Abstract
SummaryThe main bottleneck of mutation induction in vegetatively propagated ornamentals is the development of chimera. Ephemeral small sectors apparent on petals cannot generally be recovered as solid mutant shoots. An efficient technique has been standardized in the present experiment for direct shoot regeneration from individual florets of...chrysanthemum (Chrysanthemum morifolium Ramat.) cv. Colchi Bahar. The efficiency of direct shoot regeneration was tested on 16 media based on MS basal medium containing sucrose, agar and different concentrations and combinations of BAP and NAA. The regenerative potential varied with the developmental stages of the flower head. The highest percentage of shoot formation and most shoots per responding explant were observed on florets taken from stage II flower heads. Direct shoot formation was confirmed by scanning electron microscopy and histological observations. The technique has been tested on 16 other cultivars in the medium containing 0.2 mg l21 NAA and 1.mg.l1 BAP. Gamma ray of 1.5 and 2.0 krad induced yellow and white floret colour sectors in `Maghi' (original colour-mauve) have been established in pure form using this technique. The regenerated plants were rooted in vitro and successfully transferred to soil where they grew vigorously and flowered true to the floret colour type.
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Claims 7
Claim · model-written summary
The highest percentage of shoot formation and most shoots per responding explant were observed on florets from stage II flower heads.
- Taxon
- chrysanthemum
.jpeg?width=60)
Chrysanthemumt0 - Organ / stage
- florets taken from stage II flower headse1
- Outcome
- percentage of shoot formatione1
- Direction
- higheste1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpeg?width=500)
Verbatim
t0“Management of chimera through direct shoot regeneration from florets of chrysanthemum (Chrysanthemum morifolium Ramat.)”
e1“The highest percentage of shoot formation and most shoots per responding explant were observed on florets taken from stage II flower heads”
Claim · model-written summary
The regenerative potential varied with the developmental stages of the flower head.
- Taxon
- chrysanthemumChrysanthemumt0
- Organ / stage
- developmental stages of the flower heade2
- Outcome
- regenerative potentiale2
- Direction
- variede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Management of chimera through direct shoot regeneration from florets of chrysanthemum (Chrysanthemum morifolium Ramat.)”
e2“The regenerative potential varied with the developmental stages of the flower head”
Claim · model-written summary
Direct shoot formation was confirmed by scanning electron microscopy and histological observations.
- Taxon
- chrysanthemumChrysanthemumt0
- Outcome
- Direct shoot formatione3
- Qualifier
- scanning electron microscopy and histological observationse3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Management of chimera through direct shoot regeneration from florets of chrysanthemum (Chrysanthemum morifolium Ramat.)”
e3“Direct shoot formation was confirmed by scanning electron microscopy and histological observations”
Claim · model-written summary
Gamma ray of 1.5 and 2.0 krad induced yellow and white floret colour sectors in Maghi, which were established in pure form by direct shoot regeneration.
- Taxon
- chrysanthemumChrysanthemumt0
- Material
- Maghie4
- Treatment
- Gamma ray of 1.5 and 2.0 krade4
- Outcome
- yellow and white floret colour sectorse4
- Comparator
- original colour-mauvee4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Management of chimera through direct shoot regeneration from florets of chrysanthemum (Chrysanthemum morifolium Ramat.)”
e4“Gamma ray of 1.5 and 2.0 krad induced yellow and white floret colour sectors in `Maghi' (original colour-mauve) have been established in pure form using this technique”
Claim · model-written summary
An efficient direct shoot regeneration technique from individual florets was standardized, tested on 16 media.
- Taxon
- chrysanthemumChrysanthemumt0
- Outcome
- efficient techniquee5
- Comparator
- 16 mediae6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Management of chimera through direct shoot regeneration from florets of chrysanthemum (Chrysanthemum morifolium Ramat.)”
e5“An efficient technique has been standardized in the present experiment for direct shoot regeneration from individual florets”
e6“The efficiency of direct shoot regeneration was tested on 16 media”
Claim · model-written summary
Regenerated plants were rooted in vitro and successfully transferred to soil.
- Taxon
- chrysanthemumChrysanthemumt0
- Outcome
- rooted in vitro and successfully transferred to soile7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Management of chimera through direct shoot regeneration from florets of chrysanthemum (Chrysanthemum morifolium Ramat.)”
e7“The regenerated plants were rooted in vitro and successfully transferred to soil where they grew vigorously and flowered true to the floret colour type”
Claim · model-written summary
Regenerated plants grew vigorously and flowered true to the floret colour type.
- Taxon
- chrysanthemumChrysanthemumt0
- Outcome
- flowered true to the floret colour typee7
- Qualifier
- grew vigorouslye7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Management of chimera through direct shoot regeneration from florets of chrysanthemum (Chrysanthemum morifolium Ramat.)”
e7“The regenerated plants were rooted in vitro and successfully transferred to soil where they grew vigorously and flowered true to the floret colour type”
The same questions in other papers
radiation · quality: postharvest · ChrysanthemumResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.