Abstract
To investigate a possible involvement of nitric oxide in gene regulation of glutathione and flavonoid synthesis pathways, maize seedlings were treated with sodium nitroprusside (SNP), a NO donor, and apocynin (APO), an inducer of NO production. After 12-h treatment, the transcripts of γ-glutamylcysteine synthetase (γ-ecs), glutathione synthetase (gsh-s), chalcone synthase (chs), phenylalanine ammonia lyase (pal.1), myb-related protein P (P1) and actin 1 (act) genes were quantified in maize leaves by real time PCR, using α-tubulin as standard transcript. The level of γ-ecs and gsh-s transcripts in maize leaves were increased 9-fold and 12-fold, respectively, following SNP treatment, while after APO treatment, those transcripts were not significantly different from control plants. SNP-treated maize leaves did not show significant changes in pal.1 and chs expression. NO content in maize leaves was increased in SNP and APO treated plants in comparison to control plants. In conclusion, our experiments suggested that genes involved in glutathione synthesis could be modulated by SNP in maize leaves. On the other hand, APO had no effect on γ-ecs and gshs gene expression.
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Claims 8
Claim · model-written summary
SNP increased γ-ecs transcripts ninefold.
- Treatment
- SNP treatmente0
- Gene
- γ-ecse0
- Outcome
- level of γ-ecs and gsh-s transcriptse0
- Direction
- increasede0
- Value
- 9-folde0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of γ-ecs and gsh-s transcripts in maize leaves were increased 9-fold and 12-fold, respectively, following SNP treatment”
Claim · model-written summary
SNP increased gsh-s transcripts twelvefold.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of γ-ecs and gsh-s transcripts in maize leaves were increased 9-fold and 12-fold, respectively, following SNP treatment”
Claim · model-written summary
APO did not significantly change γ-ecs transcripts from controls.
- Treatment
- APO treatmente0
- Outcome
- transcriptse0
- Direction
- not significantly differente0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after APO treatment, those transcripts were not significantly different from control plants”
t0“Zea mays leaves”
Claim · model-written summary
APO did not significantly change gsh-s transcripts from controls.
- Treatment
- APO treatmente0
- Outcome
- transcriptse0
- Direction
- not significantly differente0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after APO treatment, those transcripts were not significantly different from control plants”
t0“Zea mays leaves”
Claim · model-written summary
SNP did not significantly change pal.1 expression.
- Treatment
- SNP-treatede0
- Gene
- pal.1e0
- Outcome
- pal.1 and chs expressione0
- Direction
- did not show significant changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SNP-treated maize leaves did not show significant changes in pal.1 and chs expression”
Claim · model-written summary
SNP did not significantly change chs expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SNP-treated maize leaves did not show significant changes in pal.1 and chs expression”
Claim · model-written summary
SNP increased maize leaf NO content against controls.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO content in maize leaves was increased in SNP and APO treated plants in comparison to control plants”
Claim · model-written summary
APO increased maize leaf NO content against controls.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO content in maize leaves was increased in SNP and APO treated plants in comparison to control plants”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.