Abstract
Roses are among the most widely produced cut flowers, with important ornamental and economic value in the flower market. In order to investigate how to regulate flowering periods, the negative regulator of GA biosynthesis RcSPY was cloned from Rosa chinensis and ectopically expressed in tobacco. The overexpression of RcSPY in SPY4 transgenic tobacco significantly increased plant height. Antisense interference with the conserved SPY domain of RcSPY led to GA accumulation in SPY5 transgenic tobacco plants, and antisense interference with the conserved TPR repeats of RcSPY resulted in late flowering in SPY6 transgenic tobacco plants. This result suggests that RcSPY is a negative regulator of GA biosynthesis, since the knock-down of RcSPY increased GA content. However, other phenotypes displayed by transgenic tobacco plants may not be caused by changes of GA content, but rather be the direct effects of RcSPY. Therefore, RcSPY not only participates in GA biosynthesis, but also plays a role in other vegetative and reproductive plant behaviors not related to GA. This result provides theoretical support for the molecular breeding of new cultivars of rose plants.
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Claims 5
Claim · model-written summary
RcSPY overexpression significantly increased tobacco plant height.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- SPY4e0
- Treatment
- overexpression of RcSPYe0
- Gene
- RcSPYe0
- Outcome
- plant heighte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of RcSPY in SPY4 transgenic tobacco significantly increased plant height.”
Claim · model-written summary
Antisense interference with the conserved SPY domain led to GA accumulation in SPY5 tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- SPY5e0
- Treatment
- Antisense interference with the conserved SPY domain of RcSPYe0
- Gene
- RcSPYe0
- Outcome
- GA accumulatione0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense interference with the conserved SPY domain of RcSPY led to GA accumulation in SPY5 transgenic tobacco plants,”
Claim · model-written summary
Antisense interference with conserved TPR repeats caused late flowering in SPY6 tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- SPY6e0
- Treatment
- antisense interference with the conserved TPR repeats of RcSPYe0
- Gene
- RcSPYe0
- Outcome
- late floweringe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“antisense interference with the conserved TPR repeats of RcSPY resulted in late flowering in SPY6 transgenic tobacco plants.”
Claim · model-written summary
The authors interpret RcSPY as a negative regulator of GA biosynthesis.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- RcSPYe0
- Outcome
- GA biosynthesise0
- Direction
- negative regulatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RcSPY is a negative regulator of GA biosynthesis, since the knock-down of RcSPY increased GA content.”
e1“The overexpression of RcSPY in SPY4 transgenic tobacco significantly increased plant height.”
Claim · model-written summary
The authors suggest RcSPY also affects vegetative and reproductive behavior independently of GA.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- RcSPYe0
- Outcome
- other vegetative and reproductive plant behaviorse0
- Qualifier
- not related to GAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RcSPY not only participates in GA biosynthesis, but also plays a role in other vegetative and reproductive plant behaviors not related to GA.”
e1“displayed by transgenic tobacco plants”
The same questions in other papers
loss of function · phenology · Nicotiana tabacum (tobacco)Positive and negative results
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.