton”
Claim · model-written summary
Fatty acid profiling identified the antagonist as Bacillus subtilis with similarity index 0.822.
- Taxon
- cottonGossypium hirsutumt0
- Interactor
- Bacillus subtilisBacillus subtilise0biocontrol_agent
- Outcome
- identified the bacterial antagonist as Bacillus subtilise0
- Value
- 0.822e0
- Qualifier
- 0.12 separation between the first and second choicee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the MIDI Sherlock System identified the bacterial antagonist as Bacillus subtilis by fatty profiles, with a degree of similarity index (SI) of 0.822, with 0.12 separation between the first and second choice.”
t0“cotton”
The same questions in other papers
seed treatment · Macrophomina phaseolina · Gossypium hirsutum (cotton)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.