Abstract
This article describes the effect of Gautheret, White, Heller and Murashige & Skoog mineral salts during in-vitro clonal micropropagation of new grape varieties. The optimal mineral compositions of the culture medium that support the in-vitro regeneration of isolated grape explants were identified. The grapes that were studied were the Bart and Augustine varieties. Primary grape explants were cultivated for 30 days in a non-transplanted culture. Increased regenerative activity was observed in the Murashige & Skoog and White media. Increased haemogenesis occurred and shoots regenerated. The addition of cytokinin 6-BAP to the medium for obtaining aseptic culture led to an increase in the frequency of shoot-bud production by 5 to 6 times, depending on the type of medium. Combining 6-BAP with the auxin NAA provided an additional increase in the frequency of shoot-bud production, but to a lesser extent. Adding growth regulators to the culture medium also reduced the frequency of explant necrosis. Keywords: grapes, mineral salts, culture medium, microclonal propagation, in-vitro, cytokinins, auxins
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Claims 5
Claim · model-written summary
Regenerative activity of grape explants was increased in Murashige & Skoog and White media.
Verbatim
e0“The grapes that were studied were the Bart and Augustine varieties”
e1“Increased regenerative activity was observed in the Murashige & Skoog and White media”
Claim · model-written summary
Increased haemogenesis occurred in the grape explants and shoots regenerated.
Verbatim
e0“The grapes that were studied were the Bart and Augustine varieties”
e1“Increased haemogenesis occurred and shoots regenerated”
Claim · model-written summary
Adding the cytokinin 6-BAP to the medium for obtaining aseptic culture increased the frequency of shoot-bud production in grape by 5 to 6 times, depending on the medium type.
- Taxon
- grapese0
- Treatment
- addition of cytokinin 6-BAPe1
- Outcome
- frequency of shoot-bud productione1
- Direction
- increasee1
- Value
- 5 to 6 timese1
- Qualifier
- depending on the type of mediume1
Not reportedComparator
Verbatim
e0“The grapes that were studied were the Bart and Augustine varieties”
e1“The addition of cytokinin 6-BAP to the medium for obtaining aseptic culture led to an increase in the frequency of shoot-bud production by 5 to 6 times, depending on the type of medium”
Claim · model-written summary
Combining 6-BAP with the auxin NAA gave an additional increase in the frequency of shoot-bud production in grape, but to a lesser extent.
- Taxon
- grapese0
- Treatment
- Combining 6-BAP with the auxin NAAe1
- Outcome
- frequency of shoot-bud productione1
- Direction
- increasee1
- Qualifier
- to a lesser extente1
Not reportedComparator
Verbatim
e0“The grapes that were studied were the Bart and Augustine varieties”
e1“Combining 6-BAP with the auxin NAA provided an additional increase in the frequency of shoot-bud production, but to a lesser extent”
Claim · model-written summary
Adding growth regulators to the culture medium reduced the frequency of explant necrosis in grape.
- Taxon
- grapese0
- Treatment
- Adding growth regulators to the culture mediume1
- Outcome
- frequency of explant necrosise1
- Direction
- reducede1
Not reportedComparator
Verbatim
e0“The grapes that were studied were the Bart and Augustine varieties”
e1“Adding growth regulators to the culture medium also reduced the frequency of explant necrosis”
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