Abstract
Influences of in vitro salinity in form of sodium chloride (NaCl) and calcium chloride (CaCl2) on growth and element contents of three grape cultivars (Thompson Seedless, Red Roomy and Beauty Seedless) were investigated. Shoot tip explants were cultured on Murashige and Skoog (MS) medium containing 1.0 mg/l benzylaminopurine (BAP). The experiment was conducted with four levels of NaCl (0, 50, 85 and 120 mM) and four levels of CaCl2 (0, 1, 5 and 10 mM) and mixture of both salts with three levels [0, 60 (50 NaCl+10 CaCl2) and 90 (85 NaCl+5 CaCl2) mM]. The results showed that the growth parameters (proliferation, plantlet height, number of leaves, number of nodes, internode length, fresh and dry weight) significantly decreased with increasing NaCl concentrations up to 120 mM compared to the control treatment. However, application of CaCl2 treatments counteracted this inhibitory effect on the growth parameters at 5 mM. CaCl2 supply increased shoot Ca2+ content and decreased shoot Na+ content. Increasing NaCl and CaCl2 concentrations in the culture medium increased the contents of Na+, Ca2+, whereas K+ content decreased compared to the control.
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Claims 7
Claim · model-written summary
Increasing NaCl to 120 mM significantly decreased grape growth traits versus control.
Verbatim
e0“the growth parameters (proliferation, plantlet height, number of leaves, number of nodes, internode length, fresh and dry weight) significantly decreased with increasing NaCl concentrations up to 120 mM compared to the control treatment”
t0“Three Grape”
Claim · model-written summary
CaCl2 at 5 mM counteracted inhibition of grape growth.
Not reportedComparator
Verbatim
e0“application of CaCl2 treatments counteracted this inhibitory effect on the growth parameters at 5 mM”
t0“Three Grape”
Claim · model-written summary
CaCl2 supply increased grape shoot Ca2+ content.
Not reportedComparator
Verbatim
e0“CaCl2 supply increased shoot Ca2+ content”
t0“Three Grape”
Claim · model-written summary
CaCl2 supply decreased grape shoot Na+ content.
Not reportedComparator
Verbatim
e0“CaCl2 supply increased shoot Ca2+ content and decreased shoot Na+ content”
t0“Three Grape”
Claim · model-written summary
Increasing medium NaCl and CaCl2 raised grape Na+ content.
- Taxon
- grapet0
- Treatment
- Increasing NaCl and CaCl2 concentrations in the culture mediume0
- Outcome
- contents of Na+e0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“Increasing NaCl and CaCl2 concentrations in the culture medium increased the contents of Na+, Ca2+”
t0“Three Grape”
Claim · model-written summary
Increasing medium NaCl and CaCl2 raised grape Ca2+ content.
- Taxon
- grapet0
- Treatment
- Increasing NaCl and CaCl2 concentrations in the culture mediume0
- Outcome
- Ca2+e0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“Increasing NaCl and CaCl2 concentrations in the culture medium increased the contents of Na+, Ca2+”
t0“Three Grape”
Claim · model-written summary
Increasing medium NaCl and CaCl2 reduced grape K+ content versus control.
Verbatim
e0“K+ content decreased compared to the control”
t0“Three Grape”
e1“Increasing NaCl and CaCl2 concentrations in the culture medium”
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.