Abstract
Jasmonic acid (JA) plays an important role in regulating leaf senescence. However, the molecular mechanisms of leaf senescence in apple (Malus domestica) remain elusive. In this study, we found that MdZAT10, a C2H2-type zinc finger transcription factor (TF) in apple, markedly accelerates leaf senescence and increases the expression of senescence-related genes. To explore how MdZAT10 promotes leaf senescence, we carried out liquid chromatography/mass spectrometry screening. We found that MdABI5 physically interacts with MdZAT10. MdABI5, an important positive regulator of leaf senescence, significantly accelerated leaf senescence in apple. MdZAT10 was found to enhance the transcriptional activity of MdABI5 for MdNYC1 and MdNYE1, thus accelerating leaf senescence. In addition, we found that MdZAT10 expression was induced by methyl jasmonate (MeJA), which accelerated JA-induced leaf senescence. We also found that the JA-responsive protein MdBT2 directly interacts with MdZAT10 and reduces its protein stability through ubiquitination and degradation, thereby delaying MdZAT10-mediated leaf senescence. Taken together, our results provide new insight into the mechanisms by which MdZAT10 positively regulates JA-induced leaf senescence in apple.
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Claims 10
Claim · model-written summary
MdZAT10 accelerated apple leaf senescence.
- Taxon
- apple
Malus domesticae0 - Gene
- MdZAT10e0
- Outcome
- leaf senescencee0
- Direction
- acceleratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdZAT10, a C2H2-type zinc finger transcription factor (TF) in apple, markedly accelerates leaf senescence”
Claim · model-written summary
MdZAT10 increased expression of senescence-related genes.
- Taxon
- appleMalus domesticae0
- Gene
- MdZAT10e0
- Outcome
- expression of senescence-related genese0
- Direction
- increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdZAT10, a C2H2-type zinc finger transcription factor (TF) in apple, markedly accelerates leaf senescence and increases the expression of senescence-related genes”
Claim · model-written summary
MdABI5 physically interacted with MdZAT10.
- Taxon
- appleMalus domesticat0
- Gene
- MdABI5e0
- Outcome
- physically interacts with MdZAT10e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We found that MdABI5 physically interacts with MdZAT10”
t0“apple”
Claim · model-written summary
MdABI5 significantly accelerated apple leaf senescence.
- Taxon
- appleMalus domesticae0
- Gene
- MdABI5e0
- Outcome
- leaf senescencee0
- Direction
- acceleratede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdABI5, an important positive regulator of leaf senescence, significantly accelerated leaf senescence in apple”
Claim · model-written summary
MdZAT10 enhanced MdABI5 transcriptional activity for MdNYC1 and MdNYE1, accelerating leaf senescence.
- Taxon
- appleMalus domesticat0
- Gene
- MdZAT10e0
- Outcome
- leaf senescencee0
- Qualifier
- transcriptional activity of MdABI5 for MdNYC1 and MdNYE1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdZAT10 was found to enhance the transcriptional activity of MdABI5 for MdNYC1 and MdNYE1, thus accelerating leaf senescence”
t0“apple”
Claim · model-written summary
Methyl jasmonate induced MdZAT10 expression.
- Taxon
- appleMalus domesticat0
- Treatment
- methyl jasmonate (MeJA)e0
- Gene
- MdZAT10e0
- Outcome
- MdZAT10 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdZAT10 expression was induced by methyl jasmonate (MeJA)”
t0“apple”
Claim · model-written summary
Methyl jasmonate accelerated JA-induced leaf senescence.
- Taxon
- appleMalus domesticat0
- Treatment
- methyl jasmonate (MeJA)e0
- Outcome
- JA-induced leaf senescencee0
- Direction
- acceleratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“methyl jasmonate (MeJA), which accelerated JA-induced leaf senescence”
t0“apple”
Claim · model-written summary
MdBT2 directly interacted with MdZAT10.
- Taxon
- appleMalus domesticat0
- Gene
- MdBT2e0
- Outcome
- directly interacts with MdZAT10e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the JA-responsive protein MdBT2 directly interacts with MdZAT10”
t0“apple”
Claim · model-written summary
MdBT2 reduced MdZAT10 stability through ubiquitination and degradation.
- Taxon
- appleMalus domesticat0
- Gene
- MdBT2e0
- Outcome
- protein stabilitye0
- Direction
- reducese0
- Qualifier
- ubiquitination and degradatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdBT2 directly interacts with MdZAT10 and reduces its protein stability through ubiquitination and degradation”
t0“apple”
Claim · model-written summary
MdBT2 delayed MdZAT10-mediated leaf senescence.
- Taxon
- appleMalus domesticat0
- Gene
- MdBT2e0
- Outcome
- MdZAT10-mediated leaf senescencee0
- Direction
- delayinge0
- Qualifier
- ubiquitination and degradatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdBT2 directly interacts with MdZAT10 and reduces its protein stability through ubiquitination and degradation, thereby delaying MdZAT10-mediated leaf senescence”
t0“apple”
The same questions in other papers
(-)-methyl jasmonate · quality: postharvest · Malus domestica (apple)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
(-)-methyl jasmonate · molecular: expression · Malus domestica (apple)Results agree
6 papers in the same direction as this paper
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.