Abstract
In many cases the diagnosis of a viral disease in perennial woody plants requires indexing by grafting on indicator plants. In the case of grapevine leafroll and corky-bark diseases, indexing requires 2 to 3 years for symptom recording. Both diseases are found in all grapevine-growing countries. It would therefore be advantageous to develop a sensitive, quick, and reliable diagnostic technique. Explant shoots infected with corky-bark were micrografted onto healthy indicator rootstocks and maintained in vitro. Typical corky-bark symptoms appeared on the indicator within 8 to 12 weeks. Osmotic stress, in vitro, induced by sorbitol, enhanced leafroll symptoms. Explants expressed symptoms after 2 to 3 months of growth on these media. The advantages of these techniques are: Rapid indexing, saving of space and labor, could be performed year-round. Further experiments are underway for adaptation of the micrografting to leafroll disease and the stress method for corky-bark disease.
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Claims 4
Claim · model-written summary
Corky-bark symptoms appeared on the indicator within 8 to 12 weeks, whereas indexing by grafting requires 2 to 3 years.
- Outcome
- Typical corky-bark symptoms appeared on the indicatore1
- Value
- 8 to 12 weekse1
- Comparator
- indexing requires 2 to 3 yearse2
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Typical corky-bark symptoms appeared on the indicator within 8 to 12 weeks”
e2“In the case of grapevine leafroll and corky-bark diseases, indexing requires 2 to 3 years for symptom recording”
Claim · model-written summary
Osmotic stress induced by sorbitol in vitro enhanced grapevine leafroll symptoms.
- Treatment
- Osmotic stress, in vitro, induced by sorbitole3
- Outcome
- leafroll symptomse3
- Direction
- enhancede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“grapevine leafroll and corky-bark diseases”
e3“Osmotic stress, in vitro, induced by sorbitol, enhanced leafroll symptoms”
Claim · model-written summary
Explants expressed grapevine disease symptoms after 2 to 3 months of growth on the in vitro media.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“grapevine leafroll and corky-bark diseases”
e4“Explants expressed symptoms after 2 to 3 months of growth on these media”
Claim · model-written summary
The in vitro indexing method allows rapid indexing that saves space and labour and can be done year-round.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“grapevine leafroll and corky-bark diseases”
e2“In the case of grapevine leafroll and corky-bark diseases, indexing requires 2 to 3 years for symptom recording”
e5“Rapid indexing, saving of space and labor, could be performed year-round”
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See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.