Abstract
The plant hormone ethylene is critical for ripening in climacteric fruits, including apple (Malus domestica). Jasmonate (JA) promotes ethylene biosynthesis in apple fruit, but the underlying molecular mechanism is unclear. Here, we found that JA-induced ethylene production in apple fruit is dependent on the expression of MdACS1, an ACC synthase gene involved in ethylene biosynthesis. The expression of MdMYC2, encoding a transcription factor involved in the JA signaling pathway, was enhanced by MeJA treatment in apple fruits, and MdMYC2 directly bound to the promoters of both MdACS1 and the ACC oxidase gene MdACO1 and enhanced their transcription. Furthermore, MdMYC2 bound to the promoter of MdERF3, encoding a transcription factor involved in the ethylene-signaling pathway, thereby activating MdACS1 transcription. We also found that MdMYC2 interacted with MdERF2, a suppressor of MdERF3 and MdACS1. This protein interaction prevented MdERF2 from interacting with MdERF3 and from binding to the MdACS1 promoter, leading to increased transcription of MdACS1. Collectively, these results indicate that JA promotes ethylene biosynthesis through the regulation of MdERFs and ethylene biosynthetic genes by MdMYC2.
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Claims 8
Claim · model-written summary
JA-induced ethylene production depends on MdACS1 expression.
- Taxon
- apple
Malus domesticae0 - Gene
- MdACS1e0
- Compound
- JAe0
- Outcome
- ethylene productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JA-induced ethylene production in apple fruit is dependent on the expression of MdACS1”
Claim · model-written summary
MeJA enhanced MdMYC2 expression in apple fruit.
- Taxon
- appleMalus domesticae0
- Treatment
- MeJA treatmente0
- Gene
- MdMYC2e0
- Outcome
- expression of MdMYC2e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of MdMYC2, encoding a transcription factor involved in the JA signaling pathway, was enhanced by MeJA treatment in apple fruits”
Claim · model-written summary
MdMYC2 bound the MdACS1 promoter and enhanced its transcription.
- Taxon
- appleMalus domesticat0
- Gene
- MdMYC2e0
- Outcome
- transcriptione0
- Direction
- enhancede0
- Qualifier
- promoters of both MdACS1 and the ACC oxidase gene MdACO1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdMYC2 directly bound to the promoters of both MdACS1 and the ACC oxidase gene MdACO1 and enhanced their transcription”
t0“Apple”
Claim · model-written summary
MdMYC2 bound the MdACO1 promoter and enhanced its transcription.
- Taxon
- appleMalus domesticat0
- Gene
- MdACO1e0
- Outcome
- transcriptione0
- Direction
- enhancede0
- Qualifier
- MdMYC2 directly bounde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdMYC2 directly bound to the promoters of both MdACS1 and the ACC oxidase gene MdACO1 and enhanced their transcription”
t0“Apple”
Claim · model-written summary
MdMYC2 bound the MdERF3 promoter, activating MdACS1 transcription.
- Taxon
- appleMalus domesticat0
- Gene
- MdERF3e0
- Outcome
- MdACS1 transcriptione0
- Direction
- activatinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdMYC2 bound to the promoter of MdERF3, encoding a transcription factor involved in the ethylene-signaling pathway, thereby activating MdACS1 transcription”
t0“Apple”
Claim · model-written summary
MdMYC2 interacted with MdERF2.
- Taxon
- appleMalus domesticat0
- Gene
- MdERF2e0
- Outcome
- MdMYC2 interacted with MdERF2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdMYC2 interacted with MdERF2, a suppressor of MdERF3 and MdACS1”
t0“Apple”
Claim · model-written summary
MdMYC2 interaction prevented MdERF2 from interacting with MdERF3.
- Taxon
- appleMalus domesticat0
- Gene
- MdERF2e0
- Outcome
- MdERF2 from interacting with MdERF3e0
- Qualifier
- This protein interactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This protein interaction prevented MdERF2 from interacting with MdERF3”
t0“Apple”
Claim · model-written summary
MdMYC2 interaction prevented MdERF2 binding to the MdACS1 promoter, increasing MdACS1 transcription.
- Taxon
- appleMalus domesticat0
- Gene
- MdACS1e0
- Outcome
- transcription of MdACS1e0
- Direction
- increasede0
- Qualifier
- binding to the MdACS1 promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from binding to the MdACS1 promoter, leading to increased transcription of MdACS1”
t0“Apple”
The same questions in other papers
(-)-methyl jasmonate · molecular: expression · Malus domestica (apple)Results agree
6 papers in the same direction as this paper
- positive
- positive
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3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.