Abstract
Rice double haploid (DH) plants are produced mainly through anther culture. In order to improve the anther culture protocol, microspores of two japonica rice genotypes (NRVC980385 and H28) were subjected to three growth regulator combinations and four colchicine treatments on induction medium. In addition, a post anther culture procedure using colchicine or oryzalin was tested to induce double haploid plantlets from haploid plantlets. A cold pre-treatment of microspores for 9 days at 10 °C increased callus induction 50-fold in the NRCV980385 genotype. For both genotypes, 2 mg L−1 2,4-D and 1 mg L−1 kinetin on colchicine-free induction medium gave the best culture responses. The culturability of both genotypes changed on colchicine-supplemented induction media. A high genotype dependency was recorded for callus induction, callus regenerating green plantlets and regeneration of green double haploid plantlets. Colchicine at 300 mg L−1 for 48 h enhanced callus induction 100-fold in H28. Colchicine-supplemented media clearly improved green double haploid plantlet regeneration. We showed that the post-anther culture treatment of haploid plantlets at 500 mg L−1 of colchicine permitted fertile double haploid plantlets to be generated. Finally, an enhanced medium-throughput flow cytometry protocol for rice was tested to analyse all the plantlets from anther and post anther culture.
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Claims 7
Claim · model-written summary
Cold pre-treatment of microspores at 10 °C for 9 days increased callus induction 50-fold in genotype NRCV980385.
- Taxon
- rice
Oryza sativat0 - Material
- NRCV980385e1
- Treatment
- A cold pre-treatment of microspores for 9 days at 10 °Ce1
- Outcome
- callus inductione1
- Direction
- increasede1
- Value
- 50-folde1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mediterranean japonica rice”
e1“A cold pre-treatment of microspores for 9 days at 10 °C increased callus induction 50-fold in the NRCV980385 genotype”
Claim · model-written summary
The 2 mg L−1 2,4-D and 1 mg L−1 kinetin medium gave the best culture responses in both genotypes.
- Taxon
- riceOryza sativat0
- Treatment
- 2 mg L−1 2,4-D and 1 mg L−1 kinetine2
- Outcome
- culture responsese2
- Direction
- beste2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mediterranean japonica rice”
e2“For both genotypes, 2 mg L−1 2,4-D and 1 mg L−1 kinetin on colchicine-free induction medium gave the best culture responses”
Claim · model-written summary
The culturability of both genotypes changed on colchicine-supplemented induction media.
- Taxon
- riceOryza sativat0
- Treatment
- colchicine-supplemented induction mediae3
- Outcome
- culturabilitye3
- Direction
- changede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mediterranean japonica rice”
e3“The culturability of both genotypes changed on colchicine-supplemented induction media”
Claim · model-written summary
Colchicine at 300 mg L−1 for 48 h enhanced callus induction 100-fold in H28.
- Taxon
- riceOryza sativat0
- Material
- H28e4
- Treatment
- Colchicine at 300 mg L−1 for 48 he4
- Outcome
- callus inductione4
- Direction
- enhancede4
- Value
- 100-folde4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mediterranean japonica rice”
e4“Colchicine at 300 mg L−1 for 48 h enhanced callus induction 100-fold in H28”
Claim · model-written summary
Colchicine-supplemented media clearly improved green double haploid plantlet regeneration.
- Taxon
- riceOryza sativat0
- Treatment
- Colchicine-supplemented mediae5
- Outcome
- green double haploid plantlet regeneratione5
- Direction
- improvede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mediterranean japonica rice”
e5“Colchicine-supplemented media clearly improved green double haploid plantlet regeneration”
Claim · model-written summary
Callus induction and plantlet regeneration showed a high genotype dependency.
- Taxon
- riceOryza sativat0
- Outcome
- genotype dependencye6
- Qualifier
- callus inductione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mediterranean japonica rice”
e6“A high genotype dependency was recorded for callus induction, callus regenerating green plantlets and regeneration of green double haploid plantlets”
Claim · model-written summary
Post-anther culture treatment of haploid plantlets with 500 mg L−1 colchicine permitted fertile double haploid plantlets to be generated.
- Taxon
- riceOryza sativat0
- Treatment
- post-anther culture treatment of haploid plantlets at 500 mg L−1 of colchicinee7
- Outcome
- fertile double haploid plantletse7
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mediterranean japonica rice”
e7“We showed that the post-anther culture treatment of haploid plantlets at 500 mg L−1 of colchicine permitted fertile double haploid plantlets to be generated”
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