Paper · 2021 · Applied Sciences

Effect of Vacuum Impregnation with Sucrose and Plant Growth Hormones to Mitigate the Chilling Injury in Spinach Leaves

Nazatul Umira Karim; Noor Liyana Yusof

11
claims
22
verbatim quotes
7
citations
2
research areas
✓
Open access · cc-by

Abstract

Vacuum impregnation (VI) has been immensely used in modifying the physicochemical properties, nutritional values and sensory attributes of fruits and vegetables. However, the metabolic consequences of the plant tissue upon impregnation have not been profoundly explored although shelf life is strongly dependent on this factor. In this study, spinach leaves were impregnated with salicylic acid (SA), γ-aminobutyric acid (GABA) and sucrose to improve its quality and storage ability by reducing the chilling injury through the improvement of proline content. The spinach leaves were stored at 4 °C for 7 days and were analyzed at 12 h interval. Upon 1 day of impregnation, the proline content in GABA, sucrose and SA impregnated leaves was increased by 240%, 153% and 103%, respectively, while in non-impregnated leaves, the proline content was decreased by 23.8%. The chlorophyll content of GABA impregnated leaves exhibited the lowest reduction (49%) followed by sucrose (55%) and SA (57%); meanwhile, non-impregnated leaves reduced 80% of chlorophyll content at the end of storage. Sensory evaluation showed that GABA, sucrose and SA impregnated leaves respectively, obtained higher score in terms of freshness, color, texture and overall appearance as compared to non-impregnated leaves.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 11

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

GABA impregnation increased spinach leaf proline content by 240% after one day.

Treatment
GABAe0
Outcome
proline contente0
Direction
increasede0
Value
240%e0
Qualifier
1 daye0
Comparator
non-impregnated leavese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Upon 1 day of impregnation, the proline content in GABA, sucrose and SA impregnated leaves was increased by 240%, 153% and 103%, respectively, while in non-impregnated leaves, the proline content was decreased by 23.8%.”

✓ located verbatim in the source · abstract, characters 645–864 · show in text ↑
e1

“In this study, spinach leaves were impregnated with salicylic acid (SA), γ-aminobutyric acid (GABA) and sucrose”

✓ located verbatim in the source · abstract, characters 328–439 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sucrose impregnation increased spinach leaf proline content by 153% after one day.

Treatment
sucrosee0
Outcome
proline contente0
Direction
increasede0
Value
153%e0
Comparator
non-impregnated leavese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Upon 1 day of impregnation, the proline content in GABA, sucrose and SA impregnated leaves was increased by 240%, 153% and 103%, respectively, while in non-impregnated leaves, the proline content was decreased by 23.8%.”

✓ located verbatim in the source · abstract, characters 645–864 · show in text ↑
e1

“In this study, spinach leaves were impregnated with salicylic acid (SA), γ-aminobutyric acid (GABA) and sucrose”

✓ located verbatim in the source · abstract, characters 328–439 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

SA impregnation increased spinach leaf proline content by 103% after one day.

Treatment
SAe0
Outcome
proline contente0
Direction
increasede0
Value
103%e0
Comparator
non-impregnated leavese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Upon 1 day of impregnation, the proline content in GABA, sucrose and SA impregnated leaves was increased by 240%, 153% and 103%, respectively, while in non-impregnated leaves, the proline content was decreased by 23.8%.”

✓ located verbatim in the source · abstract, characters 645–864 · show in text ↑
e1

“In this study, spinach leaves were impregnated with salicylic acid (SA), γ-aminobutyric acid (GABA) and sucrose”

✓ located verbatim in the source · abstract, characters 328–439 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Proline content decreased 23.8% in non-impregnated spinach leaves.

Treatment
non-impregnatede0
Outcome
proline contente0
Direction
decreasede0
Value
23.8%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“in non-impregnated leaves, the proline content was decreased by 23.8%”

✓ located verbatim in the source · abstract, characters 794–863 · show in text ↑
e1

“In this study, spinach leaves were impregnated with salicylic acid (SA), γ-aminobutyric acid (GABA) and sucrose”

✓ located verbatim in the source · abstract, characters 328–439 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

GABA-impregnated spinach leaves lost 49% of chlorophyll by the end of storage.

Treatment
GABAe0
Outcome
chlorophyll contente0
Direction
reductione0
Value
49%e0
Comparator
non-impregnated leavese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The chlorophyll content of GABA impregnated leaves exhibited the lowest reduction (49%) followed by sucrose (55%) and SA (57%); meanwhile, non-impregnated leaves reduced 80% of chlorophyll content at the end of storage.”

✓ located verbatim in the source · abstract, characters 865–1084 · show in text ↑
e1

“In this study, spinach leaves were impregnated with salicylic acid (SA), γ-aminobutyric acid (GABA) and sucrose”

✓ located verbatim in the source · abstract, characters 328–439 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sucrose-impregnated spinach leaves lost 55% of chlorophyll by the end of storage.

Treatment
sucrosee0
Outcome
chlorophyll contente0
Direction
reductione0
Value
55%e0
Comparator
non-impregnated leavese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The chlorophyll content of GABA impregnated leaves exhibited the lowest reduction (49%) followed by sucrose (55%) and SA (57%); meanwhile, non-impregnated leaves reduced 80% of chlorophyll content at the end of storage.”

✓ located verbatim in the source · abstract, characters 865–1084 · show in text ↑
e1

“In this study, spinach leaves were impregnated with salicylic acid (SA), γ-aminobutyric acid (GABA) and sucrose”

✓ located verbatim in the source · abstract, characters 328–439 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

SA-impregnated spinach leaves lost 57% of chlorophyll by the end of storage.

Treatment
SAe0
Outcome
chlorophyll contente0
Direction
reductione0
Value
57%e0
Comparator
non-impregnated leavese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The chlorophyll content of GABA impregnated leaves exhibited the lowest reduction (49%) followed by sucrose (55%) and SA (57%); meanwhile, non-impregnated leaves reduced 80% of chlorophyll content at the end of storage.”

✓ located verbatim in the source · abstract, characters 865–1084 · show in text ↑
e1

“In this study, spinach leaves were impregnated with salicylic acid (SA), γ-aminobutyric acid (GABA) and sucrose”

✓ located verbatim in the source · abstract, characters 328–439 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Non-impregnated spinach leaves lost 80% of chlorophyll by the end of storage.

Outcome
chlorophyll contente0
Direction
reducede0
Value
80%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“non-impregnated leaves reduced 80% of chlorophyll content at the end of storage”

✓ located verbatim in the source · abstract, characters 1004–1083 · show in text ↑
e1

“In this study, spinach leaves were impregnated with salicylic acid (SA), γ-aminobutyric acid (GABA) and sucrose”

✓ located verbatim in the source · abstract, characters 328–439 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

GABA impregnation improved spinach leaf sensory freshness, color, texture and appearance relative to non-impregnated leaves.

Treatment
GABAe0
Outcome
freshness, color, texture and overall appearancee0
Direction
highere0
Comparator
non-impregnated leavese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“GABA, sucrose and SA impregnated leaves respectively, obtained higher score in terms of freshness, color, texture and overall appearance as compared to non-impregnated leaves.”

✓ located verbatim in the source · abstract, characters 1116–1291 · show in text ↑
e1

“In this study, spinach leaves were impregnated with salicylic acid (SA), γ-aminobutyric acid (GABA) and sucrose”

✓ located verbatim in the source · abstract, characters 328–439 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sucrose impregnation improved spinach leaf sensory freshness, color, texture and appearance.

Treatment
sucrosee0
Outcome
freshness, color, texture and overall appearancee0
Direction
highere0
Comparator
non-impregnated leavese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“GABA, sucrose and SA impregnated leaves respectively, obtained higher score in terms of freshness, color, texture and overall appearance as compared to non-impregnated leaves.”

✓ located verbatim in the source · abstract, characters 1116–1291 · show in text ↑
e1

“In this study, spinach leaves were impregnated with salicylic acid (SA), γ-aminobutyric acid (GABA) and sucrose”

✓ located verbatim in the source · abstract, characters 328–439 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

SA impregnation improved spinach leaf sensory freshness, color, texture and appearance.

Treatment
SAe0
Outcome
freshness, color, texture and overall appearancee0
Direction
highere0
Comparator
non-impregnated leavese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“GABA, sucrose and SA impregnated leaves respectively, obtained higher score in terms of freshness, color, texture and overall appearance as compared to non-impregnated leaves.”

✓ located verbatim in the source · abstract, characters 1116–1291 · show in text ↑
e1

“In this study, spinach leaves were impregnated with salicylic acid (SA), γ-aminobutyric acid (GABA) and sucrose”

✓ located verbatim in the source · abstract, characters 328–439 · show in text ↑

gamma-aminobutyric acid · quality: postharvest · Spinacia oleracea (spinach)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

sucrose · physiology: pigments · Spinacia oleracea (spinach)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.