Paper · 2021 · Journal of the Saudi Society of Agricultural Sciences

Monitoring of morphological, biochemical and molecular responses of four contrasting barley genotypes under salinity stress

Zeynab Hamzeh-Kahnoji; Asa Ebrahimi; Gholam Reza Sharifi-Sirchi; et al.

9
claims
19
verbatim quotes
6
citations
2
research areas
✓
Open access · cc-by-nc-nd

Abstract

Salinity stress reduces crop growth and agricultural productions around the world. The effects of salinity stress on seed germination, some morphological, physiological and biochemistry traits, and expression of four genes at seedling stage in salt contrasting barley genotypes were investigated in the current study. Seed germination percentage and morphological traits were analyzed based on a factorial randomized complete design which ANOVA results showed that interaction between salinity (150 and 250 mM NaCl) and genotype (Afzal and Sahand (salt tolerant), and Fajr and Reyhan (sensitive)) was significat (p < 0.05). Also, physiological and biochemical traits were analyzed based on a factorial split plot based on randomized complete design which ANOVA results illustared that interaction between salinity, genotype and time (0, 12, 24 and 72 h after salt treatment) was significat (p < 0.05). Salinity stress significantly declined germination percentage, length and fresh weight of root and shoot, chlorophyll content while thiobarbituric acid reactive material (TBARM), catalase activity, and proline values were increased in root and shoot of all genotypes at seedling stage. The aforementioned traits were more changed with increasing the salt concentration and the higher value of these traits except TBARM were observed in tolerant genotypes. Moreover, relative expression of four genes were investigated after 0, 12, 24 and 72 h of 150 and 250 mM NaCl treatments. The results revealed that expression of HvNHX3, HvCAT1, HvTIP2;3 and Hv3ARK were affected by salt stress and these genes play role in barley tolerance to mild and severe salinity stress based on their expression pattern.

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Claims 9

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Salinity stress significantly declined germination percentage in barley

Treatment
Salinity stresse1
Outcome
germination percentagee1
Direction
declinede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Salinity stress significantly declined germination percentage, length and fresh weight of root and shoot, chlorophyll content”

✓ located verbatim in the source · abstract, characters 902–1027 · show in text ↑
t0

“four contrasting barley genotypes under salinity stress”

✓ located verbatim in the source · title, characters 68–123 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Salinity stress significantly declined root and shoot length and fresh weight of barley seedlings

Treatment
Salinity stresse1
Outcome
length and fresh weight of root and shoote1
Direction
declinede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Salinity stress significantly declined germination percentage, length and fresh weight of root and shoot, chlorophyll content”

✓ located verbatim in the source · abstract, characters 902–1027 · show in text ↑
t0

“four contrasting barley genotypes under salinity stress”

✓ located verbatim in the source · title, characters 68–123 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Salinity stress significantly declined chlorophyll content in barley seedlings

Treatment
Salinity stresse1
Outcome
chlorophyll contente1
Direction
declinede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Salinity stress significantly declined germination percentage, length and fresh weight of root and shoot, chlorophyll content”

✓ located verbatim in the source · abstract, characters 902–1027 · show in text ↑
t0

“four contrasting barley genotypes under salinity stress”

✓ located verbatim in the source · title, characters 68–123 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Salinity stress increased thiobarbituric acid reactive material, catalase activity and proline in barley roots and shoots

Treatment
Salinity stresse1
Outcome
thiobarbituric acid reactive material (TBARM), catalase activity, and proline valuese2
Direction
increasede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Salinity stress significantly declined germination percentage, length and fresh weight of root and shoot, chlorophyll content”

✓ located verbatim in the source · abstract, characters 902–1027 · show in text ↑
e2

“thiobarbituric acid reactive material (TBARM), catalase activity, and proline values were increased in root and shoot of all genotypes at seedling stage”

✓ located verbatim in the source · abstract, characters 1034–1186 · show in text ↑
t0

“four contrasting barley genotypes under salinity stress”

✓ located verbatim in the source · title, characters 68–123 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Higher values of the traits, except TBARM, were observed in salt-tolerant barley genotypes

Material
tolerant genotypese3
Outcome
these traitse3
Direction
highere3
Qualifier
except TBARMe3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e3

“the higher value of these traits except TBARM were observed in tolerant genotypes”

✓ located verbatim in the source · abstract, characters 1275–1356 · show in text ↑
t0

“four contrasting barley genotypes under salinity stress”

✓ located verbatim in the source · title, characters 68–123 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The salinity-induced changes in the traits became larger with increasing salt concentration

Treatment
increasing the salt concentratione4
Outcome
aforementioned traitse4
Direction
more changede4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e4

“The aforementioned traits were more changed with increasing the salt concentration”

✓ located verbatim in the source · abstract, characters 1188–1270 · show in text ↑
t0

“four contrasting barley genotypes under salinity stress”

✓ located verbatim in the source · title, characters 68–123 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Salt stress affected relative expression of HvNHX3, HvCAT1 and HvTIP2;3 in barley seedlings

Treatment
salt stresse5
Gene
HvNHX3, HvCAT1, HvTIP2;3e5
Outcome
expressione5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e5

“expression of HvNHX3, HvCAT1, HvTIP2;3 and Hv3ARK were affected by salt stress”

✓ located verbatim in the source · abstract, characters 1506–1584 · show in text ↑
t0

“four contrasting barley genotypes under salinity stress”

✓ located verbatim in the source · title, characters 68–123 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Salt stress affected relative expression of Hv3ARK in barley seedlings

Treatment
salt stresse5
Gene
Hv3ARKe5
Outcome
expressione5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e5

“expression of HvNHX3, HvCAT1, HvTIP2;3 and Hv3ARK were affected by salt stress”

✓ located verbatim in the source · abstract, characters 1506–1584 · show in text ↑
t0

“four contrasting barley genotypes under salinity stress”

✓ located verbatim in the source · title, characters 68–123 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

HvNHX3, HvCAT1, HvTIP2;3 and Hv3ARK play a role in barley tolerance to mild and severe salinity stress

Outcome
tolerance to mild and severe salinity stresse6
Qualifier
based on their expression patterne6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e6

“these genes play role in barley tolerance to mild and severe salinity stress based on their expression pattern”

✓ located verbatim in the source · abstract, characters 1589–1699 · show in text ↑
t0

“four contrasting barley genotypes under salinity stress”

✓ located verbatim in the source · title, characters 68–123 · show in text ↑

salinity · physiology: pigments · Hordeum vulgare (barley)Positive and negative results

18 papers: 2 positive, 16 negative or no effect

15 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. positive
  3. positive

14 papers more in this group

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salinity · growth · Hordeum vulgare (barley)Positive and negative results

103 papers: 14 positive, 89 negative or no effect

88 papers in the same direction as this paper · 14 in the opposite direction

  1. negative
  2. positive
  3. positive

99 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.