Abstract
Anther culture technique is the most viable and efficient method of producing homozygous doubled haploid plants within a short period. However, the practical application of this technology in rice improvement is still limited by various factors that influence culture efficiency. The present study was conducted to determine the effects of two improved anther culture media, Ali-1 (A1) and Ali-2 (A2), a modified N6 medium, to enhance the callus formation and plant regeneration of japonica, indica, and hybrids of indica and japonica cross. The current study demonstrated that genotype and media had a significant impact (p < 0.001) on both callus induction frequency and green plantlet regeneration efficiency. The use of the A1 and A2 medium significantly enhanced callus induction frequency of japonica rice type, Nipponbare, and the hybrids of indica × japonica cross (CXY6, CXY24, and Y2) but not the indica rice type, NSIC Rc480. However, the A1 medium is found superior to the N6 medium as it significantly improved the green plantlet regeneration efficiency of CXY6, CXY24, and Y2 by almost 36%, 118%, and 277%, respectively. Furthermore, it substantially reduced the albino plantlet regeneration of the induced callus in two hybrids (CXY6 and Y2). Therefore, the improved anther culture medium A1 can produce doubled haploid rice plants for indica × japonica, which can be useful in different breeding programs that will enable the speedy development of rice varieties for resource-poor farmers.
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Claims 9
Claim · model-written summary
Genotype and medium significantly affected callus induction and green plantlet regeneration.
- Taxon
- Rice
Oryza sativat0 - Material
- genotypee0
- Outcome
- callus induction frequency and green plantlet regeneration efficiencye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genotype and media had a significant impact (p < 0.001) on both callus induction frequency and green plantlet regeneration efficiency”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
A1 significantly improved callus induction in Nipponbare.
- Taxon
- riceOryza sativae0
- Material
- Nipponbaree0
- Treatment
- A1e0
- Outcome
- callus induction frequencye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of the A1 and A2 medium significantly enhanced callus induction frequency of japonica rice type, Nipponbare, and the hybrids of indica × japonica cross (CXY6, CXY24, and Y2) but not the indica rice type, NSIC Rc480.”
Claim · model-written summary
A2 significantly improved callus induction in Nipponbare.
- Taxon
- riceOryza sativae0
- Material
- Nipponbaree0
- Treatment
- A2e0
- Outcome
- callus induction frequencye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of the A1 and A2 medium significantly enhanced callus induction frequency of japonica rice type, Nipponbare, and the hybrids of indica × japonica cross (CXY6, CXY24, and Y2) but not the indica rice type, NSIC Rc480.”
Claim · model-written summary
A1 and A2 improved callus induction in hybrid lines, but not NSIC Rc480.
- Taxon
- riceOryza sativae0
- Material
- CXY6, CXY24, and Y2e0
- Treatment
- A1 and A2 mediume0
- Outcome
- callus induction frequencye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of the A1 and A2 medium significantly enhanced callus induction frequency of japonica rice type, Nipponbare, and the hybrids of indica × japonica cross (CXY6, CXY24, and Y2) but not the indica rice type, NSIC Rc480.”
Claim · model-written summary
The media did not enhance callus induction in NSIC Rc480.
- Taxon
- riceOryza sativae0
- Material
- NSIC Rc480e0
- Treatment
- A1 and A2 mediume0
- Outcome
- callus induction frequencye0
- Direction
- but note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of the A1 and A2 medium significantly enhanced callus induction frequency of japonica rice type, Nipponbare, and the hybrids of indica × japonica cross (CXY6, CXY24, and Y2) but not the indica rice type, NSIC Rc480.”
Claim · model-written summary
A1 improved green plantlet regeneration of CXY6 by almost 36% over N6.
- Taxon
- RiceOryza sativat0
- Material
- CXY6e0
- Treatment
- A1 mediume0
- Outcome
- green plantlet regeneration efficiencye0
- Direction
- improvede0
- Value
- 36%e0
- Comparator
- N6 mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the A1 medium is found superior to the N6 medium as it significantly improved the green plantlet regeneration efficiency of CXY6, CXY24, and Y2 by almost 36%, 118%, and 277%, respectively”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
A1 improved green plantlet regeneration of CXY24 by 118% over N6.
- Taxon
- RiceOryza sativat0
- Material
- CXY24e0
- Treatment
- A1 mediume0
- Outcome
- green plantlet regeneration efficiencye0
- Direction
- improvede0
- Value
- 118%e0
- Comparator
- N6 mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the A1 medium is found superior to the N6 medium as it significantly improved the green plantlet regeneration efficiency of CXY6, CXY24, and Y2 by almost 36%, 118%, and 277%, respectively”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
A1 improved green plantlet regeneration of Y2 by 277% over N6.
- Taxon
- RiceOryza sativat0
- Material
- Y2e0
- Treatment
- A1 mediume0
- Outcome
- green plantlet regeneration efficiencye0
- Direction
- improvede0
- Value
- 277%e0
- Comparator
- N6 mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the A1 medium is found superior to the N6 medium as it significantly improved the green plantlet regeneration efficiency of CXY6, CXY24, and Y2 by almost 36%, 118%, and 277%, respectively”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
A1 reduced albino plantlet regeneration in CXY6 and Y2.
- Taxon
- RiceOryza sativat0
- Material
- CXY6 and Y2e0
- Treatment
- A1e1
- Outcome
- albino plantlet regeneratione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it substantially reduced the albino plantlet regeneration of the induced callus in two hybrids (CXY6 and Y2)”
t0“Rice (Oryza sativa L.)”
e1“the A1 medium is found superior to the N6 medium”
The same questions in other papers
in vitro · growth · Oryza sativa (rice)Positive and negative results
139 papers in the same direction as this paper · 24 in the opposite direction · 1 reporting no difference from an alternative
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