Abstract
Anthocyanins are secondary metabolites of plants. They have a wide range of biological activity such as antioxidant, photoprotection, osmoregulation, heavy metal ions chelation, antimicrobial and antifungal activities, which help plants to survive under different stress conditions. Bread wheat (T. aestivum L.) can have purple pigmentation provided by anthocyanin compounds in different organs, such as grain pericarp, coleoptile, culm, leaf blades, leaf sheaths, glumes and anthers. However, the genetic mechanisms underlying formation of these traits as well as contribution of the pigmentation to stress tolerance have not been widely studied in wheat. The aim of the current study was to investigate molecular-genetic mechanisms underlying anthocyanin pigmentation in different wheat organs and to estimate the role of the pigmentation under different abiotic stress conditions in wheat seedlings. In the current study, near-isogenic lines (NILs): cv. ‘Saratovskaya 29’ (‘S29’) and lines i:S29Pp1Pp2PF and i:S29Pp1Pp3P developed on the ‘S29’ background but having grain pericarp coloration (genes Pp) and more intense coleoptile (Rc), culm (Pc), leaf blade (Plb), leaf sheath (Pls) pigmentation in comparison with ‘S29’, were used. Comparative transcriptional analysis of the five structural genes Chs, Chi, F3h, Dfr, Ans, encoding enzymes participating in the anthocyanin biosynthesis, was performed in different organs of NILs. It was shown that the presence of the Rc, Pc, Plb, Pls and Pp alleles conferring strong anthocyanin pigmentation induced more intense transcription of the structural genes, suggesting the genes Rc, Pc, Plb, Pls and Pp to play a regulatory role in anthocyanin biosynthesis network. To evaluate the role of anthocyanins in stress response at the seedling stage, growth ability of the NILs and anthocyanin content in their coleoptiles were assessed after treatments with NaCl (100 and 200 mM), CdCl2 (25 and 50 μM) and 15% PEG 6000 (polyethylene glycol 6000), simulating salinity, heavy metal and drought stress, respectively. Under salinity and drought stress, the level of anthocyanins increased significantly in all three NILs in comparison with untreated control, whereas under CdCl2 treatment anthocyanin content increased significantly in ‘S29’ only. The tendency of the lines having more intensive anthocyanin pigmentation to have better growth ability under stress conditions was observed. Taken together the results obtained it may be suggested that anthocyanin production in wheat seedlings is tightly related with the response to abiotic stress.
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Claims 7
Claim · model-written summary
Pigmentation alleles increased transcription of five structural anthocyanin genes.
- Taxon
- Bread wheat
Triticum aestivume1 - Gene
- Rc, Pc, Plb, Pls and Ppe0
- Outcome
- transcription of the structural genese0
- Direction
- more intensee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the presence of the Rc, Pc, Plb, Pls and Pp alleles conferring strong anthocyanin pigmentation induced more intense transcription of the structural genes”
e1“Bread wheat (T. aestivum L.) can have purple pigmentation”
Claim · model-written summary
Authors suggest the pigmentation alleles regulate anthocyanin biosynthesis.
Verbatim
e0“suggesting the genes Rc, Pc, Plb, Pls and Pp to play a regulatory role in anthocyanin biosynthesis network”
t0“TRITICUM AESTIVUM L.”
Claim · model-written summary
Salinity significantly increased anthocyanins in all three lines.
Verbatim
e0“Under salinity and drought stress, the level of anthocyanins increased significantly in all three NILs in comparison with untreated control”
t0“TRITICUM AESTIVUM L.”
Claim · model-written summary
Drought significantly increased anthocyanins in all three lines.
Verbatim
e0“Under salinity and drought stress, the level of anthocyanins increased significantly in all three NILs in comparison with untreated control”
t0“TRITICUM AESTIVUM L.”
Claim · model-written summary
Cadmium chloride significantly increased anthocyanins only in S29.
- Taxon
- TRITICUM AESTIVUM L.t0
- Material
- ‘S29’e0
- Treatment
- CdCl2 treatmente0
- Outcome
- anthocyanin contente0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“under CdCl2 treatment anthocyanin content increased significantly in ‘S29’ only”
t0“TRITICUM AESTIVUM L.”
Claim · model-written summary
More pigmented lines tended to grow better under stress.
Verbatim
e0“The tendency of the lines having more intensive anthocyanin pigmentation to have better growth ability under stress conditions was observed.”
t0“TRITICUM AESTIVUM L.”
Claim · model-written summary
Authors suggest anthocyanin production is tied to abiotic stress response.
Not reportedDirection
Verbatim
e0“anthocyanin production in wheat seedlings is tightly related with the response to abiotic stress”
t0“TRITICUM AESTIVUM L.”
The same questions in other papers
cadmium dichloride · quality: phytochemicals · Triticum aestivum (wheat)Positive and negative results
1 in the opposite direction
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salinity · quality: phytochemicals · Triticum aestivum (wheat)Results differ
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- no effect
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See all 15 papers →drought · quality: phytochemicals · Triticum aestivum (wheat)Results differ
12 papers in the same direction as this paper · 12 in the opposite direction
- negative
- negative
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See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.