Abstract
Salinity stress reduces crop growth and agricultural productions around the world. The effects of salinity stress on seed germination, some morphological, physiological and biochemistry traits, and expression of four genes at seedling stage in salt contrasting barley genotypes were investigated in the current study. Seed germination percentage and morphological traits were analyzed based on a factorial randomized complete design which ANOVA results showed that interaction between salinity (150 and 250 mM NaCl) and genotype (Afzal and Sahand (salt tolerant), and Fajr and Reyhan (sensitive)) was significat (p < 0.05). Also, physiological and biochemical traits were analyzed based on a factorial split plot based on randomized complete design which ANOVA results illustared that interaction between salinity, genotype and time (0, 12, 24 and 72 h after salt treatment) was significat (p < 0.05). Salinity stress significantly declined germination percentage, length and fresh weight of root and shoot, chlorophyll content while thiobarbituric acid reactive material (TBARM), catalase activity, and proline values were increased in root and shoot of all genotypes at seedling stage. The aforementioned traits were more changed with increasing the salt concentration and the higher value of these traits except TBARM were observed in tolerant genotypes. Moreover, relative expression of four genes were investigated after 0, 12, 24 and 72 h of 150 and 250 mM NaCl treatments. The results revealed that expression of HvNHX3, HvCAT1, HvTIP2;3 and Hv3ARK were affected by salt stress and these genes play role in barley tolerance to mild and severe salinity stress based on their expression pattern.
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Claims 9
Claim · model-written summary
Salinity stress significantly declined germination percentage in barley
- Taxon
- barley
Hordeum vulgaret0 - Treatment
- Salinity stresse1
- Outcome
- germination percentagee1
- Direction
- declinede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“Salinity stress significantly declined germination percentage, length and fresh weight of root and shoot, chlorophyll content”
t0“four contrasting barley genotypes under salinity stress”
Claim · model-written summary
Salinity stress significantly declined root and shoot length and fresh weight of barley seedlings
- Taxon
- barley
Hordeum vulgaret0 - Treatment
- Salinity stresse1
- Outcome
- length and fresh weight of root and shoote1
- Direction
- declinede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“Salinity stress significantly declined germination percentage, length and fresh weight of root and shoot, chlorophyll content”
t0“four contrasting barley genotypes under salinity stress”
Claim · model-written summary
Salinity stress significantly declined chlorophyll content in barley seedlings
- Taxon
- barley
Hordeum vulgaret0 - Treatment
- Salinity stresse1
- Outcome
- chlorophyll contente1
- Direction
- declinede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“Salinity stress significantly declined germination percentage, length and fresh weight of root and shoot, chlorophyll content”
t0“four contrasting barley genotypes under salinity stress”
Claim · model-written summary
Salinity stress increased thiobarbituric acid reactive material, catalase activity and proline in barley roots and shoots
- Taxon
- barley
Hordeum vulgaret0 - Treatment
- Salinity stresse1
- Outcome
- thiobarbituric acid reactive material (TBARM), catalase activity, and proline valuese2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“Salinity stress significantly declined germination percentage, length and fresh weight of root and shoot, chlorophyll content”
e2“thiobarbituric acid reactive material (TBARM), catalase activity, and proline values were increased in root and shoot of all genotypes at seedling stage”
t0“four contrasting barley genotypes under salinity stress”
Claim · model-written summary
Higher values of the traits, except TBARM, were observed in salt-tolerant barley genotypes
- Taxon
- barley
Hordeum vulgaret0 - Material
- tolerant genotypese3
- Outcome
- these traitse3
- Direction
- highere3
- Qualifier
- except TBARMe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e3“the higher value of these traits except TBARM were observed in tolerant genotypes”
t0“four contrasting barley genotypes under salinity stress”
Claim · model-written summary
The salinity-induced changes in the traits became larger with increasing salt concentration
- Taxon
- barley
Hordeum vulgaret0 - Treatment
- increasing the salt concentratione4
- Outcome
- aforementioned traitse4
- Direction
- more changede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e4“The aforementioned traits were more changed with increasing the salt concentration”
t0“four contrasting barley genotypes under salinity stress”
Claim · model-written summary
Salt stress affected relative expression of HvNHX3, HvCAT1 and HvTIP2;3 in barley seedlings
- Taxon
- barley
Hordeum vulgaret0 - Treatment
- salt stresse5
- Gene
- HvNHX3, HvCAT1, HvTIP2;3e5
- Outcome
- expressione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e5“expression of HvNHX3, HvCAT1, HvTIP2;3 and Hv3ARK were affected by salt stress”
t0“four contrasting barley genotypes under salinity stress”
Claim · model-written summary
Salt stress affected relative expression of Hv3ARK in barley seedlings
- Taxon
- barley
Hordeum vulgaret0 - Treatment
- salt stresse5
- Gene
- Hv3ARKe5
- Outcome
- expressione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e5“expression of HvNHX3, HvCAT1, HvTIP2;3 and Hv3ARK were affected by salt stress”
t0“four contrasting barley genotypes under salinity stress”
Claim · model-written summary
HvNHX3, HvCAT1, HvTIP2;3 and Hv3ARK play a role in barley tolerance to mild and severe salinity stress
- Taxon
- barley
Hordeum vulgaret0 - Outcome
- tolerance to mild and severe salinity stresse6
- Qualifier
- based on their expression patterne6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e6“these genes play role in barley tolerance to mild and severe salinity stress based on their expression pattern”
t0“four contrasting barley genotypes under salinity stress”
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